Rat genomics : methods and protocols / edited by Ignacio Anegon.

Format:

Book

Contributor:

Anegon, Ignacio.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 597.

Methods in molecular biology, 1064-3745 ; 597

Language:

English

Subjects (All):

Rats--Molecular genetics.

Rats.

Genomics.

Rats--Functional genomics.

Functional genomics.

Rats--genetics.

Molecular genetics.

Medical Subjects:

Genomics.

Rats--genetics.

Genre:

Laboratory manuals.

Physical Description:

xvi, 461 pages : illustrations (some color) ; 27 cm.

Place of Publication:

New York : Humana Press, Springer Science+Business Media, [2010]

Contents:

The Rat: a model used in biomedical research / Howard J. Jacob

Genetic mapping and positional cloning / Timothy J. Aitman, Enrico Petretto, Jacques Behmoaras

Sequencing of the rat genome and databases / Elizabeth A. Worthey, Alexander J. Stoddard, Howard J. Jacob

Design of expression cassettes for the generation of transgenic animals (including insulators) / Louis-Marie Houdebine

Inducible and conditional promoter systems to generate transgenic animals / Yoji Hakamata, Eiji Kobayashi

Generation of transgenic rats by microinjection of short DNA fragments / Séverine Ménoret, Séverine Remy, Claire Usal, Laurent Tesson, Ignacio Anegon

Generation of transgenic rats using YAC and BAC DNA constructs / Ri-ichi Takahashi, Masatsugu Ueda

The Use of lentiviral vectors to obtain transgenic rats / Séverine Remy, Tuan Huy Nguyen, Séverine Ménoret, Laurent Tesson, Claire Usal, Ignacio Anegon

Generation of transgenic rats by ooplasmic injection of sperm cells exposed to exogenous DNA / Masumi Hirabayashi, Shinichi Hochi

Procedures for somatic cell nuclear transfer in the rat / Jean Cozzi, Eryao Wang, Christelle Jacquet, Alexandre Fraichard, Yacine Cherifi, Qi Zhou

ENU mutagenesis to generate genetically modified rat models / Ruben Boxtel, Michael N. Gould, Edwin Cuppen, Bart M.G. Smits

Establishment of embryonic stem cells from rat blastocysts / Masaki Kawamata, Takahiro Ochiya

Derivation, culture, and in vivo developmental capacity of embryonic cell lines from rat blastocysts / Simon-Pierre Demers, Lawrence C. Smith

Interference RNA for in vivo knock-down of gene expression or genome-wide screening using shRNA / Silvère Petit, Kader Thiam

Generation of gene-specific mutated rats using zinc-finger nucleases / Aron M. Geurts, Gregory J. Cost, Séverine Rémy, Xiaoxia Cui, Laurent Tesson, Claire Usal, Séverine Ménoret, Howard J. Jacob, Ignacio Anegon, Roland Buelow

Application of microarray-based analysis of gene expression in the field of toxicogenomics / Nan Mei, James C. Fuscoe, Edward K. Lobenhofer, Lei Guo

Generation of congenic and consomic rat strains / Dominique Lagrange, Gilbert J Fournié

Generation of rat "supersonic" congenic/conplastic strains using superovulation and embryo transfer / Vladimír Landa, Václav Zídek, Michal Pravenec

Analysis by quantitative PCR of zygosity in genetically modified organisms / Laurent Tesson, Séverine Rémy, Séverine Ménoret, Claire Usal, Ignacio Anegon

A Restriction enzyme-PCR-based technique to determine transgene insertion sites / Elizabeth C. Bryda, Beth A. Bauer

Cryopreservation and orthotopic transplantation of rat ovaries / Martina Dorsch, Dirk Wedekind

Techniques for in vitro and in vivo fertilization in the rat / Naomi Kashiwazaki, Yasunari Seita, Akiko Takizawa, Naoki Maedomari, Junya Ito, Tadao Serikawa

Rat strain repositories / Birger Voigt

Neurobehavioral tests in rat models of degenerative brain diseases / Yvonne K. Urbach, Felix J. Bode, Huu Phuc Nguyen, Olaf Riess, Stephan von Hörsten

Rat genomics applied to psychiatric research / Marie-Pierre Moisan, André Ramos

Genomics studies of immune-mediated diseases using the BN-LEW rat model / Isabelle Bernard, Gilbert J. Fournié, Abdelhadi Saoudi

Rat models of cardiovascular diseases / Michael Bader

Use of rat genomics for investigating the metabolic Syndrome / Michal Pravenec

Genomic research in rat models of kidney disease / Yoram Yagil, Chana Yagil

Cancer research in rat models / Claude Szpirer

Notes:

"Springer protocols"--Cover.

Includes bibliographical references and index.

ISBN:

9781603273886

1603273883

1603273891

9781603273893

OCLC:

428029172