Structures of large RNA molecules and their complexes / edited by Sarah A. Woodson, Frédéric H.T. Allain.

Format:

Book

Contributor:

Woodson, Sarah A., editor.

Allain, Frédéric H. T., editor.

Series:

Methods in enzymology ; v. 558.

Methods in enzymology, 0076-6879 ; volume 558

Language:

English

Subjects (All):

RNA.

Physical Description:

xxi, 631 pages, 20 unnumbered pages of plates : illustrations ; 24 cm.

Edition:

First edition.

Place of Publication:

Amsterdam : Academic Press/Elsevier, 2015.

Summary:

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in RNA folding and dynamics, RNA-protein interactions and large RNPs.

Contents:

Native purification and analysis of long RNAs

Characterizing RNA excited states using NMR relaxation dispersion

Quantifying nucleic acid ensembles with x-ray scattering interferometry

2-aminopurine fluorescence as a probe of local RNA structure and dynamics and global folding

Mod-seq: a high-throughput method for probing RNA secondary structure

Reproducible analysis of sequencing-based RNA structure probing data with user-friendly tools

Computational methods for RNA structure validation and improvement

Structures of large RNAs and RNA-protein complexes: toward structure determination of riboswitches

One, two, three, four! How multiple RRMs read the genome sequence

Combining NMR and EPR to determine structures of large RNAs and protein-RNA complexes in solution

Structural analysis of protein-RNA complexes in solution using NMR paramagnetic relaxation enhancements

Resolving individual components in protein-RNA complexes using small-angle x-ray scattering experiments

Small-angle neutron scattering for structural biology of protein-RNA complexes

Studying RNA-protein interactions of Pre-mRNA complexes by mass spectrometry

RNA Bind-n-Seq: measuring the binding affinity landscape of RNA-binding proteins

Using molecular simulation to model high-resolution Cryo-EM reconstructions

In vitro reconstitution and crystallization of Cas9 endonuclease bound to a guide RNA and a DNA target

Single-molecule pull-down FRET to dissect the mechanisms of biomolecular machines

Single-molecule imaging of RNA splicing in live cells.

Notes:

Includes bibliographical references and indexes.

ISBN:

0128019344

9780128019344

OCLC:

903596287