Cilia. Part A / edited by Wallace F. Marshall.

Format:

Book

Contributor:

Marshall, Wallace F., 1968- editor.

Akhmanova, Anna, 1967- contributor.

Alper, Joshua, contributor.

Behal, Robert H., contributor.

Brust-Mascher, Ingrid, contributor.

Bui, Khanh Huu, contributor.

Cantele, Francesca, contributor.

Chen, Christopher Y., contributor.

Cole, Douglas G., contributor.

Conti, Marco, Prof., contributor.

DiPetrillo, Christen G., contributor.

Dishinger, John F., contributor.

Dogic, Zvonimir, contributor.

Eddy, E. Mitchell (Edward Mitchell), contributor.

Follit, John A., contributor.

Geimer, Stefan, contributor.

Geyer, Veikko, contributor.

Grigoriev, Ilya, contributor.

Guptā, Añjali, contributor.

Hogan, Marie C., contributor.

Howard, Jonathon, 1957- contributor.

Huang, Kaiyao, contributor.

Ishikawa, Takashi, contributor.

Kee, H. Lynn, contributor.

King, Stephen M., contributor.

Lanzavecchia, Salvatore, contributor.

Larsen, Jesper K., contributor.

Lechtreck, Karl F., contributor.

Liu, Yi, contributor.

Long, Huan, contributor.

Lupetti, Pietro, contributor.

Mukundan, Vikram, contributor.

Ou, Guangshou, contributor.

Paccagnini, Eugenio, contributor.

Pazour, Gregory J. (Gregory Joseph), contributor.

Pedersen, Lotte B., contributor.

Pigino, Gaia, contributor.

Qin, Hongmin, contributor.

Richey, Elizabeth, contributor.

Rogowski, Michaela, contributor.

Sanchez, Timothy, contributor.

Scholey, Jonathan M., contributor.

Scholz, Dirk, contributor.

Shingyoji, Chikako, contributor.

Sivadas, Priyanka, contributor.

Smith, Elizabeth F., contributor.

Vannuccini, Elisa, contributor.

Verhey, Kristen J., contributor.

Ward, Christopher J., contributor.

Xie, Fang, contributor.

Yang, Pinfen, contributor.

Zhu, Xiaoyan, contributor.

Series:

Methods in enzymology ; v. 524.

Methods in enzymology, 0076-6879 ; v. 524

Language:

English

Subjects (All):

Cilia and ciliary motion.

Flagella (Microbiology).

Cells--Motility.

Cells.

Physical Description:

liv, 397 pages, 16 unnumbered pages of plates : illustrations (some color) ; 24 cm.

Place of Publication:

Amsterdam ; Boston : Elsevier/Academic Press, 2013.

Summary:

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cilia and includes chapters on such topics as electron microscopy of IFT in cilia and flagella, radial spoke isolation and assays, and biomechanical measurements of kinocilium. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers ciliaContains chapters on such topics as electron microscopy of IFT in cilia and flagella, radial spoke isolation.

Contents:

1. Isolation of intraflagellar transport particle proteins from Chlamydomonas reinhardtii / Elizabeth Richey and Hongmin Qin

2. Molecular tools for studying the radial spoke / Xiaoyan Zhu, Yi Liu, Priyanka Sivadas, Anjali Gupta, and Pinfen Yang

3. Methods for analysis of calcium/calmodulin signaling in cilia and flagella / Christen G. DiPetrillo and Elizabeth F. Smith

4. Analysis of flagellar protein ubiquitination / Huan Long and Kaiyao Huang

5. Analysis of ciliary import / John F. Dishinger, H. Lynn Kee, and Kristen J. Verhey

6. Analysis of signaling pathways controlling flagellar movements in mammalian spermatozoa / Fang Xie, Edward M. Eddy, and Marco Conti

7. Analysis of microtubule plus-end-tracking proteins in cilia / Jesper Larsen, Ilya Grigoriev, Anna Akhmanova, and Lotte B. Pedersen

8. Biochemical and physiological analysis of axonemal dyneins / Stephen M. King

9. Measuring the regulation of dynein activity during flagellar motility / Chikako Shingyoji

10. Analysis of interactions between intraflagellar transport proteins / Robert H. Behal and Douglas G. Cole

11. Analysis of ciliary membrane protein dynamics using SNAP technology / John A. Follit and Gregory J. Pazour

12. Engineering oscillating microtubule bundles / Timothy Sanchez and Zvonimir Dogic

13. Purification of exosome-like vesicles from urine / Christopher Y. Chen, Marie C. Hogan, and Christopher J. Ward

14. Electron microscopy of flagella, primary cilia, and intraflagellar transport in flat-embedded cells / Michaela Rogowski, Dirk Scholz, and Stefan Geimer

15. In vivo imaging of IFT in Chlamydomonas flagella / Karl F. Lechtreck

16. Measuring rates of intraflagellar transport along Caenorhabditis elegans sensory cilia using fluorescence microscopy / Ingrid Brust-Mascher, Guangshuo Ou, and Jonathan M. Scholey

17. 3D structural analysis of flagella/cilia by cryo-electron tomography / Khanh Huy Bui and Takashi Ishikawa

18. Electron tomography of IFT particles / Gaia Pigino, Francesca Cantele, Elisa Vannuccini, Salvatore Lanzavecchia, Eugenio Paccagnini, and Pietro Lupetti

19. Reconstitution of flagellar sliding / Joshua Alper, Veikko Geyer, Vikram Mukundan, and Jonathon Howard.

Notes:

Includes bibliographical references and indexes.

ISBN:

9780123984869

0123984866

9780123979452

0123979455

OCLC:

841671905