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Platelets mediate separation of blood and lymphatic vessels.
- Format:
- Book
- Thesis/Dissertation
- Author/Creator:
- Bertozzi, Cara.
- Language:
- English
- Subjects (All):
- Molecular biology.
- 0307.
- Penn dissertations--Cell and molecular biology.
- Cell and molecular biology--Penn dissertations.
- Local Subjects:
- Penn dissertations--Cell and molecular biology.
- Cell and molecular biology--Penn dissertations.
- 0307.
- Physical Description:
- 119 pages
- Contained In:
- Dissertation Abstracts International 71-07B.
- System Details:
- Mode of access: World Wide Web.
- text file
- Summary:
- Mammals circulate blood and maintain tissue fluid balance through distinct blood and lymphatic vascular networks. Separation of blood and lymphatic vessels requires the hematopoietic signaling proteins SYK and SLP-76, but how blood cells perform this function is not known. We have used genetic fate-mapping studies to exclude direct hematopoietic cell contribution to vascular endothelium during this process. We find that Podoplanin (PDPN), a cell surface protein expressed by lymphatic but not blood vessel endothelium, is required in a common pathway with SLP-76 for separation of the blood and lymphatic vasculatures. Loss of signaling by CLEC-2, a PDPN receptor known to activate SYK and SLP-76 signaling, confers blood-lymphatic vascular mixing identical to that in animals lacking PDPN, SYK or SLP-76. CLEC-2 is expressed selectively by platelets, and conditional deletion of Slp-76 in platelets reproduces this vascular mixing phenotype. Platelets are activated and form aggregates on lymphatic endothelium during blood flow ex vivo, and at blood-lymphatic separation points in the developing mouse vasculature. These studies reveal a previously unappreciated role for platelets that respond to lymphatic endothelial cells and mediate blood-lymphatic vascular separation during embryonic development.
- Notes:
- Thesis (Ph.D. in Cell and Molecular Biology) -- University of Pennsylvania, 2010.
- Source: Dissertation Abstracts International, Volume: 71-07, Section: B, page: .
- Adviser: Mark L. Kahn.
- Local Notes:
- School code: 0175.
- ISBN:
- 9781124064024
- Access Restriction:
- Restricted for use by site license.
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