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Editing of antibody genes: A study in genetically simplified lines of mice.
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View online- Format:
- Book
- Thesis/Dissertation
- Author/Creator:
- Luning Prak, Eline Tjetske.
- Language:
- English
- Subjects (All):
- Immunology.
- Molecular biology.
- 0307.
- 0982.
- Penn dissertations--Immunology.
- Immunology--Penn dissertations.
- Local Subjects:
- Penn dissertations--Immunology.
- Immunology--Penn dissertations.
- 0307.
- 0982.
- Physical Description:
- 222 pages
- Contained In:
- Dissertation Abstracts International 57-04B.
- System Details:
- Mode of access: World Wide Web.
- text file
- Summary:
- Antibody gene rearrangements can continue despite the presence of productively rearranged heavy and light chain genes. The function(s) of these ongoing antibody gene rearrangements is unknown, although it has been proposed that continued rearrangement allows B cells with defective or autoreactive receptors to edit those receptors. To study the role of editing in rescuing B cells with dysfunctional receptors, animals with simplified immunoglobulin light chain genes were constructed and characterized: $\kappa$ deficient animals were used to assess the extent of ongoing rearrangement of $\kappa$ and $\lambda$ light chains. Animals with only one functional $\kappa$ L-chain allele (kdel/wt) were used to study editing by successive $\kappa$ L-chain rearrangement (leapfrogging). Consistent with leapfrogging, a bias towards distal J$\kappa$ rearrangements was observed in kdel/wt B cells. Southern blotting and PCR analysis confirmed the presence of multiple $\kappa$ rearrangements in some kdel/wt clones. To study editing of $\lambda$ light chains, rearrangements were characterized in kde/kdel mice. Some kdel/kdel B cells had multiple, productive $\lambda$ rearrangements, suggestive of editing by light chain competition for heavy chain pairing.
- Notes:
- Thesis (Ph.D. in Immunology) -- University of Pennsylvania, 1996.
- Source: Dissertation Abstracts International, Volume: 57-04, Section: B, page: 2464.
- Supervisor: Martin Weigert.
- Local Notes:
- School code: 0175.
- Access Restriction:
- Restricted for use by site license.
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