Reverse chemical genetics : methods and protocols / edited by Hisashi Koga.

Format:

Book

Contributor:

Koga, Hisashi.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 577.

Methods in molecular biology, 1064-3745 ; 577

Language:

English

Subjects (All):

Biochemical genetics.

Combinatorial Chemistry Techniques--methods.

Genetic Techniques.

Molecular Biology--methods.

Medical Subjects:

Combinatorial Chemistry Techniques--methods.

Genetic Techniques.

Molecular Biology--methods.

Genre:

Laboratory manuals.

Physical Description:

xi, 272 pages : illustrations (some color) ; 27 cm.

Place of Publication:

New York, NY : Humana Press, [2009]

Summary:

Now a major approach in the identification of certain gene product functions, reverse chemical genetics allows the identification of target genes to precede phenotypic changes created by specific small molecules. In Reverse Chemical Genetics: Methods and Protocols, expert authors introduce fundamental resources, including databases for reverse chemical genetics, as well as the modification of these cDNA resources by the most recent molecular technologies, The book explores newly established methods in addition to potentially applicable techniques that make use of specific small molecules, plus functional assays utilizing these molecules, while focusing on methods that make feasible the functional discovery and comprehensive knowledge of particular target genes. As a volume in the highly successful Methods in Molecular Biology^trade; series, chapters contain introductions to their respective topics, list of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Reverse Chemical Genetics: Methods and Protocols promise to inspire researches to conduct their own experiments in order to progress this vital field of study.

Contents:

ORFeome cloning / Osamu Ohara

Systematic cloning of an ORFeome using the gateway system / Akihisa Matsuyama and Minoru Yoshida

High-throughput construction of ORF clones for production of the recombinant proteins / Hisashi Yamakawa

Computational overview of GPCR gene universe to support reverse chemical genomics study / Makiko Suwa and Yukiteru Ono

High-performance gene expression module analysis tool and its application to chemical toxicity data / Wataru Fujibuchi ... [et al.]

Human protein reference database and human proteinpedia as discovery tools for systems biology / T.S. Keshava Prasad, Kumaran Kandasamy, and Akhilesh Pandey

High-throughput production of the recombinant proteins expressed in Escherichia coli utilizing cDNA resources / Kiyo Shimada and Hisashi Koga

An insect cell-free system for recombinant protein expresion using cDNA resources / Takashi Suzuki ... [et al.]

Recombinant protein production by a kaiko-baculovirus system / Hidekazu Nagaya

Pulse-chase experiment for the analysis of protein stability in cultured mammalian cells by covalent fluorescent labeling of fusion proteins / Kei Yamaguchi ... [et al.]

Application of 2D-DIGE in cancer proteomics toward personalized medicine / Tadashi Kondo and Setsuo Hirohashi

Fully automated two-dimensional electrophoresis system for high-throughput protein analysis / Atsunori Hiratsuka and Kenji Yokoyama

Large-scale whole mount in situ hybridization of mouse embryos / Hirohito Shimizu, Kenta Uchibe, and Hiroshi Asahara

Identification of the specific binding proteins of bioactive small coumpound using affinity resins / Akito Tanaka

Protein microarrays : effective tools for the study of inflammatory diseases / Xiaobo Yu ... [et al.]

Preparation of highly sensitive protein array using reactive polymer / Toshifumi Shiroya ... [et al.]

High-throughput SPR biosensor / Motoki Kyo ... [et al.]

Drug discovery through functional screening in the Drosophila heart / Takeshi Akasaka and Karen Ocorr

Permeable cell assay : a method for high-throughput measurement of cellular ATP synthetic activity / Kiyotaka Y. Hara

Novel methodology for immobilization of biomolecules on the surface of a photoresponsible polymer containing azobenzene moiety / Osamu Watanabe.

Notes:

"Springer protocols"--Cover.

Includes bibliographical references and index.

ISBN:

9781607612315

1607612313

OCLC:

401153915