Ion exchange chromatography of proteins / Shuichi Yamamoto, Kazuhiro Nakanishi, Ryuichi Matsuno.

Format:

Book

Author/Creator:

Yamamoto, Shūichi, 1953-

Contributor:

Nakanishi, Kazuhiro, 1945-

Matsuno, Ryūichi, 1939-2022.

Series:

Chromatographic science ; v. 43.

Chromatographic science ; v. 43

Language:

English

Subjects (All):

Chromatography, Ion Exchange--methods.

Proteins--analysis.

Proteins--Analysis.

Proteins.

Ion exchange chromatography.

Medical Subjects:

Chromatography, Ion Exchange--methods.

Proteins--analysis.

Physical Description:

vii, 401 pages : illustrations ; 24 cm.

Place of Publication:

New York : M. Dekker, [1988]

Summary:

Showing chemists how to predict ion-exchange chromatography (IEC) separation behavior and how to determine appropriate operating conditions, this reference illustrates procedures, apparatus, and types of ion exchangers, emphasizing the design and application of large-scale IEC. Complete with more than 100 useful tables and diagrams, Ion-Exchange Chromatography of Proteins explains the effects of each variable on separation behavior ... compares models and equations describing separation behavior of proteins in IEC with experimental results ... introduces design calculation procedures for scale up, adapting IEC for individual requirements ... discusses parameters affecting separation behavior, including adsorption equilibria, stationary phase diffusion, and axial dispersion ... provides examples of the most up-to-date applications, such as high- or medium-performance IEC and large-scale operations ... and reviews both theoretical and experimental literature. Ion-Exchange Chromatography of Proteins serves as an important reference for analytical, agricultural, food, and pharmaceutical chemists, chromatographers, food scientists and technologists, biochemists, and biotechnologists.

Contents:

1.2 Basic Concepts of Column Chromatography 8

1.3 Brief History of Ion-Exchange Chromatography 15

1.4 Comparison of Ion-Exchange Chromatography with Other Chromatographic Techniques 20

1.5 Characteristics of Ion-Exchange Chromatography of Proteins 27

1.6 Various Elution Procedures of Ion-Exchange Chromatography of Proteins 30

2 Theoretical Aspects 35

2.2 Theory of Ion-Exchange Chromatography of Proteins 78

3 Ion-Exchange Equilibria 107

3.1 Theoretical Treatment 108

3.2 Experimental Methods 121

3.3 Experimental Data 125

4 Diffusion in the Ion Exchanger 145

4.1 Theoretical Treatment 145

4.2 Experimental Methods 151

4.3 Experimental Data 156

5 Axial Dispersion in the Ion-Exchange Column 161

6 Experimental Methods and Apparatus 171

6.2 Pretreatment 179

6.3 Preparation of Ion-Exchange Gel Columns 180

6.4 Method for Sample Introduction 183

6.5 Method for Elution 186

6.6 Regeneration of the Column 187

6.7 Concentration of Fractions 187

7 Ion Exchangers 193

8 Factors Affecting Separation Behavior 209

8.1 Elution Behavior in Linear Gradient Elution 210

8.2 Prediction of Peak Position and Peak Width in Linear Gradient Elution by Simplified Methods 226

8.3 Resolution in Linear Gradient Elution 238

8.4 Stepwise Elution 265

8.5 Other Special Separation Methods and Apparatus 277

9 Large-Scale Operation 305

9.2 Applications 317

9.3 Problems Encountered in Actual Operation 326

10 Design Calculation Procedure 329

10.1 Linear Gradient Elution 330

10.2 Stepwise Elution 341

A Derivation of the Value of K[subscript S] 349

B Derivation of Equation (2.59) 351

C Analytical Solutions for a Change in Ionic Strength in Stepwise Elution and Linear Gradient Elution 352

D Numerical Calculation Procedure 354

E A Solution for the Peak Width in Linear Gradient Elution Under the Quasi-Steady State 355

F Values of the Molecular Diffusion Coefficient and its Estimation Method 359.

Notes:

Includes index.

Bibliography: pages 377-393.

ISBN:

0824779037

OCLC:

17478400