Immunodiagnostics and patient safety / Krystyna Sztefko.

Format:

Book

Author/Creator:

Sztefko, Krystyna.

Series:

Patient safety ; v. 3.

Patient safety ; v. 3

Language:

English

Subjects (All):

Immunoassay.

Patients--Safety measures.

Patients.

Physical Description:

1 online resource (176 p.)

Edition:

1st ed.

Place of Publication:

Berlin : De Gruyter, c2011.

Language Note:

English

Summary:

Today most of immunochemistry methods for the determination of proteins, peptides, drugs, and many small molecules are fully automated, with good precision, excellent sensitivity and short reaction time. However, inaccuracy due to poor standardization and the presence of interfering substances in biological samples is still a serious and life-threatening issue. Proper validation of methods and quality assurance have little effect on frequency of occurrence of false positive or false negative results, which, if unrecognized, may lead to patient's misdiagnosis, unnecessary treatment or even unnecessary surgery. Deep knowledge of basic principles of immunochemical methods (antigen-antibody reaction, standardization, matrix effect, limit of detection, cross-reactivity, etc.), sources of analyte-independent interferences (preanalitycal errors, the presence of binding proteins, the presence of autoantibodies) and analyte-dependent interferences (presence of heterophilic antibodies, high-dose effect) are very important to understand, detect, reduce and/or eliminate the interferences. This book helps to reduce false results and, at the same time, improve patient's care and patient's safety.

Contents:

Immunochemical methods : basic principle and definitions

Immunoassay standardization

Immunoassay calibration and calibration curve-fitting

Reference intervals and immunoassay

Laboratory pre-analytical and analytical phase of immunoassay

Human natural antibodies and immunoassay

Immunoassay interferences : how to recognize, eliminate, or reduce them

Immunoassay and patient safety.

Notes:

Description based upon print version of record.

Includes bibliographical references and index.

ISBN:

9786613166289

9781283166287

1283166283

9783110249484

3110249480

OCLC:

744520373