Extracellular matrix protocols / edited by Sharona Even-Ram, Vira V. Artym.

Format:

Book

Contributor:

Even-Ram, Sharona.

Artym, Vira V.

Streuli, Charles H.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 522.

Methods in molecular biology, 1064-3745 ; 522

Language:

English

Subjects (All):

Extracellular matrix--Laboratory manuals.

Extracellular matrix.

Extracellular matrix proteins--Laboratory manuals.

Extracellular matrix proteins.

Extracellular Matrix.

Extracellular Matrix Proteins.

Medical Subjects:

Extracellular Matrix.

Extracellular Matrix Proteins.

Genre:

Laboratory manuals.

Physical Description:

xi, 375 pages : illustrations ; 27 cm.

Edition:

Second edition.

Place of Publication:

Totowa, N.J. : Humana ; London : Springer [distributor], [2009]

Summary:

The study of the extracellular matrix has significantly expanded over the past decade. Although thoroughly researched, the complexity and the dynamic nature of the matrix are still under extensive investigation, yielding a growing number of methods of analysis. In Extracellular Matrix Protocols: Second Edition, expert researchers explore this developing field and provide a number of techniques which can be reproduced in most cell biology labs. This edition contains methods that represent cutting edge unique technologies which are well worth exposure to the broad field and may encourage collaboration with expert labs. Chapters include information on molecular biology techniques to study gene expression, biophysical and biochemical methods for the analysis of structure and composition, and cell biology methods for assessment of cell behavior, matrix assembly and tissue engineering applications. Composed in the highly successful Methods in Molecular BiologyTM series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Extracellular Matrix Protocols: Second Edition investigates the diverse roles of the extracellular matrix, providing important tools for all researchers in the ECM community.

Contents:

Part I Molecular Biology

1 Retroviral Delivery of ECM Genes / Vitali Alexeev, Olga Igoucheva 3

2 Tissue-Specific KO of ECM Proteins / Mara Brancaccio, Emila Turco, Emilio Hirsch 15

3 Recombinant Collagen Trimers from Insect Cells and Yeast / Johanna Myllyharju 51

4 Eukaryotic Expression and Purification of Recombinant Extracellular Matrix Proteins Carrying the Strep II Tag / Neil Smyth, Uwe Odenthal, Barbara Merkl, Mats Paulsson 63

5 Preparation of Recombinant Fibronectin Fragments for Functional and Structural Studies / David Staunton, Christopher J. Millard, A. Radu Aricescu, Iain D. Campbell 73

Part II Biochemical and Biophysical Analysis

6 Quantitative Determination of Collagen Cross-links / Nicholas C. Avery, Trevor J. Sims, Allen J. Bailey 103

7 ECM Macromolecules: Height-Mapping and Nano-Mechanics Using Atomic Force Microscopy / Nigel W. Hodson, Cay M. Kielty, Michael J. Sherratt 123

8 Atomic Force Microscopy Measurements of Intermolecular Binding Forces / Gradimir N. Misevic, Yannis Karamanos, Nikola J. Misevic 143

9 Mass-Mapping of ECM Macromolecules by Scanning Transmission Electron Microscopy / Michael J. Sherratt, Helen K. Graham, Cay M. Kielty, David F. Holmes 151

10 Chemical Microscopy of Biological Samples by Dynamic Mode Secondary Ion Mass Spectrometry / Gradimir N. Misevic, Bernard Rasser, Vic Norris, Cédric Dérue, David Gibouin, Fabrice Lefebvre, Marie-Claire Verdus, Anthony Delaune, Guillaume Legent, Camille Ripoll 163

11 ECM Macromolecules: Rotary Shadowing and Transmission Electron Microscopy / Michael J. Sherratt, Roger S. Meadow, Helen K. Graham, Cay M. Kielty, David F. Holmes 175

12 Using Self-Assembled Monolayers to Pattern ECM Proteins and Cells on Substrates / Emanuele Ostuni, George M. Whitesides, Donald E. Ingber, Christopher S. Chen 183

13 Solid Phase Assays for Studying ECM Protein-Protein Interactions / A Paul Mould 195

Part III Cell Biology Assays

14 Cell Adhesion Assays / Martin J. Humphries 203

15 ECM Degradation Assays for Analyzing Local Cell Invasion / Vira V. Artym, Kenneth M. Yamada, Susette C. Mueller 211

16 Fluorescence-Based Assays for In Vitro Analysis of Cell Adhesion and Migration / Paola Spessotto, Katia Lacrima, Pier Andrea Nicolosi, Eliana Pivetta, Martina Scapolan, Roberto Perris 221

17 Fibrin Gel Model for Assessment of Cellular Contractility / Sharona Even-Ram 251

18 Fluorescent Labeling Techniques for Investigation of Fibronectin Fibrillogenesis (Labeling Fibronectin Fibrillogenesis) / Roumen Pankov, Albena Momchilova 261

19 Stromagenesis During Tumorigenesis: Characterization of Tumor-Associated Fibroblasts and Stroma-Derived 3D Matrices / Remedios Castelló-Cros, Edna Cukierman 275

Part IV Organ Models

20 Tissue Recombinants to Study Extracellular Matrix Targeting to Basement Membranes / Patricia Simon-Assmann, Anne-Laure Bolcato-Bellemin, Annick Klein, Michèle Kedinger 309

21 ECM and FGF-Dependent Assay of Embryonic SMG Epithelial Morphogenesis: Investigating Growth Factor/Matrix Regulation of Gene Expression During Submandibular Gland Development / Ivan T. Rebustini, Matthew P. Hoffman 319

22 Analyzing how Cell Adhesion Controls Mammary Gland Function by Transplantation of Embryonic Mammary Tissue from Knockout Mice / Teresa C.M. Klinowska, Charles H. Streuli 331

Part V Tissue Engineering

23 Characterizing ECM Production by Cells Encapsulated in Hydrogels / Iossif A. Strehin, Jennifer H. Elisseeff 349

24 Tissue Engineering and Cell-Populated Collagen Matrices / Paul D. Kemp 363.

Notes:

"Springer protocols"--Cover.

Previous ed.: edited by Charles H. Streuli and Michael E. Grant. Totowa, N.J.: Humana, 2000.

Includes bibliographical references and index.

Related Work:

Streuli, Charles H. Extracellular matrix protocols.

ISBN:

9781588299840

1588299848

OCLC:

262719463