Vitamins and coenzymes. Part L / edited by Donald B. McCormick, John W. Suttie, Conrad Wagner.

Format:

Book

Contributor:

McCormick, Donald B. (Donald Bruce)

Suttie, J. W. (John Weston), 1934-

Wagner, Conrad.

Series:

Methods in enzymology ; v. 282.

Methods in enzymology ; v. 282

Language:

English

Subjects (All):

Enzymology--Methods.

Enzymology.

Vitamins.

Coenzymes.

Medical Subjects:

Vitamins.

Coenzymes.

Genre:

Electronic books.

Methods (Music)

Physical Description:

1 online resource (xxxi, 505 pages) : illustrations.

Place of Publication:

San Diego : Academic Press, 1997.

System Details:

Mode of access: World Wide Web.

text file

Summary:

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 270 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. Key Features * This volume and its companion Volumes 279, 280, and 281 provide * A collation of the most recent and useful methods for the identification, preparation, and quantification of vitamins and coenzymes * Details on physical, chemical, and biological properties of vitamins and coenzymes * Chemical and biological syntheses of vitamins, coenzymes, and their analogs * Aspects of transport and metabolism of vitamins and coenzymes.

Contents:

Generation and characterization of cellular retinoic acid-binding proteins from Escherichia coli expression systems

Generation and characterizing retinoid receptors from Escherichia coli and insect cell expression systems

Expression and characterization of retinoid receptors in yeast

Use of in situ hybridization techniques to study embryonic expression of retinoid receptors and binding proteins

Use of quantitative polymerase chain reaction to study retinoid receptor expression

Use of quantitative polymerase chain reaction to study cellular retinoic acid-binding protein-II mRNA expression

Use of transgenic mice to study activation of retinoic acid-responsive promoters

Use of transgenic mice to eliminate retinoic acid receptor function

Use of reporter cells to study endogenous retinoid sources

Preparation of radiolabeled 9-cis- and all-trans-retinoids

Identification and quantification of retinoic acid and other metabolites from [beta]-Carotene excentric cleavage

Assessing metabolism of [beta]-Carotene.

Atmospheric pressure chemical ionization and electron capture negative chemical ionization mass spectrometry in studying [beta]-Carotene conversion to retinol in humans

Synthesis of Vitamin D₃ and 1[alpha],25-Dihydroxy Vitamin D₃

Assay of 1,25-dihydroxyvitamin D₃ from serum samples

Quantitation of 25-Hydroxyvitamin D and 1,25-Dihydroxyvitamin D

Assay of vitamin D derivatives and purification of vitamin D hydroxylases

Assay of 25-Hydroxyvitamin D 1[alpha]-Hydroxlase and 24-Hydroxylase

Molecular cloning of vitamin D₃ hydroxylases

Role of 1[alpha],25-dihydroxyvitamin D₃ in osteoclast differentiation and function

Assay of direct effect of 1,25-Dihydroxyvitamin D₃ on calcium ion

Vitamin E status and immune function

Inhibition of platelet adhesion as functional test for vitamin E status

Inhibition of plasma cholesterol ester hydroperoxide

[alpha]-Tocopherol-binding proteins

[alpha]-Carboxyethyl 6-Hydrozychroman.

Purification of vitamin K-dependent carboxylase from cultured cells

Purification of native bovine carboxylase

Purification of [gamma]-Glutamyl carboxylase

Assay of vitamin K-dependent carboxylase activity

Expression of human anticoagulation protein C and [gamma]-Carboxyglutamic acid mutants

Determination of site-specific [gamma]-carboxyglutamin acid formation

Purification of warfarin-sensitive vitamin K epoxide reductase

Determination of vitamin K compounds in plasma or serum

Assay of phylloquinone in plasma

Assay of menaquinones in plasma

Assay of phylloquinone and menaquinones in human liver

Determination of phylloquinone in foods

Assay of menaquinones in bacterial cultures, stool samples, and intestinal contents.

Notes:

Includes bibliographical references and indexes.

ISBN:

0121821838

9780121821838

OCLC:

438187974

Access Restriction:

Restricted for use by site license.