Caco-2 cells and their uses / Megan A. Schulz, editor.

Format:

Book

Contributor:

Schulz, Megan A.

Series:

Cell biology research progress.

Cell biology research progress

Language:

English

Subjects (All):

Epithelial cells.

Monomolecular films.

Intestinal absorption.

Physical Description:

1 online resource (219 p.)

Edition:

1st ed.

Place of Publication:

Hauppauge, N.Y. : Nova Science Publishers, Inc., c2011.

Language Note:

English

Summary:

Presents topical research in the study of CACO-2 cells and their uses. This book discusses topics that include the application of CACO-2 cell models as a supportive in vitro tool during drug discovery and formulation; co-culture systems with CACO-2 cells to evaluate the bioactivity of plant extracts; and, more.

Contents:

Intro

CACO-2 CELLS AND THEIR USES

Library of Congress Cataloging-in-Publication Data

CONTENTS

PREFACE

Chapter 1 THE INDUSTRIAL AND ACADEMIC RATIONALE OF CACO-2 CELL MODELS TODAY

ABSTRACT

ABBREVIATIONS

1. INTRODUCTION

2. CACO-2 CELL ADVANTAGES, DRAWBACKS AND TRANSPORTER-ENZYME EXPRESSION PATTERNS

2.1. Applicability of CACO-2 Models to Predict the Fraction of Drug Absorbed in Humans

2.2. CACO-2 Transporters and Enzyme Expression and the Comparison with the In Vivo Human Expression

3. CACO-2 CELLS AS AN IN VITRO PROGNOSTIC TOOL FOR ASSESSING PHARMACOKINETIC DRUG-GARLIC INTERACTIONS

4. CACO-2 CELLS FOR THE EVALUATION OF SELF-MICROEMULSIFYING DRUG DELIVERY SYSTEMS (SMEDDS)

4.1. Self-Microemulsifying Drug Delivery Systems:The Objectives and Advantages

4.2. Studies Performed to Evaluate SMEDDS Impact on Intestinal Absorption of Drugs

4.3. The Mechanism Responsible for Increased Absorption of Furosemide Incorporated in SMEDDS

CONCLUSION

REFERENCES

Chapter 2 A CRITICAL PERSPECTIVE ON CELL LINES STUDIES IN NUTRITION: THE CASE OF INTESTINAL ABSORPTION

INTRODUCTION

RESEARCH WITH CELL LINES

CELL LINES IN INTESTINAL ABSORPTION STUDIES

NON-HUMAN CELL LINES IN INTESTINAL ABSORPTION STUDIES

IN VITRO CELL CULTURES AS AN ALTERNATIVE TO ANIMAL TESTING

INTESTINAL CELL LINES AS AN IN VITRO MODEL OF CLINICAL VALUE

ACKNOWLEDGMENTS

Chapter 3 CO-CULTURE SYSTEMS WITH CACO-2 CELLS TO EVALUATE THE BIOACTIVITY OF PLANT EXTRACTS

CO-CULTURE SYSTEMS USING CACO-2 CELLS: CELL VIABILITY AND FUNCTIONALITY

WORKING WITH NATURAL EXTRACTS: CACO-2 AS A FILTER TO SIMULATE THE HUMAN INTESTINAL BARRIER

WORKING WITH NATURAL EXTRACTS: ARE THESE FILTERED EXTRACTS BIOACTIVE?

CONCLUSION.

ACKNOWLEDGMENTS

Chapter 4 THE USE OF CACO-2 CELLS IN THE STUDY OF INTESTINAL TRANSPORT

USES OF CACO-2 CELLS

Paracellular Pathway

Transcellular Pathway

Chapter 5 CACO-2 CELLS AS AN EXPERIMENTAL MODEL FOR THE STUDY OF CELL-MATRIX INTERACTIONS AND WOUND HEALING IN INTESTINAL VILLUS CELLS

CACO-2 CELLS AND CELL-MATRIX INTERACTIONS

CACO-2 CELLS AND CELL-MATRIX INTERACTIONS IN CO-CULTURE

CACO-2 CELLS IN CO-CULTURE: A WOUND HEALING MODEL

CONCLUSIONS

Chapter 6 EFFECT OF POLYUNSATURATED FATTY ACIDS AND BILE SALTS ON BUTYRATE UPTAKE BY INTESTINAL EPITHELIAL CELLS

MATERIALS AND METHODS

CACO-2 Cell Culture

IEC-6 Cell Culture

Determination of 14C-BT uptake by CACO-2 and IEC-6 Cells

Treatment of the Cells with Compounds

Determination of Cell Viability

Protein Determination

Calculation and Statistics

Materials

RESULTS

Effect of Pufas and Bile Acids on 14C-BT Apical uptake by CACO-2 Cells

Effect of Pufas and Bile Acids on 14C-BT Apical uptake by IEC-6 Cells

Chapter 7 ANALYSIS OF BOTULINUM TOXIN: INTESTINAL EPITHELIAL BARRIER INTERACTION USING EPITHELIAL MONOLAYER CULTURE SYSTEMS

