Transgenic wheat, barley and oats : production and characterization protocols / edited by Huw D. Jones and Peter R. Shewry.

Format:

Book

Contributor:

Jones, Huw D.

Shewry, P. R. (Peter R.)

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 478.

Methods in molecular biology, 1064-3745 ; 478

Language:

English

Subjects (All):

Transgenic plants.

Wheat--Genetics.

Wheat.

Barley--Genetics.

Barley.

Oats--Genetics.

Oats.

Plants, Genetically Modified.

Genetic Engineering--methods.

Triticum--genetics.

Hordeum--genetics.

Avena--genetics.

Medical Subjects:

Plants, Genetically Modified.

Genetic Engineering--methods.

Triticum--genetics.

Hordeum--genetics.

Avena--genetics.

Genre:

Laboratory manuals.

Physical Description:

x, 349 pages, 8 unnumbered pages of plates : illustrations (some color) ; 27 cm.

Place of Publication:

New York, NY : Humana Press, [2009]

Summary:

The understanding of the physical and genetic structure of plant genomes and the determination of phenotypes via interactions of coding and non-coding regions with the environment has quickly become the key to the future of plant breeding and agriculture. In Transgenic Wheat, Barley and Oats: Production and Characterization Protocols, expert investigators contribute the latest protocols for the transformation, regeneration and selection of these three species, using both biolistics and Agrobacterium tumefaciens, in order to fill the need for techniques and resources in this vital area of research. Covering such topics as in vitro Agrobacterium co-cultivation, the manipulation of gene expression, and GM risk assessment, this versatile collection focuses on the study of temperate small grain cereals while many of its readily reproducible procedures could be easily adapted to accommodate other cereals and plants. The in-depth chapters adhere to the highly successful Methods in Molecular Biology series format, featuring brief introductions to the subject, lists of the necessary materials and reagents, step-by-step laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Transgenic Wheat, Barley and Oats: Production and Characterization Protocols is an ideal guide for scientists pursuing this powerful, forward-thinking genetic strategy.

Contents:

Transgenic wheat, barley and oats : production and characterization / Paul A. Lazzeri and Huw D. Jones

Selection of transformed plants / Huw D. Jones and Caroline A. Sparks

Reporter genes / Alison Huttly

Biolistics transformation of wheat / Caroline A. Sparks and Huw D. Jones

Agrobacterium-mediated transformation of bread and durum wheat using freshly isolated immature embryos / Huixia Wu, Angela Doherty and Huw D. Jones

Floral transformation of wheat / Sujata Agarwal ... [et al.]

Highly efficient agrobacterium-mediated transformation of wheat via in planta inoculation / Thierry Risacher ... [et al.]

Barley transformation using biolistic techniques / Wendy A. Harwood and Mark A. Smedley

Barley transformation using agrobacterium-mediated techniques / Wendy A. Harwood ... [et al.]

Transformation of oats and its application to improving osmotic stress tolerance / Shahina B. Maqbool ... [et al.]

Promoter sequences for defining transgene expression / Huw D. Jones and Caroline A. Sparks

Down-regulation of gene expression by RNA-induced gene silencing / Silvia Travella and Beat Keller

Gene insertion patterns and sites / Philippe Vain and Vera Thole

Fluorescent in situ hybridization to detect transgene integration into plant genomes / Trude Schwarzacher

Establishing substantial equivalence : transcriptomics / María Marcela Baudo ... [et al.]

Establishing substantial equivalence : proteomics / Alison Lovegrove, Louise Salt, and Peter R. Shewry

Establishing substantial equivalence : metabolomics / Michael H. Beale, Jane L. Ward, and John M. Baker

Design and management of field trials of transgenic cereals / Zoltán Bedő, Mariann Rakszegi, and László Láng

GM risk assessment / Penny A.C. Sparrow

Transgenic wheat, barley and oats : future prospects / Jim M. Dunwell.

Notes:

"Springer protocols"--Cover.

Includes bibliographical references and index.

ISBN:

9781588299611

1588299619

OCLC:

227922736