Solutions manual for Introduction to genetic analysis, tenth edition / David Scott ... [and others].

Format:

Book

Contributor:

Scott, David.

Class of 1924 Book Fund.

Language:

English

Subjects (All):

Introduction to genetic analysis.

Genetics--Problems, exercises, etc.

Genetics.

Genre:

Problems and exercises.

Physical Description:

475 pages : illustrations ; 28 cm

Other Title:

Introduction to genetic analysis, tenth edition

Place of Publication:

New York : W H. Freeman & Co., [2012]

Contents:

Machine generated contents note: ch. 1 The Genetics Revolution in the Life Sciences

1.1.The Nature of Biological Information

The molecular structure of DNA

DNA is organized into genes and chromosomes

1.2.How Information Becomes Biological Form

Transcription

Translation

How does life replicate itself?

Change at the DNA level

1.3.Genetics and Evolution

Natural selection

Constructing evolutionary lineages

1.4.Genetics Has Provided a Powerful New Approach to Biological Research

Forward genetics

Reverse genetics

Manipulating DNA

Detecting specific sequences of DNA, RNA, and protein

1.5.Model Organisms Have Been Crucial in the Genetics Revolution

1.6.Genetics Changes Society

1.7.Genetics and the Future

pt. I Transmission Genetics

ch. 2 Single-Gene Inheritance

2.1.Single-Gene Inheritance Patterns

Mendel's pioneering experiments

Mendel's law of equal segregation

2.2.The Chromosomal Basis of Single-Gene Inheritance Patterns

Single-gene inheritance in diploids

Single-gene inheritance in haploids

2.3.The Molecular Basis of Mendelian Inheritance Patterns

Structural differences between alleles at the molecular level

Molecular aspects of gene transmission

Alleles at the molecular level

2.4.Some Genes Discovered by Observing Segregation Ratios

A gene active in the development of flower color

A gene for wing development

A gene for hyphal branching

Predicting progeny proportions or parental genotypes by applying the principles of single-gene inheritance

2.5.Sex-Linked Single-Gene Inheritance Patterns

Sex chromosomes

Sex-linked patterns of inheritance

X-linked inheritance

2.6.Human Pedigree Analysis

Autosomal recessive disorders

Autosomal dominant disorders

Autosomal polymorphisms

X-linked recessive disorders

X-linked dominant disorders

Y-linked inheritance

Calculating risks in pedigree analysis

ch. 3 Independent Assortment of Genes

3.1.Mendel's Law of Independent Assortment

3.2.Working with Independent Assortment

Predicting progeny ratios

Using the chi-square test on monohybrid and dihybrid ratios

Synthesizing pure lines

Hybrid vigor

3.3.The Chromosomal Basis of Independent Assortment

Independent assortment in diploid organisms

Independent assortment in haploid organisms

Independent assortment of combinations of autosomal and X-linked genes

Recombination

3.4.Polygenic Inheritance

3.5.Organelle Genes: Inheritance Independent of the Nucleus

Patterns of inheritance in organelles

Cytoplasmic segregation

Cytoplasmic mutations in humans

MtDNA in evolutionary studies

ch. 4 Mapping Eukaryote Chromosomes by Recombination

4.1.Diagnostics of Linkage

Using recombinant frequency to estimate linkage

How crossovers produce recombinants for linked genes

Linkage symbolism and terminology

Evidence that crossing over is a breakage-and-rejoining process

Evidence that crossing over takes place at the four-chromatid stage

Multiple crossovers can include more than two chromatids

4.2.Mapping by Recombinant Frequency

Map units

Three-point testcross

Deducing gene order by inspection

Interference

Using ratios as diagnostics

4.3.Mapping with Molecular Markers

Single nucleotide polymorphisms

Simple sequence length polymorphisms

Detecting simple sequence length polymorphism

Recombination analysis using molecular markers

4.4.Centromere Mapping with Linear Tetrads

4.5.Using the Chi-Square Test for Testing Linkage Analysis

4.6.Accounting for Unseen Multiple Crossovers

A mapping function

The Perkins formula

4.7.Using Recombination-Based Maps in Conjunction with Physical Maps

4.8.The Molecular Mechanism of Crossing Over

ch. 5 The Genetics of Bacteria and Their Viruses

5.1.Working with Microorganisms

5.2.Bacterial Conjugation

Discovery of conjugation

Discovery of the fertility factor (F)