1. TRANSCYTOSIS OF BONT ACROSS CULTURED INTESTINAL EPITHELIAL MONOLAYERS

2. ADHESION OF HA TO INTESTINAL EPITHELIAL CELLS

3. DISRUPTION OF THE INTERCELLULAR EPITHELIAL BARRIER BY HA

4. POSSIBLE MECHANISMS OF BONT COMPLEX TRANSPORT ACROSS THE INTESTINAL EPITHELIAL MONOLAYER

Chapter 8 CACO-2 CELLS AND GENOTOXICITY STUDIES OF FOOD CONTAMINANTS

INTRODUCTION ON IN VITRO GENOTOXICITY TESTING.

IN VITRO HUMAN INTESTINAL CELLS: PERTINENCE FOR GENOTOXICITY TESTING OF FOOD CONTAMINANTS

USE OF CACO-2 CELLS IN GENOTOXICITY TESTS

Comet Assay

Others

CYTOKINESIS-BLOCK MICRONUCLEUS ASSAY

On Undifferentiated Cells

On Differentiated Cells: Development and Application

IMPROVEMENT OF THIS CELL MODEL TO PREDICT TOXICITY AND MECHANISTIC ENDPOINTS

Co-Cultures CACO-2/TK6

Metabolism Regulation

ACKNOWLEDGMENT

Chapter 9 CACO-2 CELLS FOR STUDYING INTESTINAL ABSORPTION MECHANISMS: COMPARISON AND CORRELATIONS WITH RAT MODEL

INTRODUCTION: IN VITRO MODELS OF INTESTINAL PERMEATION

ABSORPTION IN THE GASTROINTESTINAL TRACT: ROUTES OF PERMEATION ACROSS INTESTINAL CELLS.

IN VITRO MODELS OF THE INTESTINAL BARRIER: CELL MODELS

CACO-2

Other Cell Lines

HT29 Cells

CACO-2 CELLS FOR PREDICTING ORAL FRACTION ABSORBED: COMPARISON WITH HUMAN, RAT AND PAMPA MODELS

CACO-2 CELLS FOR THE STUDY OF DRUG ABSORPTION MECHANISMS: MODELING APPROACHES FOR PARAMETER ESTIMATION

Apparent Permeability Estimation (Sink versus Non Sink Conditions)

Mathematical Modeling of CACO-2 Data for Parameter Estimation

P versus C

Amounts versus Time

CORRELATION BETWEEN CACO-2 CELLS DATA AND IN SITU RAT DATA FOR THE STUDY OF P-GP MEDIATED SECRETION

Chapter 10 NANOCARRIER ABSORPTION STUDIES WITH CACO-2 CELLS

I. METHOD

a. Absorption Study

b. Mechanistic Studies

II. RESULTS

b. Mechanistics Study

III. DISADVANTAGE/ LIMIT OF CACO-2 TO STUDY DRUG TRANSPORT / OTHER METHOD

a. Parallel Artificial Membrane Permeation Assay (PAMPA)

b. Alternative Cell Culture Models

c. In Vivo or Ex Vivo Studies

BIBLIOGRAPHY.

Chapter 11 CACO-2 CELLS AS A MODEL TO STUDY THE INTESTINAL EFFECTS OF NON-ABSORBABLE PHYTOCHEMICALS

2. PROCYANIDINS:STRUCTURE AND METABOLISM

3. PROCYANIDINS AND THE HEALTH OF THE GASTROINTESTINAL TRACT

4. INTERACTIONS OF PROCYANIDINS WITH CACO-2 CELL MEMBRANES

5. PROCYANIDINS PROTECTCACO-2 CELLSFROM OXIDATION AND PERMEABILIZATION

6. PROCYANIDINS PROTECTCACO-2 CELLS FROM INFLAMMATION

INDEX.

Notes:

Description based upon print version of record.

Includes bibliographical references and index.

Description based on print version record; title from PDF title page, viewed (07/17/2020).

ISBN:

1-62081-613-X

OCLC:

793207133