Regulation of CCR5 use on primary CD4+ lymphocytes by R5X4 HIV-1 / Lamorris M. Loftin.

Format:

Book

Manuscript

Thesis/Dissertation

Author/Creator:

Loftin, Lamorris M.

Contributor:

Collman, Ronald G., advisor.

University of Pennsylvania.

Language:

English

Subjects (All):

Penn dissertations--Cell and molecular biology.

Cell and molecular biology--Penn dissertations.

Cell and Molecular Biology.

Academic Dissertations as Topic.

Medical Subjects:

Cell and Molecular Biology.

Academic Dissertations as Topic.

Local Subjects:

Penn dissertations--Cell and molecular biology.

Cell and molecular biology--Penn dissertations.

Physical Description:

vii, 130 pages : illustrations (some color) ; 29 cm

Production:

2010.

Summary:

HIV-1 strains that use CCR5 predominate after transmission and during the asymptomatic period of disease. However, in up to half of infected people, variants that use CXCR4 emerge, coincident with accelerated disease progression. The earliest CXCR4 using strains to appear, called R5X4 viruses, usually retain CCR5 use. Prototype R5X4 HIV-1 isolates infect macrophages using CCR5 and CXCR4, but CD4+ lymphocyte infection by these viruses is mediated predominantly by CXCR4. Here, we sought to identify obstacles to CCR5 use on CD4+ lymphocytes by R5X4 HIV-1. Using a panel of R5X4 Envs we found that, although CXCR4 was the predominant coreceptor used to infect CD4+ lymphocytes, there was a spectrum of CCR5 use. Greater CCR5 use on lymphocytes correlated with relative resistance to inhibition by CCR5 mAbs and small molecule antagonists in CCR5+ indicator cells. Increasing CCR5 expression on primary lymphocytes through cytokine stimulation or lentiviral transduction increased the proportion of entry mediated by CCR5 for all R5X4 isolates except 89.6. Env dependence on CCR5 density was then evaluated using a cell line in which levels of CD4 and CCR5 could be independently regulated. At non-limiting CD4 levels, strains with greater lymphocyte CCR5 use were better able to exploit limiting levels of CCR5, whereas those that used lymphocyte CCR5 poorly were more sensitive to reductions in CCR5 levels. Evaluation of the V3 sequences of the R5X4 viruses using algorithms that predict viral phenotype indicated an association between greater CCR5 use on lymphocytes and a predicted non-syncytium inducing phenotype. Introduction of an R306S mutation in the Env V3 domain of 89.6 enhanced its ability to use CCR5 at low levels and switched its preference to CCR5 for lymphocyte entry. Residue R306 in the 89.6 and C2-16 Envs was also associated with greater dependence on the amino terminus of CCR5 for infection. Thus, lymphocyte CCR5 is used by some R5X4 strains to a variable degree, low CCR5 expression coupled with inefficient Env-CCR5 interactions are the principal obstacles to lymphocyte CCR5 use by R5X4 HIV-1, and the spectrum of lymphocyte CCR5 use by R5X4 isolates is determined by variation in Env-CCR5 interactions.

Notes:

Adviser: Ronald G. Collman.

Thesis (Ph.D. in Cell and Molecular Biology) -- University of Pennsylvania, 2010.

Includes bibliographical references.