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Manipulating the mouse embryo : a laboratory manual / Richard Behringer, University of Texas, M.D. Anderson Cancer Center, Marina Gertsenstein, Toronto Centre for Phenogenomics, Transgenic Core, Kristina Vintersten Nagy, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Andras Nagy, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto.
Holman Biotech Commons QL737.R6 M2468 2014
Available
Veterinary: du Pont Library (New Bolton) QL737.R6 M2468 2014
Available
- Format:
- Book
- Author/Creator:
- Behringer, Richard, author.
- Gertsenstein, Marina, author.
- Nagy, Kristina Vintersten, author.
- Nagy, Andras, 1951- author.
- Language:
- English
- Subjects (All):
- Mice--Embryos--Laboratory manuals.
- Mice.
- Mice--Genetic engineering--Laboratory manuals.
- Transgenic mice--Laboratory manuals.
- Transgenic mice.
- Mice as laboratory animals.
- Mice--Genetic engineering.
- Mice--Embryos.
- Genre:
- Laboratory manuals.
- Handbooks and manuals.
- Physical Description:
- xxii, 814 pages : illustrations (some color) ; 28 cm
- Edition:
- Fourth edition.
- Place of Publication:
- Cold Spring Harbor, New York : Cold Spring Harbor Laboratory Press, [2014]
- Contents:
- Genetics and embryology of the mouse: past, present, and future
- Summary of mouse development
- A mouse colony for the production of transgenic and chimeric animals
- Recovery and in vitro culture of preimplantation embryos
- Isolation, culture, and manipulation of postimplantation embryos
- Surgical procedures
- Production of transgenic mice by pronuclear microinjection
- Embryo-derived stem cell lines
- Germline-competent stem cells derived from adult mice
- Vector designs for pluripotent stem cell-based transgenesis and genome alterations
- Introduction of foreign DNA into embryonic stem cells
- Production of chimeras
- Genotyping
- Parthenogenesis, pronuclear transfer, and mouse cloning
- Assisted reproduction: ovary transplantation, in vitro fertilization, artificial insemination, and intracytoplasmic sperm injection
- Cryopreservation, rederivation, and transport of mouse strains
- Techniques for visualizing gene products, cells, tissues, and organ systems
- Setting up a micromanipulation lab.
- Notes:
- Includes bibliographical references and index.
- ISBN:
- 9781936113002
- 1936113007
- 9781936113019
- 1936113015
- OCLC:
- 852763546
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