Plant transcription factors : evolutionary, structural and functional aspects / edited by Daniel H. Gonzalez.

Format:

Book

Contributor:

González, Daniel H., editor.

ScienceDirect (Online service).

Rudolph G. Schmieder Fund.

Language:

English

Subjects (All):

Plant genetic regulation.

Plant cell differentiation.

Genetic transcription.

Physical Description:

1 online resource : illustrations (some color)

polychrome

Place of Publication:

London Wall, UK ; San Diego, CA, USA : Academic Press, an imprint of Elsevier, [2016]

System Details:

text file

Contents:

Cover; Title page; Copyright Page; Contents; List of Contributors; Preface; Section A

General Aspects of Plant Transcription Factors; Chapter 1

Introduction to Transcription Factor Structure and Function; 1.1

Introduction: Transcription in eukaryotes; 1.2

Structure of transcription factors; 1.3

DNA recognition by transcription factors; 1.4

DNA-binding domains; 1.5

Protein-protein interactions; 1.6

Regulation of transcription factor action; 1.7

Plant transcription factors; Acknowledgments; References; Chapter 2

Methods to Study Transcription Factor Structure and Function

2.1

Introduction2.2

In vivo functional studies; 2.2.1

Overexpression or Ectopic Expression of Transcription Factors; 2.2.2

Transactivation Systems for Expression of Transcription Factors; 2.2.3

Expression of Dominant Negative Forms of Transcription Factors; 2.2.4

Expression of Dysregulated Forms of Transcription Factors; 2.2.5

Gain-of-Function Mutants; 2.2.6

Expression of Fusions to Activating or Repressor Domains; 2.2.7

Inducible Systems; 2.3

Methods for the analysis of in vitro protein-DNA interactions; 2.3.1

The EMSA Assay; 2.3.2

Selex

2.3.3

Analysis of Transcription Factor-DNA Complexes by Footprinting Assays2.3.4

Microarray-Based Identification of Transcription Factor Target Genes; 2.4

Methods to study protein-DNA interactions in vivo; 2.4.1

Chromatin Immunoprecipitation (ChIP) Assays; 2.4.2

ChIP-chip and ChIP-Seq; 2.4.3

DNA Adenine Methyltransferase Identification (DamID); 2.4.4

Identification of Transcription Factors Using the Yeast One-Hybrid Assay; 2.4.5

Transient Assays to Analyze Protein-DNA Interactions In Vivo; 2.5

Analysis of protein-protein interactions

2.5.1

Methods for Protein-Protein Complex Identification2.5.1.1

Yeast Two-Hybrid Assay (Y2H); 2.5.1.2

Tandem and One-step Tag-Based Affinity Purification; 2.5.2

Methods for Verification of Protein-Protein Interactions; 2.5.2.1

Coimmunoprecipitation; 2.5.2.2

In Vivo Split Methods; 2.5.2.3

Resonance Energy Transfer Methods; Acknowledgments; References; Chapter 3

General Aspects of Plant Transcription Factor Families; 3.1

Introduction; 3.2

Overview of the transcription cycle in eukaryotes; 3.2.1

Core Promoter Elements and General Transcription Factors.

3.2.2

Role of Nucleosome-Modifying Enzymes and Chromatin Remodelers3.2.3

Productive Transcription Initiation and Elongation; 3.2.4

Transcription, Termination, and Reinitiation; 3.3

Components involved in the formation of the RNAPII preinitiation complex in plants; 3.3.1

GTFs and RNAPII; 3.3.2

Mediator; 3.3.3

Corepressors of the Plant Gro/Tup1 Family; 3.4

Plant transcription factor families; 3.5

Major TF families that are conserved across eukaryotes; 3.5.1

bZIP Transcription Factor Family; 3.5.2

bHLH TF Family; 3.5.3

MYB TF Family; 3.5.4

HSF Family

3.6

Plant-specific TF families.

Notes:

Includes bibliographical references and index.

Electronic reproduction. Amsterdam Available via World Wide Web.

Description based on online resource; title from digital title page (viewed on August 14, 2015).

Local Notes:

Acquired for the Penn Libraries with assistance from the Rudolph G. Schmieder Fund.

Other Format:

Print version: Gonzalez, Daniel H Plant Transcription Factors : Evolutionary, Structural and Functional Aspects

ISBN:

9780128011270

0128011270

Publisher Number:

99963918586

Access Restriction:

Restricted for use by site license.