Protein engineering / Caroline Köhrer, Uttam L. RajBhandary (eds.).

Format:

Book

Contributor:

Köhrer, Caroline.

RajBhandary, Uttam

Alumni and Friends Memorial Book Fund.

Series:

Nucleic acids and molecular biology 0933-1891 ; v. 22.

Nucleic acids and molecular biology, 0933-1891 ; 22

Language:

English

Subjects (All):

Protein engineering.

Protein Engineering.

RNA, Transfer--chemical synthesis.

Medical Subjects:

Protein Engineering.

RNA, Transfer--chemical synthesis.

Physical Description:

xi, 347 pages : illustrations.

Place of Publication:

Berlin : Springer, [2009]

Summary:

Site-specific mutagenesis of DNA, developed some thirty years ago, has proven to be one of the most important advances in biology. By allowing the site-specific replacement of any amino acid in a protein with one of the other nineteen amino acids, it ushered in the new era of "Protein Engineering". The field of protein engineering has, however, evolved rapidly since then and the last fifteen years have witnessed remarkable advances through the use of new chemical, biochemical and molecular biological tools towards the synthesis and manipulation of proteins. The chapters included in this book reflect the rapid evolution of protein engineering and its many applications in basic research, biotechnology, material sciences and therapy. This book will provide the reader with an introduction to state-of the-art concepts and methods and will be of use to anyone interested in the study of proteins, in academia as well as in industry.

Contents:

Understanding enzyme mechanism through protein chimeragenesis / N.M. Goodey and S.J. Benkovic

Chemical protein engineering : synthetic and semisynthetic peptides and proteins / L. Merkel, L. Moroder, and N. Budisa

Native chemical ligation: Semisynthesis of post-translationally modified proteins and biological probes / E. Vogel Taylor and B. Imperiali

Chemical methods for mimicking post-translational modifications / S.I. van Kasteren, P. Garnier and B.G. Davis

Noncanonical amino acids in protein science and engineering / K.E. Beatty and D.A. Tirrell

Fidelity mechanisms of the aminoacyl-tRNA synthetases / A.P. Mascarenhas ... [et al.]

Specialized components of the translational machinery for unnatural amino acid mutagenesis : tRNAs, aminoacyl-tRNA synthetases, and ribosomes / C. Köhrer and U.L. RajBhandary

In vivo studies of receptors and ion channels with unnatural amino acids / D.A. Dougherty

Synthesis of modified proteins using misacylated tRNAs / S.M. Hecht

Cell-free synthesis of proteins with unnatural amino acids. The pure system and expansion of the genetic code / I. Hirao, T. Kanamori and T. Ueda

Engineering nucleobases and polymerases for an expanded genetic alphabet / A.M. Leconte and F.E. Romesberg

Understanding membrane proteins. How to design inhibitors of transmembrane protein-protein interactions / J.S. Slusky, H. Yin and W.F. DeGrado.

Notes:

Includes bibliographic references and index.

Local Notes:

Acquired for the Penn Libraries with assistance from the Alumni and Friends Memorial Book Fund.

ISBN:

9783540709374

3540709371

9783540709411

354070941X

OCLC:

311056754