Riboswitches as targets and tools / edited by Donald H. Burke-Aguero.

Format:

Book

Contributor:

Burke, Donald H.

Series:

Methods in enzymology ; v. 550.

Methods in enzymology, 0076-6879 ; volume 550

Language:

English

Subjects (All):

RNA.

RNA-protein interactions.

Messenger RNA.

Ligands (Biochemistry).

Riboswitches.

Genetic regulation.

Molecular genetics--Methodology.

Molecular genetics.

Molecular genetics--Technique.

Physical Description:

xix, 423 pages, 24 unnumbered pages : illustrations ; 24 cm.

Edition:

First edition.

Place of Publication:

Amsterdam : Academic Press/Elsevier, 2015.

Contents:

Design of transcription regulating riboswitches

Ligand-dependent exponential amplification of self-replicating RNA enzymes

Design of modular "plug-and-play" expression platforms derived from natural riboswitches for engineering novel genetically encodable RNA regulatory devices

Integrating and amplifying signal from riboswitch biosensors

Simple identification of two causes of noise in an aptazyme system by monitoring cell-free transcription

Engineering of ribosomal shunt-modulating eukaryotic ON riboswitches by using a cell-free translation system

Live-cell imaging of mammalian RNAs with spinach2

In vitro analysis of riboswitch-spinach aptamer fusions as metabolite-sensing fluorescent biosensors

Using spinach aptamer to correlate mRNA and protein levels in Eschrichia coli

Monitoring mRNA and protein levels in bulk and in model vesicle-based artificial cells

Design, synthesis and application of spinach molecular beacons triggered by strand displacement

Using riboswitches to regulate gene expression and define gene function in mycobacteria

Controlling expression of genes in the unicelleular alga Chlamydomonas reinhardtii with a vitamin-repressible riboswitch

Conditional control of gene expression by synthetic riboswitches in Streptomyces coelicolor

Engineering of ribozyme-based aminoglycoside switches of gene expression by In Vivo genetic selection in Saccharomyces cerevisiae

Kinetic folding design of aptazyme-regulated expression devices as riboswitches for metabolic engineering

Riboselector: riboswitch-based synthetic selection device to expedite evolution of metabolite-producing microorganisms

Fluorescence assays for monitoring RNA-ligand interactions and riboswitch-targeted drug discovery screening

Monitoring ribosomal frameshifting as a platform to screen anti-riboswitch drug candidates.

Notes:

Includes bibliographical references and indexes.

ISBN:

9780128011232

0128011238

OCLC:

892454752