Aptamers in bioanalysis / edited by Marco Mascini.

Format:

Book

Contributor:

Mascini, Marco.

Language:

English

Subjects (All):

Oligonucleotides.

Medical Subjects:

Oligonucleotides.

Physical Description:

xvii, 313 pages, 12 unnumbered pages of plates : illustrations (some color) ; 24 cm

Place of Publication:

Oxford ; Hoboken, NJ : Wiley-Blackwell, [2009]

Summary:

This book details bioanalytical technologies and methods that have been developed using aptamers in analytical, medical, environmental, and food science applications. Because aptamers have some advantages over their rival antibodies, this is a rapidly emerging field with vast potential in varied disciplines. After an introduction to aptamers, aptamer targets, and their general uses, Aptamers in Bioanalysis:

Discusses different applications, with particular attention to the comparison between aptamer-based biosensors and methods versus the corresponding immunosensors

Includes examples of aptamer-based diagnostic techniques such as whole-cell protein profiling (proteomics) and medical diagnostics for the distinction of diseased versus healthy states

Includes chapters written by leading experts in their fields

With an overview of proven bioanalytical technologies and methods and numerous references for further study, this is a core reference for analytical chemists, electrochemists, pharmaceutical/medicinal chemists, and biotechnologists.

Contents:

I Introduction

1 Aptamers: Ligands for all Reasons / Jean-Jacques Toulme, Jean-Pierre Daguer, Eric Dausse 3

1.1 Introduction 3

1.2 The Power of Selection and Aptamer Refinement 5

1.3 The Chemistry Drives the Shape 7

1.4 Aptaregulators 11

1.5 Aptasensors 15

1.6 Prospects 18

References 20

2 Selex and Its Recent Optimizations / Beate Strehlitz, Regina Stoltenburg 31

2.1 Introduction 31

2.2 Aptamers and Their Selection by SELEX 32

2.3 Modifications of SELEX Technology 35

2.4 Advantages and Limitations of Aptamers and Their Selection Technology 41

2.5 Applications of Aptamers Being Developed for the Market 43

2.6 Future Perspectives 45

References 50

II Biosensors

3 Electrochemical Aptasensors / Itamar Willner, Maya Zayats 63

3.1 Introduction 63

3.2 Electrochemical Aptasensor Based on Redox-Active Aptamer Monolayers Linked to Electrodes 66

3.3 Enzyme-Based Amplified Electrochemical Aptasensors 69

3.4 Amplified Electrochemical Aptasensors Based on Nanoparticles 72

3.5 Label-Free Electrochemical Aptasensors 75

3.6 Field-Effect Transistor-Based Aptasensors 78

3.7 Conclusions and Perspectives 81

References 81

4 Aptamers: Hybrids between Nature and Technology / Moritz K. Beissenhirtz, Eik Leupold, Walter Stocklein, Ulla Wollenberger, Oliver Panke, Fred Lisdat, Frieder W. Scheller 87

4.1 Introduction 87

4.2 Specific Features of Aptamers 88

4.3 Electrochemical Detection of Nucleic Acids 88

4.4 Cytochrome c Binding by Aptamers 90

4.5 DNA Machines and Aptamers 92

References 98

5 Detection of Protein-Aptamer Interactions by Means of Electrochemical Indicators and Transverse Shear Mode Method / Tibor Hianik 101

5.1 Introduction 101

5.2 Immobilization of Aptamers on a Solid Support 102

5.3 Detection of Aptamer-Ligand Interactions 104

5.3.1 Electrochemical Methods 105

5.3.2 Acoustic Methods 117

5.4 Conclusions 124

References 125

6 Biosensors Using the Aptameric Enzyme Subunit: The use of Aptamers in the Allosteric Control of Enzymes / Kazunori Ikebukuro, Wataru Yoshida, Koji Sode 129

