Intermediate filament proteins / edited by M. Bishr Omary, Ronald K.H. Liem.

Format:

Book

Contributor:

Omary, M. Bishr, editor.

Liem, Ronald K. H., editor.

Series:

Methods in enzymology ; v. 568.

Methods in enzymology, 0076-6879 ; volume five hundred and sixty eight

Language:

English

Subjects (All):

Intermediate filament proteins.

Physical Description:

xxv, 791 pages, 38 unnumbered pages of plates : illustrations (some color) ; 24 cm.

Edition:

First edition.

Place of Publication:

Amsterdam : Elsevier/Academic Press, 2016.

Contents:

How to study intermediate filaments in atomic detail

Mechanical properties of intermediate filament proteins

Multidimensional monitoring of keratin intermediate filaments in cultured cells and tissues

Phospho-specific anitbody probes of intermediate filament proteins

Assays for posttranslational modifications of intermediate filament proteins

Immunofluorescence and immunohistochemical detection of keratins - High-throughput screening for drugs that moderate intermediate filament proteins

The use of Withaferin A to study intermediate filaments

Assays to study consequences of cytoplasmic intermediate filament mutations: the case of epidermal keratins

Using data mining and computational approaches to study intermediate filament structure and function

Isolation and analysis of keratins and keratin-associated proteins from hair and wool

Skin keratins

Simple epithelial keratins

Methods for determining the cellular functions of Vimentin intermediate filaments

Strategies to study desmin in cardiac muscle and culture systems

Genetic manipulation of neurofilament protein phosphorylation

a-internexin and peripherin: expression, assembly, functions, and roles in disease

Studying nestin and its interrelationship with Cdk5

Synemin: molecular features and the use of proximity ligation assay to study its interactions

Targeting mitogen-activated protein kinase signaling in mouse models of cardiomyopathy caused by Lamin A/C gene mutations

In vivo, ex vivo, and in vitro approaches to study intermediate filaments in the eye lens

Compartment-specific phosphorylation of squid neurofilaments

Using xenopus embryos to study transcriptional and posttranscriptional gene regulatory mechanisms of intermediate filaments

Intermediate filaments in Caenorhabditis elegans

Mechanical probing of the intermediate filament-rich Caenorhabditis elegans intestine

Using Drosophila for studies of intermediate filaments.

Notes:

Includes bibliographical references and indexes.

ISBN:

012803470X

9780128034705

OCLC:

921864831