Protein purification : principles, high resolution methods, and applications / edited by Jan-Christer Janson.

Format:

Book

Contributor:

Janson, Jan-Christer.

Series:

Methods of biochemical analysis ; v. 54.

Methods of biochemical analysis

Language:

English

Subjects (All):

Proteins--Purification.

Proteins.

Chromatographic analysis.

Electrophoresis.

Physical Description:

xiv, 517 pages : illustrations (some color) ; 29 cm.

Edition:

Third edition.

Place of Publication:

Hoboken, N.J. : John Wiley & Sons, [2011]

Contents:

Introduction to protein purification / B. Ersson, L. Rydén and J.-C. Janson

Introduction to chromatography / J.-C. Janson and J.Å. Jönsson

Gel filtration: Size exclusion chromatography / L. Hagel

Ion exchange chromatography / E. Karlsson and I. Hirsh

High-resolution reversed-phase chromatography of proteins / S.W. Pettersson

Hydrophobic interaction chromatography / K.-O. Eriksson and M. Belew

Immobilized metal ion affinity chromatography / L. Kågedal

Covalent chromatography / F. Batista-Viera, L. Rydén and J. Carlsson

Affinity chromatography / F. Batista-Viera, J.-C. Janson and J. Carlsson

Affinity ligands from chemical combinatorial libraries / E. Carredano and H. Baumann

Affinity ligands from biological combinatorial libraries / P.-Å. Nygren

Membrane separations / J.K. Walter ... [et al.]

Refolding of inclusion body proteins from E. coli / Z. Su, D. Lu and Z. Liu

Purification of PEGylated proteins / C.J. Fee and J.M. Van Alstine

Electrophoresis in gels / R. Westermeier

Conventional isoelectric focusing in gel slabs and capillaries and immobilized pH gradients / P.G. Righetti, E. Fasoli and S.C. Righetti

Two-dimensional electrophoresis in proteomics / R. Westermeier and A. Görg

Protein elution and blotting techniques / R. Westermeier

Capillary electrophoretic separations / W. Thormann

High throughput screening techniques in protein purification / K.M. Lacki and E. Brekkan.

Notes:

Includes bibliographical references and index.

ISBN:

9780471746614

0471746614

OCLC:

653842887