Bioluminescence : methods and protocols / edited by Preston B. Rich and Christelle Douillet.

Format:

Book

Contributor:

Rich, Preston B.

Douillet, Christelle D.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 574.

Methods in molecular biology, 1064-3745 ; 574

Language:

English

Subjects (All):

Bioluminescence assay.

Luminescent Measurements--methods.

Biosensing Techniques.

Medical Subjects:

Luminescent Measurements--methods.

Biosensing Techniques.

Physical Description:

xi, 267 pages : illustrations (some color) ; 27 cm.

Edition:

Second edition.

Place of Publication:

New York, NY : Humana Press, [2009]

Summary:

Through the study and application of bioluminescence, scientists have painstakingly harnessed a powerful tool that enables us to seek a deeper understanding of the complex mechanisms underpinning so many vital biologic systems. In this fully revised and updated second edition of Bioluminescence: Methods and Protocols, expert researchers contribute a readable and utilitarian compilation of the newest and most innovative techniques that have emerged in this rapidly expanding and progressively diverse field including methods to assess cell trafficking, protein-protein interactions, intracellular signaling, and apoptosis. Also opening up the possibility to visualize and quantify biological mechanisms in real-time and in vivo settings, the volume also describes the in vivo study of bacterial or viral infections, transplanted cells, stem cells proliferation, vascular flow, and tumors. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include brief introductions to their respective topics, lists of the necessary materials, equipment, and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Bioluminescence: Methods and Protocols, Second Edition provides protocols that are detailed enough to be followed and adapted by scientific teams who have no previous expertise in bioluminescence in order to foster the potentially numerous breakthroughs and new applications from basic to applied science and medicine that must continue to be developed.

Contents:

1 Luminescent Probes and Visualization of Bioluminescence / Elisa Michelini, Luca Cevenini, Laura Mezzanotte, Aldo Roda 1

2 Validation of Bioluminescent Imaging Techniques / John Virostko, E. Duco Jansen 15

3 Assessment of Extracellular ATP Concentrations / Lucia Seminario-Vidal, Eduardo R. Lazarowski, Seiko F. Okada 25

4 High-Throughput Quantitative Bioluminescence Imaging for Assessing Tumor Burden / Angelina Contero, Edmond Richer, Ana Gondim, Ralph P. Mason 37

5 Fluorescence Imaging of Tumors with "Smart" pH-Activatable Targeted Probes / Daisuke Asanuma, Hisataka Kobayashi, Tetsuo Nagano, Yasuteru Urano 47

6 Imaging Vasculature and Lymphatic Flow in Mice Using Quantum Dots / Byron Ballou, Lauren A. Ernst, Susan Andreko, James A. J. Fitzpatrick, B. Christoffer Lagerholm, Alan S. Waggoner, Marcel P. Bruchez 63

7 Bioluminescent Imaging of Transplanted Islets / Xiaojuan Chen, Dixon B. Kaufman 75

8 Bioluminescence Reporter Gene Imaging of Human Embryonic Stem Cell Survival, Proliferation, and Fate / Kitchener D. Wilson, Mei Huang, Joseph C. Wu 87

9 Detection of Apoptosis Using Cyclic Luciferase in Living Mammals / Akira Kanno, Yoshio Umezawa, Takeaki Ozawa 105

10 Noninvasive Bioluminescent Imaging of Infections / Javier S. Burgos 115

11 Real-Time Bioluminescence Imaging of Viral Pathogenesis / Kathryn E. Luker, Gary D. Luker 125

12 Bioluminescent Monitoring of In Vivo Colonization and Clearance Dynamics by Light-Emitting Bacteria / Siouxsie Wiles, Brian D. Robertson, Gad Frankel, Angela Kerton 137

13 Quantitative In Vivo Imaging of Non-viral-Mediated Gene Expression and RNAi-Mediated Knockdown / Garrett R. Rettig, Kevin G. Rice 155

14 Analysis of Protein-Protein Interactions Using Bioluminescence Resonance Energy Transfer / Kevin D.G. Pfleger 173

15 Bioluminescent Imaging of MAPK Function with Intein-Mediated Reporter Gene Assay / Akira Kanno, Takeaki Ozawa, Yoshio Umezawa 185

16 Bioluminescence Analysis of Smad-Dependent TGF-β Signaling in Live Mice / Jian Luo, Tony Wyss-Coray 193

17 Bioluminescence Imaging of Calcium Oscillations Inside Intracellular Organelles / Carlos Villalobos, María Teresa Alonso, Javier García-Sancho 203

18 Novel Tools for Use in Bioluminescence Resonance Energy Transfer (BRET) Assays / Mélanie Robitaille, Isabelle Héroux, Alessandra Baragli, Terence E. Hébert 215

19 PIN-G Reporter for Imaging and Defining Trafficking Signals in Membrane Proteins / Lynn Mckeown, Vicky C. Jones, Owen T. Jones 235

20 Imaging β-Galactosidase Activity In Vivo Using Sequential Reporter-Enzyme Luminescence / Georges von Degenfeld, Tom S. Wehrman, Helen M. Blau 249.

Notes:

"Springer protocols"--Cover.

Includes bibliographical references and index.

ISBN:

9781603273206

OCLC:

369132732