G-protein coupled receptors : essential methods / editors, David R. Poyner, Mark Wheatley.

Format:

Book

Contributor:

Poyner, David.

Wheatley, Mark.

Language:

English

Subjects (All):

Cell receptors.

Ion channels.

Physical Description:

1 online resource (312 p.)

Edition:

1st ed.

Place of Publication:

Chichester, West Sussex, UK ; Hoboken, NJ : John Wiley & Sons, 2010.

Language Note:

English

Summary:

G-Protein Coupled Receptors (GPCRs) are not only the largest protein family in the human genome but are also the single biggest target for therapeutic agents. Research into GPCRs is therefore growing at a fast pace and the range of techniques that can be applied to GPCRs is vast and continues to grow. This book provides an invaluable bench-side guide into the best and most up-to-date techniques for current and future research on GPCRs. With contributions from leading international authorities, this book equips readers with clear and detailed protocols for both well-known and up-and-coming te

Contents:

G Protein-Coupled Receptors; Contents; Preface; Contributors; 1 Measurement of Ligand-G Protein-coupled Receptor Interactions; 1.1 Introduction; 1.2 Methods and approaches; References; 2 Second Messenger Assays for G Protein-coupled Receptors: cAMP, Ca2+, Inositol Phosphates, ERK1/2; 2.1 Introduction; 2.2 Methods and approaches; 2.3 Troubleshooting; References; 3 Use of the [35S]GTPγS Binding Assay to Determine Ligand Efficacy at G Protein-coupled Receptors; 3.1 Introduction; 3.2 Methods and approaches; 3.3 Troubleshooting; Acknowledgements; References

4 Quantitative Imaging of Receptor Trafficking4.1 Introduction; 4.2 Methods and approaches; 4.3 Troubleshooting; References; 5 Production of Recombinant G Protein-coupled Receptor in Yeast for Structural and Functional Analysis; 5.1 Introduction; 5.2 Methods and approaches; 5.3 Troubleshooting; References; 6 Monitoring GPCR-Protein Complexes Using Bioluminescence Resonance Energy Transfer; 6.1 Introduction; 6.2 Methods and approaches; 6.3 Troubleshooting; References; 7 Using Intramolecular Fluorescence Resonance Energy Transfer to Study Receptor Conformation; 7.1 Introduction

7.2 Methods and approaches7.3 Troubleshooting; References; 8 A Disulfide Cross-linking Strategy Useful for Studying Ligand-induced Structural Changes in GPCRs; 8.1 Introduction; 8.2 Methods and approaches; 8.3 General considerations, caveats and troubleshooting; References; Acknowledgements; 9 Use of Fluorescence Correlation Spectroscopy to Study the Diffusion of G Protein-coupled Receptors; 9.1 Introduction; 9.2 Methods and approaches; 9.3 Troubleshooting; References; 10 Identification and Analysis of GPCR Phosphorylation; 10.1 Introduction; 10.2 Methods; Acknowledgements; References

11 Measurement and Visualization of G Protein-coupled Receptor Trafficking by Enzyme-linked Immunosorbent Assay and Immunofluorescence11.1 Introduction; 11.2 Methods and approaches; 11.3 Troubleshooting; References; 12 Substituted Cysteine Accessibility Method (SCAM); 12.1 Introduction; 12.2 Methods and approaches; 12.3 Troubleshooting; References; 13 Homology Modelling of G Protein-coupled Receptors; 13.1 Introduction; 13.2 Methods and approaches; 13.3 Troubleshooting; 13.4 Automated methods for generating models of GPCRs; References; Appendix Site-directed Mutagenesis and Chimeras

A.1 IntroductionA.2 Why mutagenesis?; A.3 Troubleshooting; A.4 Conclusion; References; Index

Notes:

Description based upon print version of record.

Includes bibliographical references and index.

ISBN:

9786612472077

9781282472075

1282472070

9780470749210

0470749210

9780470749227

0470749229

OCLC:

554903048