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Type 2 diabetes : methods and protocols / edited by Claire Stocker.
Holman Biotech Commons QH506 .M45 v.560 2009
Available
Holman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
Available
- Format:
- Book
- Series:
- Methods in molecular biology 1064-3745 ; v. 560.
- Methods in molecular biology ; 560
- Language:
- English
- Subjects (All):
- Type 2 diabetes--Laboratory manuals.
- Type 2 diabetes--Susceptibility--Research.
- Type 2 diabetes--Research.
- Diabetes Mellitus, Type 2.
- Diabetes Mellitus, Type 2--genetics.
- Diabetes Mellitus, Type 2--physiopathology.
- Type 2 diabetes.
- Research.
- Medical Subjects:
- Diabetes Mellitus, Type 2.
- Diabetes Mellitus, Type 2--genetics.
- Diabetes Mellitus, Type 2--physiopathology.
- Genre:
- Laboratory manuals.
- Physical Description:
- x, 278 pages : illustrations (some color) ; 26 cm.
- Place of Publication:
- Totowa, N.J. ; London : Humana, 2009.
- Summary:
- Diabetes is now reaching epidemic proportions, and the associated complications of this disease can be disabling and even life-threatening. In Type 2 Diabetes: Methods and Protocols, leading investigators provide up-to-date explanations of commonly used laboratory protocols used in diabetes research. Covering the commonly described in vivo and in vitro model systems, the volume ultimately leads to an overall view of how cellular dysfunction and degeneration leads to susceptibility and diabetes disease progression. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and expert notes on troubleshooting and avoiding known pitfalls.
- Comprehensive and cutting-edge, Type 2 Diabetes: Methods and Protocols offers succinct, proven techniques to aid research scientists and clinicians in continuing the study of this debilitating disease.
- Contents:
- 1 Selecting the "Right" Mouse Model for Metabolic Syndrome and Type 2 Diabetes Research / Edward H. Leiter 1
- 2 Nutritional Models of Type 2 Diabetes Mellitus / Beverly Sara Mühlhausler 19
- 3 The Isolation and Purification of Rodent Pancreatic Islets of Langerhans / Jacqueline F. O'Dowd 37
- 4 The Measurement of Insulin Secretion from Isolated Rodent Islets of Langerhans / Anna L. Nolan, Jacqueline F. O'Dowd 43
- 5 The Incubation and Monitoring of Cell Viability in Primary Rat Islets of Langerhans and Pancreatic β-Cell Lines / Noel G. Morgan, Eleftheria Diakogiannaki, Mark A. Russell 53
- 6 In Vitro Culture of Isolated Islets of Langerhans: Analysis of Function / Anna L. Nolan 65
- 7 Single-Cell RT-PCR Identification of Genes Expressed by Human Islet Endocrine Cells / Dany Muller, Peter M. Jones, Shanta J. Persaud 73
- 8 Laser Capture Microdissection of Human Pancreatic β-Cells and RNA Preparation for Gene Expression Profiling / Lorella Marselli, Dennis C. Sgroi, Susan Bonner-Weir, Gordon C. Weir 87
- 9 In Vitro Transdifferentiation of Human Hepatoma Cells into Pancreatic-Like Cells / Wan-Chun Li 99
- 10 The Measurement of GLUT4 Translocation in 3T3-L1 Adipocytes / Nickey Konstantopoulos, Juan Carlos Molero-Navajas 111
- 11 Pancreatic Remodeling: Beta-Cell Apoptosis, Proliferation and Neogenesis, and the Measurement of Beta-Cell Mass and of Individual Beta-Cell Size / Eduard Montanya, Noèlia Téllez 137
- 12 Morphology of Pancreatic Islets: A Time Course of Pre-diabetes in Zucker Fatty Rats / Petra Augstein, Eckhard Salzsieder 159
- 13 Fluorescent Immunohistochemistry and In Situ Hybridization Analysis of Pancreas / Xiuli Wang, Shundi Ge, Gay M. Crooks 191
- 14 The Measurement of Insulin Secretion Using Pancreas Perfusion in the Rodent / Edward T. Wargent 203
- 15 Hyperinsulinemic-Euglycemic Clamp to Assess Insulin Sensitivity In Vivo / Jason K. Kim 221
- 16 Gene Expression Analysis in Diabetes Research / Peter White, Klaus H. Kaestner 239
- 17 Gene Expression Mining in Type 2 Diabetes Research / Donald R. Dunbar 263.
- Notes:
- "Springer protocols"--Cover.
- Includes bibliographical references and index.
- ISBN:
- 9781934115152
- 1934115150
- 9781597454483
- 1597454486
- OCLC:
- 319204986
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