Plant Microtechniques and Protocols / edited by Edward Chee Tak Yeung, Claudio Stasolla, Michael John Sumner, Bing Quan Huang.

Format:

Book

Contributor:

Yeung, Edward Chee Tak, editor.

Stasolla, Claudio, editor.

Sumner, Michael John, editor.

Huang, Bing Quan, editor.

SpringerLink (Online service)

Language:

English

Subjects (All):

Life sciences.

Plant anatomy.

Plants--Development.

Plants.

Plant breeding.

Physical Description:

1 online resource

Edition:

First edition 2015.

Contained In:

Springer eBooks

Place of Publication:

Cham : Springer International Publishing : Imprint: Springer, 2015.

System Details:

Mode of Access: World Wide Web.

text file PDF

Summary:

This work gathers common plant microtechniques and updates their procedures using a simple and fully understandable approach. The text serves as a handy resource for scientists familiar with the protocols and as a guide for the novice, especially students just beginning to learn about various structural methods for the first time. This book analyzes a range of topics in order to generate cross-talks among scientists in different research disciplines. The first section of this volume covers the more commonly used embedding methods, with emphasis on the preparative methods for light and electron microscopy. A number of cell biology related protocols are compiled in the second section to showcase the usefulness of various techniques based on different processing and staining methods. The third section highlights some common and recent procedures in wood preparation. The last section includes botanical methods related to archaeological uses of plant materials. A special chapter on field and herbarium procedures is also included to serve as a guide to students interested in plant collection and taxonomic studies.

Contents:

A Guide to the Study of Plant Structure with Emphasis on Living Specimens

Chemical and Physical Fixation of Cells and Tissues: an Overview

Paraffin and Polyester Waxes

The Glycol Methacrylate Embedding Resins - Technovit 7100 and 8100

Epoxy Resins for Light and Transmission Electron Microscopy

LR White Acrylic Resin

High Pressure Freezing and Freeze Substitution of In vivo and In vitro Cultured Plant Samples

Three-Dimensional Imaging for Electron Microscopy of Plastic-embedded Plant Specimens

Fluorescent Staining of Living Plant Cells

Improved Methods for Clearing and Staining of Plant Samples

Whole Mount Immunofluorescence Staining of Plant Cells and Tissues

Protoplast Isolation and Staining

Guiding Principles for Live Cell Imaging of Plants using Confocal Microscopy.-Immunogold Labeling for Electron Microscopy: Strategy and Problem Solving

Abscisic Acid Immunostaining

Plant Chromosome Preparations and Staining for Light Microscopic Studies

Chromosome techniques and FISH

Detection of S-phase of Cell Division Cycle in Plant Cells and Tissues by using 5-ethynyl-2'-deoxyuridine (EdU)

Staining Methods for Programmed Cell Death

Laser Microdissection of Plant Tissues

RNA In situ Hybridization

Microscopic Examination of Wood: Sample Preparation and Techniques for Light Microscopy

Collecting and Processing Wood Microcores for Monitoring Xylogenesis

Three-dimensional Imaging of Cambium and Secondary Xylem Cells by Confocal Laser Scanning Microscopy

Collection of Plant Remains from Archaeological Contexts

Archaeological Wood Preparation

Archaeopalynological Preparation Techniques

Phytoliths: Preparation and Archaeological Extraction

Starch Granules: Preparation and Archaeological Extraction

Plant Collection, Identification, and Herbarium Procedures

Index.

Other Format:

Printed edition:

ISBN:

9783319199443

Access Restriction:

Restricted for use by site license.