Plant microRNAs : methods and protocols / edited by Blake C. Meyers, Pamela J. Green.

Format:

Book

Contributor:

Meyers, Blake C.

Green, Pamela J.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 592.

Methods in molecular biology, 1064-3745 ; 592

Language:

English

Subjects (All):

Small interfering RNA.

Plant molecular genetics--Research--Methodology.

Plant molecular genetics.

MicroRNAs--genetics.

Research.

Methodology.

Plants--genetics.

RNA, Small Interfering--genetics.

Medical Subjects:

MicroRNAs--genetics.

Plants--genetics.

RNA, Small Interfering--genetics.

Genre:

Laboratory manuals.

Physical Description:

xii, 267 pages : illustrations ; 27 cm.

Place of Publication:

New York, NY : Humana Press ; London : Springer [distributor], [2010]

Contents:

Piecing the puzzle together : genetic requirements for miRNA biogenesis in Arabidopsis thaliana / Zhixin Xie

Prediction of plant miRNA genes / Matthew W. Jones-Rhoades

Methods for isolation of total RNA to recover miRNAs and other small RNAs from diverse species / Monica Accerbi ... [et al.]

miRNA target prediction in plants / Noah Fahlgren and James C. Carrington

A method to discover phased siRNA loci / Michael J. Axtell

Directed gene silencing with artificial microRNAs / Rebecca Schwab ... [et al.]

Bioinformatics analysis of small RNAs in plants using next generation sequencing technologies / Kan Nobuta ... [et al.]

High-throughput approaches for miRNA expression analysis / Cheng Lu and Frédéric Souret

In situ detection of miRNAs using LNA probes / Zoltán Havelda

Analysis of miRNA modifications / Bin Yu and Xuemei Chen

MicroRNA promoter analysis / Molly Megraw and Artemis G. Hatzigeorgiou

Computational methods for comparative analysis of plant small RNAs / Gayathri Mahalingam and Blake C. Meyers

Biotic stress-associated microRNAs : identification, detection, regulation, and functional analysis / Florence Jay ... [et al.]

Abiotic stress-associated miRNAs : detection and functional analysis / Dong-Hoon Jeong ... [et al.]

Processing of miRNA precursors / Yukio Kurihara and Yuichiro Watanabe

Purification of Arabidopsis argonaute complexes and associated small RNAs / Yijun Qi and Shijun Mi

Transient assays for the analysis of miRNA processing and function / Felipe F. de Felippes and Detlef Weigel.

Notes:

"Springer protocols"--Cover.

Includes bibliographical references and index.

ISBN:

9781603270045

1603270043

OCLC:

262719622