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Single-molecule enzymology : nanomechanical manipulation and hybrid methods / edited by Maria Spies, University of Iowa, Iowa City, IA, United States, Yann R. Chemla, University of Illinois at Urbana-Champaign, Urbana, IL, United States.
Holman Biotech Commons - Periodicals QP601 .M49 v.1 (1955)-v.148 (1987), v.150 (1987)-v.224 (1993), v.226 (1993)-v.228 (1993), v.230 (1994)-v.242 (1994), v.244 (1994)-v.253 (1995), v.257 (1996)-v.262 (1995), v.266 (1996)-v.284 (1997), v.286 (1997)-v.305 (1999), v.332 (2001)-v.333 (2001), v.341 (2001)-v.342 (2001) v.120 1986 (cum. index to v.81-94, 96-101) v.140 1988 (cum. index to v.102-119, 121-134) v.175 1990 (cum. index to v.135-139, 141-167) v.199 1993 (cum. index to v.168-174, 176-194) v.229 1995 (cum. index to v.195-198, 200-227) v.265 1996 (cum. index to v.228, 230-262) v.285 1998 (cum. index to v.263, 264, 266-289)
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Chemistry Library - Books QP601 .M49 v.1-v.193,v.195-v.207,v.209-v.220,v.222-v.228,v.230-v.259,v.261-v.263,v.265-v.285 v.286-v.365,v.367,v.369-v.654,v.656,v.658-v.720,v.722-v.724 v.168/174,176/194-v.195/198,200/227
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LIBRA QP601 .M49 v.582
Available from offsite location
LIBRA QP601 .M49 v.1-v.217, v.219-v.220, v.222-v.288, v.291, v.299, v.303 v.306 (2000)-v.321 (2000) v.323 (2000)-v.331 (2001), v.334 (2001)-v.340 (2001), v.343 (2002)-v.390 (2004) v.320 2000 (cum. index to v.290-319) v.355 2002 (cum. index to v.321-354)
Mixed Availability
- Format:
- Book
- Series:
- Methods in enzymology ; 0076-6879 v. 582.
- Methods in enzymology, 0076-6879 ; volume 582
- Language:
- English
- Subjects (All):
- Enzymology.
- Enzymes--Research--Methodology.
- Enzymes.
- Enzyme kinetics.
- Atomic force microscopy.
- Fluorescence spectroscopy.
- Fluorescence microscopy.
- Molecular biology.
- Nanostructured materials.
- Enzymes--chemical synthesis.
- Nanotechnology.
- Enzymes--Research.
- Methodology.
- Medical Subjects:
- Enzymes--chemical synthesis.
- Nanotechnology.
- Physical Description:
- xvi, 467 pages : illustrations (some color) ; 24 cm.
- Edition:
- First edition.
- Place of Publication:
- Cambridge, MA : Academic Press is an imprint of Elsevier, 2017.
- Summary:
- This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, and nanopore and tethered particle motion.
- Contents:
- 1. How to Measure Load-Dependent Kinetics of Individual Motor Molecules Without a Force-Clamp / J. Sung, K.I. Mortensen, J.A. Spudich, H. Flyvbjerg
- 2. Studying the Mechanochemistry of Processive Cytoskeletal Motors With an Optical Trap / V. Belyy, A. Yildiz
- 3. Single-Molecule Optical-Trapping Techniques to Study Molecular Mechanisms of a Replisome / B. Sun, M.D. Wang 4. Recent Advances in Biological Single-Molecule Applications of Optical Tweezers and Fluorescence Microscopy / M. Hashemi Shabestari, A.E.C. Meijering, W.H. Roos, G.J.L. Wuite, E.J.G. Peterman
- 5. Direct Visualization of Helicase Dynamics Using Fluorescence Localization and Optical Trapping / C.-T. Lin, T. Ha
- 6. High-Resolution Optical Tweezers Combined With Single-Molecule Confocal Microscopy / K.D. Whitley, M.J. Comstock, Y.R. Chemla
- 7. Integrating Optical Tweezers, DNA Tightropes, and Single-Molecule Fluorescence Imaging: Pitfalls and Traps / J. Wang, J.T. Barnett, M.R. Pollard, N.M. Kad
- 8. Single-Stranded DNA Curtains for Studying Homologous Recombination / C.J. Ma, J.B. Steinfeld, E.C. Greene
- 9. Inserting Extrahelical Structures into Long DNA Substrates for Single-Molecule Studies of DNA Mismatch Repair / M.W. Brown, A. de la Torre, I.J. Finkelstein
- 10. Single-Molecule Insight Into Target Recognition by CRISPR
- Cas Complexes / M. Rutkauskas, A. Krivoy, M.D. Szczelkun, C. Rouillon, R. Seidel
- 11. Preparation of DNA Substrates and Functionalized Glass Surfaces for Correlative Nanomanipulation and Colocalization (NanoCOSM) of Single Molecules / C. Duboc, J. Fan, E.T. Graves, T.R. Strick
- 12. Measuring Force-Induced Dissociation Kinetics of Protein Complexes Using Single-Molecule Atomic Force Microscopy / K. Manibog, C.F. Yen, S. Sivasankar
- 13. Improved Force Spectroscopy Using Focused-Ion-Beam-Modified Cantilevers / J.K. Faulk, D.T. Edwards, M.S. Bull, T.T. Perkins
- 14. Single-Molecule Characterization of DNA
- Protein Interactions Using Nanopore Biosensors / A.H. Squires, T. Gilboa, C. Torfstein, N. Varongchayakul, A. Meller
- 15. Subangstrom Measurements of Enzyme Function Using a Biological Nanopore, SPRNT / A.H. Laszlo, I.M. Derrrington, J.H. Gundlach
- 6. Multiplexed, Tethered Particle Microscopy for Studies of DNA-Enzyme Dynamics / S. Ucuncuoglu, D.A. Schneider, E.R. Weeks, D. Dunlap, L. Finzi.
- Notes:
- Includes bibliographical references and indexes.
- ISBN:
- 9780128093108
- 0128093102
- OCLC:
- 972500569
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