Energetics of biological macromolecules / edited by Michael L. Johnson, Gary K. Ackers.

Format:

Book

Contributor:

Johnson, Michael L., 1947-

Ackers, Gary K.

Series:

Methods in enzymology ; v. 259, etc.

Methods in enzymology, 0076-6879 ; v. 259, etc

Language:

English

Subjects (All):

Macromolecules.

Bioenergetics.

Thermodynamics.

Energy Metabolism.

Macromolecular Substances.

Allosteric Regulation.

Molecular Conformation.

Medical Subjects:

Energy Metabolism.

Macromolecular Substances.

Allosteric Regulation.

Molecular Conformation.

Thermodynamics.

Genre:

Electronic books.

Physical Description:

1 online resource (volumes) : illustrations, portrait.

Place of Publication:

San Diego : Academic Press, ©1995-

System Details:

Mode of access: World Wide Web.

text file

Summary:

The critically acclaimed laboratory standard for forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerlyawaited, frequently consulted, and praised by researchers and reviewers alike. More than 250 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. Key Features * Thermodynamics as a tool for understanding molecular logic * Thermal denaturation methods in the study of protein folding * Predicting thermodynamic properties of RNA * Sedimentation equilibrium as a thermodynamic tool * Molecular volume * Thermodynamic parameters from hydrogen exchange measurements.

Contents:

1. Deciphering Rules of Helix Stability in Peptides / Carol A. Rohl, Robert L. Baldwin 1

2. Modified Amino Acids as Probes of Helix Stability / Neville R. Kallenbach, Erik J. Spek 26

3. Measuring Hydration Changes of Proteins in Solution: Applications of Osmotic Stress and Structure-Based Calculations / Vince J. LiCata, Norma M. Allewell 42

4. Protein Folding in Membranes: Determining Energetics of Peptide-Bilayer Interactions / Stephen H. White, William C. Wimley, Alexey S. Ladokhin, Kalina Hristova 62

5. Tight Ligand Binding Affinities Determined from Thermodynamic Linkage to Temperature by Titration Calorimetry / Michael L. Doyle, Preston Hensley 88

6. Structure-Based Prediction of Binding Affinities and Molecular Design of Peptide Ligands / Irene Luque, Ernesto Freire 100

7. Molecular Crowding: Analysis of Effects of High Concentrations of Inert Cosolutes on Biochemical Equilibria and Rates in Terms of Volume Exclusion / Allen P. Minton 127

8. Application of Automated Methods for Determination of Protein Conformational Stability / Jeffery M. Schwehm, Wesley E. Stites 150

9. Calculations of Proton-Binding Thermodynamics in Proteins / Paul Beroza, David A. Case 170

10. Analysis of Spectra from Multiwavelength Oxygen-Binding Studies of Mixed Metal Hybrid Hemoglobins / Alexandra L. Klinger, Gary K. Ackers 190

11. Study of the Bohr Effect in Hemoglobin Intermediates / Louise Benazzi, Rosaria Russo, Marilena Ripamonti, Michele Perrella 208

12. Application of Planck-Benzinger Relationships to Biology / Paul W. Chun 227

13. Kinetic Analysis of Macromolecular Interactions Using Surface Plasmon Resonance Biosensors / Thomas A. Morton, David G. Myszka 268

14. Prediction of Binding Energetics from Structure Using Empirical Parameterization / Brian M. Baker, Kenneth P. Murphy 294

15. Photoacoustic Calorimetry of Proteins / Mark A. McLean, Carmelo Di Primo, Eric Deprez, Gaston Hui Bon Hoa, Stephen G. Sligar 316

16. Isoergonic Cooperativity: A Novel Form of Allostery / Harvey F. Fisher, Jon Tally 331

17. Theoretical Aspects of Isothermal Titration Calorimetry / Lawrence Indyk, Harvey F. Fisher 350

18. Use of Poisson-Boltzmann Equation to Analyze Ion Binding to DNA / Victor A. Bloomfield, Ioulia Rouzina 364

19. Following the Folding of RNA with Time-Resolved Synchrotron X-Ray Footprinting / Bianca Sclavi, Sarah Woodson, Michael Sullivan, Mark Chance, Michael Brenowitz 379

20. Analysis of Interactions between CytR and CRP at CytR-Regulated Promoters / Donald F. Senear, Laura T. Perini, Stacey A. Gavigan 403

21. Energetic Methods to Study Bifunctional Biotin Operon Repressor / Dorothy Beckett 424

22. Thermal Melting Properties of C-Terminal Domain Mutants of Bacteriophage [lambda] cI Repressor / Eddine K. Merabet, David S. Burz, Gary K. Ackers 450

23. Investigation of Phase Transitions in Bilayer Membranes / Jeffrey T. Mason 468

24. Membrane-Confined Analytical Electrophoresis / Thomas M. Laue, Harvey K. Shepard, Theresa M. Ridgeway, Thomas P. Moody, Timothy J. Wilson 494.

Notes:

Includes bibliographical references and indexes.

Description based on print version record.

Other Format:

Print version: Energetics of biological macromolecules.

OCLC:

805925072

Access Restriction:

Restricted for use by site license.