Bioluminescence : characteristics, adaptations and biotechnology / David J. Rodgerson, editor.

Format:

Book

Contributor:

Rodgerson, David J.

Series:

Biochemistry research trends series.

Biochemistry research trends

Language:

English

Subjects (All):

Bioluminescence.

Physical Description:

1 online resource (191 p.)

Edition:

1st ed.

Place of Publication:

New York : Nova Science Publishers, c2011.

Language Note:

English

Summary:

This book presents current research data in the study of the characteristics, adaptations and biotechnology of bioluminescence. Topics discussed include ATP-Driven bioluminescence; the coupling of luciferases to nanomaterials; the characteristics and adaptations of bioluminescent bacterial biosensors for acyl-HSL signal detection, quantification and tentative identification of acyl-HSLs; using bacterial biosensors to determine the toxicity of nanomaterials and other xenobiotics; and, the visual spectral mechanisms and bioluminescent optical signaling in beetles.

Contents:

Intro

CONTENTS

PREFACE

BIOLUMINESCENCE AND APPLICATIONS IN DENTISTRY

ABSTRACT

I. INTRODUCTION

A. ATP-Driven Bioluminescence and Microbiological Detection

B. Advantages and Limitations in the Use of ATP-Driven Bioluminescence Microbiological Detection

1. Simplicity

2. Accuracy

3. Efficiency

4. Specificity

C. Applications of ATP-Driven Bioluminescence in Medicine

D. Dental Caries and Use of ATP-Driven Bioluminescence in Dentistry

E. Biochemical and Microbiological Determinants of Dental Caries

II. METHODS

A. Use of ATP-Driven Bioluminescence to Assess Dental Caries Risk in Pediatric Patients

B. Clinical Observations, Plaque Index Score and Caries Activity

C. Plaque Specimens Collected from Specific Teeth

D. Microbiological Identification of Plaque and Saliva Bacteria

E. ATP-Driven Bioluminescence Determinations from Plaque and Saliva Specimens

II. RESULTS

A. Calibration of ATP Standards to Bioluminescence Measurements

B. Statistical Correlations Linking ATP-Driven Bioluminescence Values to Total Oral Bacteria, Total Oral Streptococci and Total Mutans Streptococc Numbers

III. DISCUSSION

A. Criteria Used for Development of OHSU Pediatric Dentistry Caries Risk Index

B. Quantitative Formulation of OHSU Pediatric Dentistry Caries Risk Index

C. Relationship between ATP-Driven Bioluminescence, Oral Hygiene and Caries Risk

CONCLUSION

ACKNOWLEDGMENTS

REFERENCES

NEW METHODOLOGIES BASED ON THE COUPLING OF LUCIFERASE WITH NANOMATERIALS

INTRODUCTION

Basic Concepts about Nanoscience, Nanomaterials, Nanochemistry and Nanotechnology

Why Nanomaterials-Luciferase Coupling?

Carbon-Based Nanomaterials

Carbon Nanotubes

Nanodiamonds.

Metallic Nanoparticles

Gold Nanoparticles

Quantum Dots

Nanostructured Materials

Mesoporous Silica Nanoparticles

Nanostructured Film

BIOLUMINESCENCE BIOREPORTER STRAINS FOR THE DETECTION OF QUORUM-SENSING N-ACYL-HOMOSERINE LACTONE SIGNAL MOLECULES

ABBREVIATIONS

1. INTRODUCTION

1.1. Non-specific

1.2. Semi-specific

1.3. Specific

1.4. Whole-cell Bioreporters Based on the luxCDABE Bioluminescence Output

2. CELL-TO-CELL COMMUNICATIONIN GRAM-NEGATIVE BACTERIA

2.1. Gram-negative Bacterian-acyl-homoserine Lactone(acyl-HSL)Signals

2.2. LuxR-type acyl-HSL-specific Receptor Proteins

3. BIOREPORTERSBASED ON REPORTER GENES luxCDABE FOR ACYL-HSL DETECTION

3.1. The Importance of a Control Strain

3.2. Detection of Short-chain acyl-HSLs

3.3. Detection of Long-chain Acyl-HSLs

3.4. Bioluminescent Bioreportersfor Broad Range Detection of Acyl-HSLs

3.5. Use of Acyl-HSL-specific Bioluminescent Bioreporters

3.6. Design of in vitro Cell-free Bioassay for Acyl-HSLDetection

3.7. Design of Portable Bioreporter for Acyl-HSL Detection

3.8. Potential Steps in the Detection Pathway to Increase Sensitivity and Future Directions

Diffusion of Acyl-HSLs

Use of LuxR Solos asReceptorsin acyl-HSL Biosensors

Bioengineered LuxR Receptors

Other than the luxCDABEgenes as the Bioluminescence Reporter

THE USE OF BACTERIAL BIOSENSORS TO DETERMINE THE TOXICTY OF NANOMATERIALS AND OTHER XENOBIOTICS

Bacterial Biosensors

Nanoparticles Toxicity

Phenol Compounds

2. MATERIAL AND METHODS

2.1. Chemicals

Silver Species

2.2. Media and Growth Conditions

Organism

2.3. Biosensor Assay

2.4. Calculation of IC50 (IC- Inhibitory Concentration) Values.

2.5. Data Analysis

3. RESULTS AND DISCUSSION

Silver Materials

BIOLUMINESCENCE: CHARACTERISTICS, ADAPTATIONS AND BIOTECHNOLOGY

ACKNOWLEDGMENT

BIOLUMINESCENCE RESONANT ENERGY TRANSFER AS A BASIS FOR A NOVEL HYPOXIA BIOSENSOR FOR IN VIVO FUNCTIONAL IMAGING

Imaging Techniques in Oncology

Optical Methods Overview

Bioluminescence Resonant Energy Transfer (BRET)

Optical Methods in Clinical Surgery

Hypoxia as a TumoralAggresivity Marker in Cancer

RESULTS

KINETICALLY MONITORING THE EXPRESSION OF ALPHA-TOXIN USING AN HLA PROMOTER- LUCIFERASE REPORTER FUSION IN STAPHYLOCOCCUS AUREUS

STAPHYLOCOCCAL ALPHA-TOXIN

BIOLUMINESCENCE REPORTER

KINETICALLY MONITORING THE EXPRESSION OF ALPHA-TOXIN USING A HLA PROMOTER-LUX REPORTER FUSION

BRET-BASED SCREENING ASSAYS IN SEVEN-TRANSMEMBRANE (7TM) RECEPTOR DRUG DISCOVERY

7TMRS AND ARRESTINS

BRET PRINCIPLE AND TECHNOLOGY

BRET2 SIGNAL - CLASS A VS. CLASS B 7TMRS

BRET2 SIGNAL - EFFECT OF ΒETA-ARRESTIN2 MUTANTS

BRET2 SIGNAL - EFFECT OF CARBOXYL-TAIL SWAPPING

CHARACTERISTICS AND CONTROL OF BACTERIAL BIOLUMINESCENCE

2. BIOLUMINESCENT BACTERIA

3. STABILITY OF BIOLUMINESCENCE INTENSITY

4. ANALYSIS OF THE STABILITY

5. OSCILLATION IN BACTERIAL LUMINESCENCE

6. CONTROL OF BACTERIAL LUMINESCENCE

INDEX.

Notes:

Description based upon print version of record.

Includes bibliographical references and index.

Description based on print version record.

ISBN:

1-62081-687-3

OCLC:

828869720