Genomics, proteomics, and vaccines / editor, Guido Grandi.

Format:

Book

Contributor:

Grandi, Guido.

Language:

English

Subjects (All):

Genomics.

Vaccines--Biotechnology.

Vaccines.

Proteomics.

Physical Description:

1 online resource (345 p.)

Edition:

1st ed.

Place of Publication:

Chichester ; Hoboken, NJ : Wiley, c2004.

Language Note:

English

Summary:

While the sequence of the human genome sequence has hit the headlines, extensive exploitation of this for practical applications is still to come. Genomic and post-genomic technologies applied to viral and bacterial pathogens, which are almost equally important from a scientific perspective, have the potential to be translated into useful products and processes much more rapidly.Genomics, Proteomics and Vaccines introduces the history of vaccinology and discusses how vaccines are expected to evolve in the future. It describes the relevant technologies, including genome sequencing and a

Contents:

Genomics, Proteomics and Vaccines

Contents

Preface

List of contributors

PART 1: INTRODUCTION

1 Vaccination: Past, Present and Future

1.1 Introduction

1.2 Vaccination: the past

1.3 Vaccination: the present

1.4 Vaccination: the future

1.5 Conclusion: the intangible value of vaccination

2 Bioinformatics, DNA Microarrays and Proteomics in Vaccine Discovery: Competing or Complementary Technologies?

2.1 Introduction

2.2 From genome sequence to vaccine discovery

2.3 A case study: the anti-meningococcus B vaccine

2.4 Comparison of the three approaches

2.5 Conclusions: a 'nomics' approach to vaccine discovery

PART 2: TECHNOLOGIES

3 Genome Sequencing and Analysis

3.1 Introduction

3.2 Genome sequencing

3.3 Genome analysis

3.4 Conclusion

4 Understanding DNA Microarrays: Sources and Magnitudes of Variances in DNA Microarray Data Sets

4.1 Introduction

4.2 DNA array formats

4.3 Data analysis methods

4.4 Sources and magnitudes of noise in DNA microarray experiments

4.5 Conclusions

Acknowledgements

5 The Proteome, Anno Domini Two Zero Zero Three

5.1 Introduction

5.2 Some definitions

5.3 What methods exist to tackle the proteome complexity?5.4 Quantitative proteomics

5.5 Pre-fractionation in proteome analysis

5.6 Multi-dimensional chromatography

5.7 Protein chip arrays

5.8 Imaging mass spectrometry

6 Mass Spectrometry in Proteomics

6.1 Introduction

6.2 MS technology

6.3 Principle of protein identification based on MS data

6.4 Proteomics workflows

7 High Throughput Cloning, Expression and Purification Technologies

7.1 Introduction

7.2 Gene cloning

7.3 Protein expression

7.4 High-throughput protein purification

7.5 Validation of the pipeline and outlook7.6 Conclusion

PART 3: APPLICATIONS

8 Meningococcus B: from Genome to Vaccine

8.1 Meningococcus, a major cause of bacterial meningitis

8.2 Group B meningococcus as an example of reverse vaccinology

8.3 Conclusions

9 Vaccines Against Pathogenic Streptococci

9.1 Introduction

9.2 Comparative genomics of streptococci

9.3 A vaccine against group B streptococcus

9.4 A vaccine against group A streptococcus

9.5 Conclusions

10 Identification of the 'Antigenome' - a Novel Tool for Design and Development of Subunit Vaccines Against Bacterial Pathogens

10.1 Introduction

10.2 Small DNA insert libraries - a tool to cover a pathogen's 'antigenome'

10.3 Proper display platforms

10.4 Selected human sera to provide imprints of pathogen encounters

10.5 Cognate antibodies reveal the 'antigenome' of a pathogen

10.6 How to retrieve from the 'antigenome' the candidate antigens for vaccine development

10.7 Summary and discussion

11 Searching the Chlamydia Genomes for New Vaccine Candidates

11.1 Old problems and new perspectives for chlamydial vaccines.

Notes:

Description based upon print version of record.

Includes bibliographical references and index.

Description based on metadata supplied by the publisher and other sources.

Other Format:

Print version: Genomics, proteomics, and vaccines

ISBN:

9786610269488

9781280269486

1280269480

9780470093924

0470093927

9780470012536

0470012536

OCLC:

475901132