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Culture of animal cells : a manual of basic technique and specialized applications / R. Ian Freshney.

Veterinary: Atwood Library (Campus) QH585.2 .F74 2010
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Format:
Book
Author/Creator:
Freshney, R. Ian.
Language:
English
Subjects (All):
Tissue culture--Laboratory manuals.
Tissue culture.
Cell culture--Laboratory manuals.
Cell culture.
Cells, Cultured.
Culture Techniques--methods.
Medical Subjects:
Cells, Cultured.
Culture Techniques--methods.
Local Subjects:
Tissue culture--Laboratory manuals.
Cell culture--Laboratory manuals.
Genre:
Laboratory manuals.
Physical Description:
xxxi, 732 pages, 28 unnumbered pages of plates : illustrations (some color) ; 29 cm
Edition:
Sixth edition.
Place of Publication:
Hoboken, N.J. : Wiley-Blackwell, [2010]
Contents:
Introduction
Biology of Cultured Cells
Laboratory Design, Layout, and Equipment
Equipment and Materials
Aseptic Technique
Safety, Bioethics, and Validation
Culture Vessels and Substrates
Defined Media and Supplements
Serum-Free Media
Preparation and Sterilization
Primary Culture
Subculture and Cell Lines
Cloning and Selection
Cell Separation
Characterization
Differentiation
Transformation and Immortalization
Contamination
Cryopreservation
Quantitation
Cytotoxicity
Specialized Cells
Stem Cells, Germ Cells, and Amniocytes
Culture of Tumor Cells
Three-Dimensional Culture
Scale-up and Automation
Specialized Techniques
Training Programs
Problem Solving
In Conclusion
Notes:
Includes bibliographical references and index.
ISBN:
9780470528129
0470528125
OCLC:
553361168

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