Laboratory methods in enzymology : protein. Part C / edited by Jon Lorsch.

Format:

Book

Contributor:

Lorsch, Jon, editor.

Series:

Methods in enzymology ; v. 541.

Methods in enzymology, 0076-6879 ; volume 541

Language:

English

Subjects (All):

Proteins--Analysis.

Proteins.

Proteins--Purification.

Physical Description:

xvii, 272 pages : illustrations ; 24 cm.

Edition:

First edition.

Place of Publication:

Amsterdam ; Boston : Elsevier/Academic Press, 2014.

Contents:

Section 1: Protein protocols/protein precipiation: TCA precipitation

Section II: Protein protocols/protein pull-down methods: Coimmunoprecipitation of proteins from yeast

Coupling antibody to cyanogen bromide-activated sepharose

Analysis of protein-protein interactions by coimmunoprecipitation

Section III: Protein protocols/protein purification: Use and application of hydrophobic interaction chromatography for protein purification

Hydroxyapatite chromatography: purification strategies for recombinant proteins

Salting out of proteins using ammonium sulfate precipitation

Using ion exchange chromatography to purify a recombinantly expressed protein

Gel filtration chromatography (size exclusion chromatography) of proteins

Section IV: Protein protocols/purification of membrane proteins: Expression and purification of membrane proteins

Explantory chapter: choosing the right detergent

Section V: Protein protocols/SDS PAGE: One-dimensional SDS-polyacrylamide gel electrophoresis (1D SDS-PAGE)

Coomassie blue staining

Silver staining of SDS-polyacrylamide gel

Section VI: Protein protocols/Standard in vitro assays for protein-nucleic acid interactions: Standard in vitro assays for protein-nucleic acid interactions-gel shift assays for RNA and DNA binding

Protein filter binding

Section VII: Protein protocolstroubleshooting protein expression: Explanatory chapter: troubleshooting recombinant protein expression: general

Explanatory chapter: troubleshooting protein expression: what to do when the protein is not soluble

Section VIII: Protein protocols/Western blotting: Western blotting using chemiluminescent substrates.

Notes:

Includes bibliographical references and indexes.

ISBN:

9780124201194

0124201199

OCLC:

868642459