Isotope labeling of biomolecules : labeling methods / edited by Zvi Kelman.

Format:

Book

Contributor:

Kelman, Zvi.

Series:

Methods in enzymology ; v. 565.

Methods in enzymology, 0076-6879 ; v. 565

Language:

English

Subjects (All):

Biomolecules--Analysis.

Biomolecules.

Stable isotope tracers.

Physical Description:

xx, 614 pages, 13 pages of plates : illustrations (some color) ; 24 cm.

Place of Publication:

Amsterdam, Netherlands : Elsevier/Academic Press, 2015.

Contents:

Robust high-yield methodologies for ²H and ²H/15N/13C labeling of proteins for structural investigations using neutron scattering and NMR

Protein labeling in Escherichia coli with ²H, 13C, and 15N

Escherichia coli auxotroph host strains for amino acid-selective isotope labeling of recombinant proteins

19F-modified proteins and 19F-containing ligands as tools in solution NMR studies of protein interactions

Biopolymer deuteration for neutron scattering and other isotope-sensitive techniques

Production of bacterial cellulose with controlled deuterium-hydrogen substitution for neutron scattering studies

Isotope labeling of proteins in Halobacterium salinarum

Amino acid selective unlabeling in protein NMR spectroscopy

Isotope labeling of eukaryotic membrane proteins in yeast for solid-state NMR

Development of approaches for deuterium incorporation in plants

Isotope labeling of proteins in insect cells

Effective isotope labeling of proteins in a mammalian expression system

Escherichia coli cell-free synthesis and isotope labeling of mammalian proteins

Rapid biosynthesis of stable isotope-labeled peptides from a reconstituted in vitro translation system for targeted proteomics 347

Labeling of membrane proteins by cell-free expression

Selective amino acid segmental labeling of multi-domain proteins

Labeling monosaccharides with stable isotopes

Stable isotope-labeled RNA phosphoramidites to facilitate dynamics by NMR

In vivo, large-scale preparation of uniformly 15N- and site-specifically 13C-labeled homogeneous, recombinant RNA for NMR studies

Cut and paste RNA for nuclear magnetic resonance, paramagnetic resonance enhancement, and electron paramagnetic resonance structural studies.

Notes:

Includes bibliographical references and indexes.

ISBN:

0128030488

9780128030486

OCLC:

913923613

Publisher Number:

9780128030486