Cell-based assays for high-throughput screening : methods and protocols / edited by Paul A. Clemons, Nicola J. Tolliday, and Bridget K. Wagner.

Format:

Book

Contributor:

Clemons, Paul A.

Tolliday, Nicola J.

Wagner, Bridget K.

Series:

Methods in molecular biology (Clifton, N.J.) 1064-3745 ; v. 486.

Methods in molecular biology, 1064-3745 ; v. 486

Language:

English

Subjects (All):

Drug Discovery.

Biological Assay.

Drug Evaluation, Preclinical.

High throughput screening (Drug development).

Biological assay.

Medical Subjects:

Drug Discovery.

Biological Assay.

Drug Evaluation, Preclinical.

Genre:

Laboratory manuals.

Physical Description:

x, 218 pages : illustrations ; 27 cm.

Place of Publication:

New York : Humana, [2009]

Summary:

As the use of high-throughput screening expands and creates more interest in the academic community, the need for detailed reference materials becomes ever more pressing. Cell-Based Assays for High-Throughput Screening: Methods and Protocols aims to fill an important part of this need by providing an easily accessible reference volume for cell-based phenotypic screening. Leading researchers in the field contribute state-of-the-art methods with actionable protocols covering four major areas of study: model biological systems, screening modalities and assay systems, detection technologies, and approaches to data analysis. Written in the highly successful Methods in Molecular BiologyTM series format, each chapter includes a brief introduction to the subject, lists of necessary materials and reagents, step-by-step laboratory protocols, and a Notes section detailing tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and easy-to-use, Cell-Based Assays for High-Throughput Screening: Methods and Protocols presents an overview of relevant approaches, enabling the direct application of existing methods to new discoveries while also inspiring researchers to approach their screening projects in a conceptually modular fashion, enhancing the power to discover through new combinations of existing approaches.

Contents:

1 Introduction: Cell-Based Assays for High-Throughput Screening / W. Frank An, Nicola J. Tolliday 1

2 High-Throughput Screening of Model Bacteria / Soumaya Zlitni, Jan E. Blanchard, Eric D. Brown 13

3 Cell-Based Assays to Probe the ERK MAP Kinase Pathway in Endothelial Cells / Michael R. Wyler, Deborah H. Smith, Eftihia Cayanis, Udo Toebben, Nathalie Aulner, Thomas Mayer 29

4 Large-Scale Small-Molecule Screen Using Zebrafish Embryos / Charles C. Hong 43

5 Whole-Animal High-Throughput Screens: The C. elegans Model / Eyleen J. O'Rourke, Annie L. Conery, Terence I. Moy 57

6 Whole-Organism Screening: Plants / April Agee, David Carter 77

7 Florescence-Based Assays / W. Frank An 97

8 Reporter Gene Assays / Andy M.F. Liu, David C. New, Rico K.H. Lo, Yung H. Wong 109

9 Screening for Chemical Inhibitors of Heterologous Proteins Expressed in Yeast Using a Simple Growth-Restoration Assay / Aruna D. Balgi, Michael Roberge 125

10 Assay for Isolation of Inhibitors of Her2-Kinase Expression / Gabriela Chiosis, Adam B. Keeton 139

11 High-Content Screening: Flow Cytometry Analysis / Bruce S. Edwards, Susan M. Young, Irena Ivnitsky-Steele, Richard D. Ye, Eric R. Prossnitz, Larry A. Sklar 151

12 High-Throughput Real-Time PCR for Detection of Gene-Expression Levels / Bridget K. Wagner, Zoltan Arany 167

13 Interpretation of Uniform-Well Readouts / Serene Josiah 177

14 Extracting Rich Information from Images / Anne E. Carpenter 193.

Notes:

"Springer protocols"--Cover.

Includes bibliographical references and index.

ISBN:

9781603275446

1603275444

9781603275453

1603275452

OCLC:

233788493