Green fluorescent protein / edited by P. Michael Conn.

Format:

Book

Contributor:

Conn, P. Michael.

Series:

Methods in enzymology ; 0076-6879 v. 302.

Methods in enzymology, 0076-6879 ; v. 302

Language:

English

Subjects (All):

Green fluorescent protein.

Luminescent Proteins.

Medical Subjects:

Luminescent Proteins.

Genre:

Electronic books.

Physical Description:

1 online resource (xxxii, 490 pages) : illustrations.

Place of Publication:

San Diego, Calif. ; London : Academic, [1999]

System Details:

Mode of access: World Wide Web.

text file

Summary:

One of the most important recent discoveries in science is the green fluorescent protein, isolated from a bioluminescent jellyfish. The gene that codes for this protein is active in any type of cell, from microbes to humans, producing a "day-glo" dye visible as a bright green light in the microscope. The power of the gene is that by using recombinant DNA technology. GFP can be spliced to any protein, essentially painting that protein green. When such a spliced protein is inside a cell. Further, time-lapse microscopy can be used to examine how that protein and the cellular components that it is part of move within the cell during the cell's life cycle. This Methods in Enzymology volume deals with the utility of green fluorescent protein (GFP). The OVID database (including MEDLINE, Current Contents, and other sources) lists nine references to GFP for the ten-year period 1985-1994. In contrast, in less than four years thereafter, over 500 references are listed, a testament to the rapid growth of interest in this probe. This volume documents many diverse uses for this interesting molecule in disciplines that broadly span biology. The methods presented include shortcuts and conveniences not included in previously published sources. The techniques are described in a context that allows comparisons to other related methodologies-such comparisons are valuable to readers who must adapt existing procedures to new systems. Also, so far as possible, methodologies have been presented in a manner that stresses their general applicability and potential limitations. The volume provides a substantial and current overview of the extant methodology in the field and a view of its rapid development. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences. Key Features * Monitoring of Physiological Processes * Localization of Molecules * Special Uses * Mutants * Variants of GFP.

Contents:

Monitoring of physiological processes

Localization of molecules

Special uses

Mutants and variants of green fluorescent protein.

Notes:

Includes index.

Description based on print version record.

Includes bibliographical references and indexes.

Other Format:

Print version: Green fluorescent protein.

ISBN:

0121822036

9780121822033

OCLC:

326630833

Access Restriction:

Restricted for use by site license.