6.1 Aptamers as Molecular Recognition Elements of Biosensors 129

6.1.1 Comparing Aptamers to Antibodies 129

6.1.2 Signaling Aptamers 131

6.2 Homogeneous Sensing 133

6.2.1 Biosensor Systems That Do Not Require Bound-Free Separation 133

6.2.2 Aptameric Enzyme Subunit 133

6.3 Evolution-mimicking Algorithm for the Improvement of Aptamers 136

References 137

7 Nanomaterial-Based Label-Free Aptasensors / Kagan Kerman, Eiichi Tamiya 139

7.1 Introduction 139

7.2 Label-Free Electrochemical Aptasensors 139

7.3 Field-Effect Transistor-Based Aptasensors 143

7.4 Label-Free Aptasensors Based on Localized Surface Plasmon Resonance 147

7.5 Forthcoming Challenges and Concluding Remarks 151

References 151

8 Aptamer-Based Bioanalytical Assays: Amplification Strategies / Sara Tombelli, Maria Minunni, Marco Mascini 159

8.1 Introduction 159

8.2 Bioanalytical Assays Based on Aptamer-Functionalized Nanoparticles 160

8.3 Aptamers and Quantum Dot-Based Assays 164

8.4 Aptazymes and Aptamer-Based Machines 168

8.5 Polymerase Chain Reaction as an Amplification Method in Aptamer-Based Assays 173

8.6 Conclusions 176

References 177

III Applications

9 Kinetic Capillary Electrophoresis for Selection, Characterization, and Analytical Utilization of Aptamers / Sergey N. Krylov 183

9.1 Introduction 183

9.1.1 Kinetic Capillary Electrophoresis 183

9.1.2 The Concept of NECEEM and ECEEM 185

9.2 Selection of Aptamers Using KCE Methods for Partitioning and Affinity Control 188

9.2.1 NECEEM-Based Selection of Aptamers 188

9.2.2 ECEEM-Based Selection of Aptamers 197

9.2.3 Optimization of PCR 198

9.2.4 Future of KCE Methods for Aptamer Selection 200

9.3 Measurements of Binding Parameters of Target-Aptamer Interaction by KCE Methods 200

9.3.1 Foundation 200

9.3.2 Temperature Control Inside the Capillary 202

9.3.3 Examples 203

9.4 Quantitative Affinity Analysis of a Target Using Aptamer as an Affinity Probe 205

9.4.1 Foundation 205

9.4.2 Example 208

9.5 Conclusions 209

References 210

10 Aptamers for Separation of Enantiomers / Corinne Ravelet, Eric Peyrin 213

10.1 Introduction 213

10.2 Generation and Properties of Enantioselective Aptamers 214

10.3 Immobilized Aptamers for Enantiomeric Separation by Liquid Chromatography 215

10.3.1 Stationary-Phase Preparation and Column Packing 216

10.3.2 DNA Aptamer-Based CSPs 216

10.3.3 RNA Aptamer-Based CSPs and the Mirror-Image Strategy 217

10.3.4 Class-Specific Aptamer-Based CSPs 219

10.4 Aptamers for Analysis of Enantiomers by Capillary Electrophoresis 221

10.4.1 Aptamers as Chiral Additives in the Background Electrolyte for CE Enantiomeric Separation 221

10.4.2 Aptamers for the Design of an Affinity CE-Based Enantioselective Competitive Assay 223

10.5 Conclusions 226

References 226

11 Aptamer-Modified Surfaces for Affinity Capture and Detection of Proteins in Capillary Electrophoresis and Maldi-Mass Spectrometry / Linda B. McGown 229

11.1 Introduction 229

11.2 Aptamer-Modified Capillaries in Affinity Capillary Electrophoresis 230

11.3 Aptamer-Modified Surfaces for Affinity MALDI-MS 232

11.3.1 Overview 232

11.3.2 Affinity MALDI-MS of Thrombin 233

11.3.3 Affinity MALDI-MS of IgE 235

11.3.4 Summary 242

11.4 Beyond Aptamers: Genome-Inspired DNA Binding Ligands 242

References 248

12 Strategy for use of Smart Routes to Prepare Label-Free Aptasensors for Bioassay Using Different Techniques / Bingling Li, Hui Wei, Shaojun Dong 251

12.1 Introduction 251

12.2 Electrochemical Aptasensors 254

12.2.1 POSOALF Mode 254

12.2.2 PFSOALF Mode 257

12.2.3 Electrochemical Impedimetric Aptasensors 257

12.2.4 Electrochemical Aptasensors with Nonlabeled Redox Probes 263

12.3 Fluorescent Molecular Switches 265

12.3.1 POSFALF Mode 266

12.3.2 PFSFALF Mode 268

12.4 Colorimetry 272

12.4.1 POSFALF Mode 273

12.4.2 PFSFALF Mode 274

12.5 Hemin-Aptamer DNAzyme-Based Aptasensor 281

12.6 Liquid Chromatography, Electrochromatography, and Capillary Electrophoresis Applications 284

12.7 Other Aptasensors 290

12.8 Conclusions 290

References 290.

Notes:

Includes bibliographical references and index.

ISBN:

9780470148303

0470148306

OCLC:

226355790