Characterization of B cell development and activation in the absence of Akt or presenilin.

Format:

Book

Thesis/Dissertation

Author/Creator:

Calamito, Marco.

Contributor:

Allman, David, advisor.

University of Pennsylvania.

Language:

English

Subjects (All):

Immunology.

0982.

Penn dissertations--Immunology.

Immunology--Penn dissertations.

Local Subjects:

Penn dissertations--Immunology.

Immunology--Penn dissertations.

0982.

Physical Description:

132 pages

Contained In:

Dissertation Abstracts International 71-12B.

System Details:

Mode of access: World Wide Web.

text file

Summary:

The biochemical pathways critical to B cell development remain poorly defined. Here I characterize a critical role for two separate families of proteins, Akt and Presenilin in the development and activation of B cells. The absence of Akt1 and Akt2 leads to a block in marginal zone (MZ) and B1B cell development, as well as decreased cellularity of splenic follicular B cells. In addition, I find the combined loss of Akt1 and Akt2 causes altered B cell receptor repertoire and poor competitive ability when matched against wild-type B cells. Similar to deficiencies in the Akt pathway the combined loss of Presenilin1 and Presenilin2 results in defective MZ, B1B cell development, and altered BCR repertoire selection. Furthermore, I find that these defects are independent of the Notch pathway and that Presenilins are required for optimal responses to cross-linking of the BCR. Collectively, these findings identify and phenotypically characterize two novel pathways important to B cell development and function.

Notes:

Thesis (Ph.D. in Immunology) -- University of Pennsylvania, 2010.

Source: Dissertation Abstracts International, Volume: 71-12, Section: B, page: 7344.

Adviser: David Allman.

Local Notes:

School code: 0175.

ISBN:

9781124325040

Access Restriction:

Restricted for use by site license.