Immunohistochemistry and immunocytochemistry : essential methods / edited by Simon Renshaw.

Format:

Book

Contributor:

Renshaw, S. (Simon), editor.

ebrary, Inc.

George R. Fink Memorial Fund.

Language:

English

Subjects (All):

Immunohistochemistry--Technique.

Immunohistochemistry--methods.

Medical Subjects:

Immunohistochemistry--methods.

Physical Description:

1 online resource (250 pages)

Place of Publication:

Chichester, West Sussex, UK ; Hoboken, NJ, USA : John Wiley & Sons 2017.

System Details:

text file

Summary:

Immunohistochemistry and immunocytochemistry are invaluable tools for the visualization of tissue and cellular antigens in diagnostic and biological research environments. The need to obtain accurate, reliable and reproducible results is paramount. Immunohistochemistry and Immunocytochemistry: Essential Methods is the second edition of Immunohistochemisrty: Methods Express, previously published by Scion Publishing. The book is a comprehensive research guide that describes the methodologies of immunochemistry. Each chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good theoretical information and practical advice, for immediate use at the bench. Subjects covered include, antibodies and their production; selection of reporter labels; immunochemical staining methods and experimental design (both using single and multiple reporter labels); quality assurance; automated immunochemistry; confocal microscopy and electron microscopy. This book offers a wealth of knowledge to the novice immunochemists, who wishes to understand fully the theory and practice of immunochemical staining techniques and obtain reliable and reproducible data time and time again. For the experienced immunochemist, it is a comprehensive reference guide, allowing optimization of existing immunochemical staining protocols and a resource to further increase knowledge and understanding. Book jacket.

Contents:

Chapter 1 Antibodies for Immunochemistry / Mark Cooper Cooper, Mark, Sheriden Lummas Lummas, Sheriden 1

Introduction 1

Immunogens for Antibody Production 5

Antibody Production 12

Antibody Purification 16

Fragment Antibody Preparations 20

Antibody Labelling 21

Antibody Stability and Storage 23

References 24

Chapter 2 The Selection of Reporter Labels / Judith Langenick Langenick, Judith 25

Introduction 25

Enzymatic Labels 26

Fluorescence Detection 29

References 32

Chapter 3 Immunohistochemistry and Immunocytochemistry / Simon Renshaw Renshaw, Simon 35

Specimen Formats for Immunochemistry 36

Fixation 37

Processing Tissue Blocks to Paraffin Wax 46

Microtomy 47

Tissue Microarrays 47

Specimen Storage 48

Decalcification 49

Antigen Retrieval 50

Controls 56

Immunochemical Staining Techniques (Optimizing a New Antibody) 57

Countersigns 71

Mounting 74

Troubleshooting 76

Examples of Immunostaining Photomicrographs 76

Acknowledgements 101

References 101

Chapter 4 Multiple Immunochemical Staining Techniques / Sofia Koch Koch, Sofia 103

Introduction 103

Methods and Approaches 115

References 122

Chapter 5 Quality Assurance in Immunochemistry / Peter Jackson Jackson, Peter, Michael Gandy Gandy, Michael 123

Introduction 123

Methods and Approaches 125

Automated Immunochemical Staining 147

Troubleshooting 149

References 154

Chapter 6 Automated Immunochemistry / Emanuel Schenck Schenck, Emanuel, Simon Renshaw Renshaw, Simon 157

Introduction 157

Methods and Approaches 160

Other Forms of Automation 164

References 168

Chapter 7 Confocal Microscopy / Ann Wheeler Wheeler, Ann 169

Introduction 169

When Should Confocal be Used? 173

Applications: For Example Co-ocalization, Quantification, 3D Visualization and Kinetics 173

How To Set Up a Confocal Experiment? 174

References 193

Further Readings 198

Chapter 8 Ultrastructural Immunochemistry / Jeremy Skepper Skepper, Jeremy, Janet Powell Powell, Janet 199

Introduction 199

Methods and Approaches 207

References 222.

Notes:

Electronic reproduction. Palo Alto, Calif. Available via World Wide Web.

Description based on online resource; title from digital title page (viewed on March 15, 2017).

Local Notes:

Acquired for the Penn Libraries with assistance from the George R. Fink Memorial Fund.

ISBN:

1118717759

9781118717752

1118717767

9781118717769

1118717740

9781118717745

Publisher Number:

99971133227

Access Restriction:

Restricted for use by site license.