Isotope labeling of biomolecules : applications / edited by Zvi Kelman.

Format:

Book

Contributor:

Kelman, Zvi, editor.

Series:

Methods in enzymology ; v. 566.

Methods in enzymology, 0076-6879 ; volume five hundred and sixty six

Language:

English

Subjects (All):

Radiolabeling.

Biomolecules.

Physical Description:

xvi, 465 pages, 30 unnumbered pages of plates : illustrations (some color) ; 24 cm.

Edition:

First edition.

Place of Publication:

Amsterdam : Elsevier/Academic Press, 2016.

Contents:

Application of natural isotopic abundance 1H-13C-and 1H-15N-correlated two-dimensional NMR for evaluation of the structure of protein therapeutics

A semiautomated assignment protocol for methyl group side chains in large proteins

19F-site-specific-labeled nucleotides for nucleic acid structural analysis by NMR

Applying thymine isostere 2,4-difluoro-5-methylbenzene as a NMR assignment tool and probe of homopyrimidine/homopurine tract structural dynamics

Biomolecular deuteration for neutron structural biology and dynamics

Deuterium labeling together with contrast variation small-angle neutron scattering suggests how skip captures and releases unfolded outer membrane proteins

Deuteration in biological neutron reflectometry

Deuterium labeling strategies for creating contrast in structure-function studies of model bacterial outer membranes using neutron reflectometry

Essential strategies for revealing nanoscale protein dynamics by neutron spin echo spectroscopy

Method for the determination of 15N incorporation percentage in labeled peptides and proteins

QconCAT: internal standard for protein quantification

Production purification, and characterization of 15N-labeled DNA repair proteins as internal standards for mass spectrometric measurements

Hydrogen exchange mass spectrometry

Mapping protein-ligand interactions with proteolytic fragmentation, hydrogen/deuterium exchange-mass spectrometry

Isotope labelgin of biomolecules: structural analysis of viruses by HDX-MS.

Notes:

Includes bibliographical references and indexes.

ISBN:

0128030496

9780128030493

OCLC:

921864810