Drug-DNA interaction protocols / edited by Keith R. Fox.

Format:

Book

Contributor:

Fox, Keith R.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 613.

Methods in molecular biology, 1064-3745 ; 613

Language:

English

Subjects (All):

DNA-drug interactions.

DNA--drug effects.

DNA Footprinting--methods.

Polymerase Chain Reaction--methods.

DNA Probes.

DNA-Binding Proteins.

Pharmaceutical Preparations--metabolism.

DNA--metabolism.

DNA--chemistry.

Medical Subjects:

DNA--drug effects.

DNA Footprinting--methods.

Polymerase Chain Reaction--methods.

DNA Probes.

DNA-Binding Proteins.

Pharmaceutical Preparations--metabolism.

DNA--metabolism.

DNA--chemistry.

Genre:

Laboratory manuals.

Physical Description:

xi, 318 pages : illustrations (some color) ; 26 cm.

Edition:

Second edition.

Place of Publication:

New York, N.Y. : Humana Press, Springer Science+Business Media, [2010]

Contents:

1. Quantitative analysis of small molecule-nucleic acid interactions with a biosensor surface and surface plasmon resonance detection / Yang Liu and W. David Wilson

2. Thermal melting studies of ligand DNA interactions / Aurore Guédin, Laurent Lacroix, and Jean-Louis Mergny

3. Circular and linear dichroism of drug-DNA systems / Alison Rodger

4. Drug binding to DNA-RNA hybrid structures / Richard T. Wheelhouse and Jonathan B. Chaires

5. Quantification of binding data using capillary electrophoresis / Fitsumbirhan Araya, Graham G. Skellern, and Roger D. Waigh

6. Determination of equilibrium association constants of ligand-DNA complexes by electrospray mass spectrometry / Valérie Gabelica

7. Detection of adriamycin-DNA adducts by accelerator mass spectrometry / Kate Coldwell ... [et al.]

8. Molecular modelling methods to quantitate drug-DNA interactions / Hao Wang and Charles A. Laughton

9. Application of anomalous diffraction methods to the study of DNA and DNA-complexes / Derrick Watkins ... [et al.]

10. DNase I footprinting / Antonia S. Cardew and Keith R. Fox

11. Methods to characterize the effect of DNA-modifying compounds on nucleosomal DNA / Vidya Subramanian ... [et al.]

12. REPSA: combinatorial approach for identifying preferred drug-DNA binding sequences / Michael W. Van Dyke

13. In vitro transcription assay for resolution of drug-DNA interactions at defined DNA sequences / Benny J. Evison, Don. R. Phillips, and Suzanne M. Cutts

14. In virtro footprinting of promoter regions within supercoiled plasmid DNA / Daekyu Sun

15. Topoisomerase I-mediated DNA relaxation as a tool to study intercalation of small molecules and supercoiled DNA / Paul Peixoto ... [et al.]

16. A high-throughput assay for DNA topoisomerases and other enzymes based on DNA triplex formation / Matthew R. Burrell, Nicolas P. Burton and Anthony Maxwell

17. Measurement of DNA interstrand crosslinking in individual cells using the single cell gel electrophoresis (Comet) assay / Victoria J. Spanswick, Janet M. Hartley, and John A. Hartley

18. Measurement of DNA interstrand crosslinking in naked DNA using Gel-based methods / Konstantinous Kiakos, Janet M. Hartley, and John A. Hartley

19. An evaluation cascade for G-quadruplex telomere targeting agents in human cancer cells / Mekala Gunaratnam and Stephen Neidle.

Notes:

"Springer protocols"--Cover.

Includes bibliographical references and index.

ISBN:

9781603274173

1603274170

9781603274180

1603274189

OCLC:

503442364