Intermediate filament associated proteins / edited by Katherine L. Wilson, Arnoud Sonnenberg.

Format:

Book

Contributor:

Wilson, Katherine L., editor.

Sonnenberg, Arnoud, editor.

Series:

Methods in enzymology ; v. 569.

Methods in enzymology, 0076-6879 ; volume 569

Language:

English

Subjects (All):

Proteins.

Intermediate filament proteins.

Physical Description:

xx, 576 pages, 30 unnumbered pages of plates : illustrations (some color) ; 24 cm.

Edition:

First edition.

Place of Publication:

Amsterdam : Elsevier Academic Press, 2016.

Contents:

BioID identification of lamin-associated proteins

Proximity-dependent biotin identification (BioID) in Dictyostelium Amoebae

Purification and structural analysis of LEM-domain proteins

Purification and structural analysis of SUN and KASH domain proteins - Purification and lamins and soluble fragments of NETs

Simple separation of functionally distinct populations of lamin-binding proteins

Development of a novel green fluroescent protein-based binding assay to study the association of plakins with intermediate filament proteins

Functional analysis of keratin-associated proteins in intestinal epithelia: heat-shock protein chaperoning and kinase rescue

Purification of protein chaperones and their functional assays with intermediate filaments

Purification and structural analysis of plectin and BPAG1e

Purification and structural analysis of desmoplakin

Degradation of the intermediate filament family by gigaxonin

Functional and genetic analysis of plectin in skin and muscle

Functional and genetic analysis of epiplakin in epithelial cells

In vitro model of the epidermis: connecting protein function to 3D structure

Functional analysis of periplakin and envoplakin, cytoskeletal linkers, and cornified envelope precursor proteins

Microtubule-actin cross-linking factor 1: domains, interaction partners, and tissue-specific functions

Functional and genetic analysis of neuronal isoforms of BPAG1

Functional and genetic analysis of spectraplakins in Drosophila

Functional and genetic analysis of VAB-10 spectraplakin in Caenorhabditis elegans

Tagged chromosomal insertion site system: a method to study lamina-associated chromatin

Lamin-binding proteins in caenorhabditis elegans

Pathologically relevant prelamin A interactions with transcription factors

MCLIP detection of novel protein-protein interactions at the nuclear envelope

Preparation of the human cytomegalovirus nuclear egress complex and associated proteins.

Notes:

Includes bibliographical references and indexes.

ISBN:

0128034696

9780128034699

OCLC:

921864830