Matrix metalloproteinase protocols / edited by Ian M. Clark ; consulting editors, David A. Young, Andrew D. Rowan.

Format:

Book

Contributor:

Clark, Ian M.

Young, David A. (David Alan), 1970-

Rowan, Andrew D.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 622.

Methods in molecular biology, 1064-3745 ; 622

Language:

English

Subjects (All):

Metalloproteinases.

Proteolytic enzymes.

Biochemistry--Technique.

Biochemistry.

Matrix Metalloproteinases.

Extracellular Matrix Proteins.

Medical Subjects:

Matrix Metalloproteinases.

Extracellular Matrix Proteins.

Genre:

Laboratory manuals.

Physical Description:

xii, 492 pages : illustrations (some color) ; 27 cm.

Edition:

Second edition.

Place of Publication:

New York, N.Y. : Humana Press, [2010]

Contents:

Metalloproteases and the degradome / Alejandro P. Ugalde ... [et al.]

Mouse models of MMP and TIMP function / Sean E. Gill ... [et al.]

Expression of recombinant MMP-28 in mammalian cells / Ursula R. Rodgers and Ian M. Clark

Expression of recombinant matrix metalloproteinases in Escherichia coli / L. Jack Windsor and Darin L. Steele

Expression of recombinant ADAMTS in insect cells / Gavin C. Jones, Mireille N. Vankemmelbeke and David J. Buttle

Expression and purification of membrane-type MMPs / Jing Nie and Duanqing Pei

Refolding of TIMP-2 from Escherichia coli inclusion bodies / Richard A. Williamson

Purification of MMPs and TIMPs / Ken-ichi Shimokawa and Hideaki Nagase

Real-time PCR expression profiling of MMPs and TIMPs / Caroline J. Pennington and Dylan R. Edwards

Analysis of the degradome with the CLIP-CHIP microarray / Reinhild Kappelhoff, Ulrich auf dem Keller, and Christopher M. Overall

In situ hybridization for metalloproteinases and their inhibitors / Tiina L. Hurskainen and Suneel S. Apte

Immunohistochemistry of MMPs and TIMPs / Yasunori Okada

Single nucleotide polymorphism genotyping in MMP genes : the 5' nuclease assay / Ross Laxton and Shu Ye

Methods for studying activation of matrix metalloproteinases / Vera Knäuper and Gillian Murphy

Assay of matrix metalloproteinases against matrix substrates / Tim E. Cawston, Rachel L. Lakey, and Andrew D. Rowan

Zymography and reverse zymography for detecting MMPs and TIMPs / Susan P. Hawkes, Hongxia Li, and Gary T. Taniguchi, with contributions from Marc A. Lafleur

In situ zymography / Sarah J. George and Jason L. Johnson

Near-infrared optical proteolytic beacons for in vivo imaging of matrix metalloproteinase activity / J. Oliver McIntyre, Randy L. Scherer, and Lynn M. Matrisian

Neoepitope antibodies against MMP-cleaved and aggrecanase-cleaved aggrecan / Amanda J. Fosang ... [et al.]

In vitro model of cartilage degradation / Wang Hui and Tim E. Cawston

Immunoassays for collagenase-mediated cleavage of type I and II collagens / R. Clark Billinghurst, Mirela Ionescu, and A. Robin Poole

Collagen degradation assays / Anthony P. Hollander

Analysis of MMP-dependent cell migration and invasion / Ralf Palmisano and Yoshifumi Itoh

Using fluorogenic peptide substrates to assay matrix metalloproteinases / Gregg B. Fields

Kinetic analysis of the inhibition of matrix metalloproteinases : lessons from the study of tissue inhibitors of metalloproteinases / Frances Willenbrock, Daniel A. Thomas, and Augustin Amour

Identification of cellular MMP substrates using quantitative proteomics : isotope-coded affinity tags (ICAT) and isobaric tags for relative and absolute quantification (iTRAQ) / Georgina S. Butler ... [et al.]

Mechanism-based profiling of MMPs / Jed F. Fisher and Shahriar Mobashery.

Notes:

"Springer protocols"--Cover.

Previous ed.: 2001.

Includes bibliographical references and index.

ISBN:

9781603272988

1603272984

OCLC:

467769866