Hfr strains

Mapping of bacterial chromosomes

F plasmids that carry genomic fragments

R plasmids

5.3.Bacterial Transformation

The nature of transformation

Chromosome mapping using transformation

5.4.Bacteriophage Genetics

Infection of bacteria by phages

Mapping phage chromosomes by using phage crosses

5.5.Transduction

Discovery of transduction

Generalized transduction

Specialized transduction

Mechanism of specialized transduction

5.6.Physical Maps and Linkage Maps Compared

ch. 6 Gene Interaction

6.1.Interactions Between the Alleles of a Single Gene: Variations on Dominance

Complete dominance and recessiveness

Incomplete dominance

Codominance

Recessive lethal alleles

6.2.Interaction of Genes in Pathways

Biosynthetic pathways in Neurospora

Gene interaction in other types of pathways

6.3.Inferring Gene Interactions

Sorting mutants using the complementation test

Analyzing double mutants of random mutations

6.4.Penetrance and Expressivity

pt. II From DNA to Phenotype

ch. 7 DNA: Structure and Replication

7.1.DNA: The Genetic Material

Discovery of transformation

Hershey

Chase experiment

7.2.The DNA Structure

DNA structure before Watson and Crick

The double helix

7.3.Semiconservative Replication

Meselson-Stahl experiment

The replication fork

DNA polymerases

7.4.Overview of DNA Replication

7.5.The Replisome: A Remarkable Replication Machine

Unwinding the double helix

Assembling the replisome: replication initiation

7.6.Replication in Eukaryotic Organisms

The eukaryotic replisome

Eukaryotic origins of replication

DNA replication and the yeast cell cycle

Replication origins in higher eukaryotes

7.7.Telomeres and Telomerase: Replication Termination

ch. 8 RNA: Transcription and Processing

8.1.RNA

Early experiments suggest an RNA intermediate

Properties of RNA

Classes of RNA

8.2.Transcription

Overview: DNA as transcription template

Stages of transcription

8.3.Transcription in Eukaryotes

Transcription initiation in eukaryotes

Elongation, termination, and pre-mRNA processing in eukaryotes

8.4.Intron Removal and Exon Splicing

Small nuclear RNAs (snRNAs): The mechanism of exon splicing

Self-splicing introns and the RNA world

8.5.Small Functional RNAs that Regulate and Protect the Eukaryotic Genome

miRNAs are important regulators of gene expression

siRNAs ensure genome stability

Similar mechanisms generate siRNA and miRNA

ch. 9 Proteins and Their Synthesis

9.1.Protein Structure

9.2.The Genetic Code

Overlapping versus nonoverlapping codes

Number of letters in the codon

Use of suppressors to demonstrate a triplet code

Degeneracy of the genetic code

Cracking the code

Stop codons

9.3.tRNA: The Adapter

Codon translation by tRNA

Degeneracy revisited

9.4.Ribosomes

Ribosome features

Translation initiation, elongation, and termination

Nonsense suppressor mutations

9.5.The Proteome

Alternative splicing generates protein isoforms

Posttranslational events

ch. 10 Gene Isolation and Manipulation

10.1.Overview: Isolating and Amplifying Specific DNA Fragments

10.2.Generating Recombinant DNA Molecules

Genomic DNA can be cut up before cloning

The polymerase chain reaction amplifies selected regions of DNA in vitro

DNA copies of mRNA can be synthesized

Attaching donor and vector DNA

Amplification of donor DNA inside a bacterial cell

Making genomic and cDNA libraries

10.3.Finding a Specific Clone of Interest

Finding specific clones by using probes

Finding specific clones by functional complementation

Southern- and Northern-blot analysis of DNA

10.4.Determining the Base Sequence of a DNA Segment

10.5.Aligning Genetic and Physical Maps to Isolate Specific Genes

Using positional cloning to identify a human-disease gene

Using fine-mapping to identify genes

10.6.Genetic Engineering

Genetic engineering in Saccharomyces cerevisiae

Genetic engineering in plants

Genetic engineering in animals

ch.

11 Regulation of Gene Expression in Bacteria and Their Viruses

11.1.Gene Regulation

The basics of prokaryotic transcriptional regulation: genetic switches

A first look at the lac regulatory circuit

11.2.Discovery of the lac System: Negative Control

Genes controlled together

Genetic evidence for the operator and repressor

Genetic evidence for allostery

Genetic analysis of the lac promoter

Molecular characterization of the Lac repressor and the lac operator

Polar mutations

11.3.Catabolite Repression of the lac Operon: Positive Control

The basics of lac catabolite repression: choosing the best sugar to metabolize

The structure of DNA target sites

A summary of the lac operon

11.4.Dual Positive and Negative Control: The Arabinose Operon

11.5.Metabolic Pathways and Additional Levels of Regulation: Attenuation

11.6.Bacteriophage Life Cycles: More Regulators, Complex Operons

Molecular anatomy of the genetic switch

Sequence-specific binding of regulatory proteins to DNA

11.7.Alternative Sigma Factors Regulate Large Sets of Genes

ch. 12 Regulation of Gene Expression in Eukaryotes

12.1.Transcriptional Regulation in Eukaryotes: An Overview

12.2.Lessons from Yeast: The GAL System

Gal4 regulates multiple genes through upstream activation sequences

The Gal4 protein has separable DNA-binding and activation domains

Gal4 activity is physiologically regulated

Gal4 functions in most eukaryotes

Activators recruit the transcriptional machinery

The control of yeast mating type: combinatorial interactions

12.3.Dynamic Chromatin

Chromatin-remodeling proteins and gene activation

Histones and chromatin remodeling

The inheritance of histone modifications and chromatin structure

Local Notes:

Acquired for the Penn Libraries with assistance from the Class of 1924 Book Fund.

ISBN:

9781429232555

1429232552

OCLC:

748266924