Therapeutic antibodies : methods and protocols / edited by Antony S. Dimitrov.

Format:

Book

Contributor:

Dimitrov, Antony S.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 525.

Methods in molecular biology, 1064-3745 ; 525

Language:

English

Subjects (All):

Immunoglobulins--Therapeutic use.

Immunoglobulins.

Antibodies, Monoclonal--therapeutic use.

Immunotherapy--methods.

Medical Subjects:

Antibodies, Monoclonal--therapeutic use.

Immunotherapy--methods.

Physical Description:

xviii, 585 pages, 8 unnumbered pages of plates : illustrations (some color) ; 27 cm.

Place of Publication:

Totowa, N.J. : Humana ; London : Springer [distributor], 2009.

Contents:

Therapeutic antibodies: current state and future trends - is a paradigm change coming soon? / D.S. Dimitrov and J.D. Marks

Preparation of recombinant viral glycoproteins for novel and therapeutic antibody discovery / Y.P. Chan ... [et al.]

Design of a human synthetic combinatorial library of single-chain antibodies / L. Nahary and I. Benhar

Construction of a human antibody domain (V(H)) library / W. Chen ... [et al.]

Generation and selection of rabbit antibody libraries by phage display / C. Rader

Construction of a large naive human phage-displayed Fab library through one-step cloning / Z. Zhu and D.S. Dimitrov

Identification of target and function specific antibodies for effective drug delivery / Y. Zhou and J.D. Marks

Screening of specific internalization Fab fragment from human naive phage library by combinational bio-panning / X. Wang and B.B. Cao

Competitive antigen panning for selection of HIV-1 neutralizing human monoclonal antibodies specific for gp41 / M.Y. Zhang and D.S. Dimitrov

Selection of non-aggregating V(H) binders from synthetic V(H) phage-display libraries / M. Arbabi-Ghahroudi, R. Mackenzie and J. Tanha

Isolation of full-length IgG antibodies from combinatorial libraries expressed in Escherichia coli / Y. Mazor ... [et al.]

Multiplexed flow cytometry: high-throughput screening of single-chain antibodies / J. Ayriss ... [et al.]

Human antibody repertoires / P.J. Meijer ... [et al.]

Rapid screening platform for stabilization of scFvs in Escherichia coli / B.R. Miller, S.M. Glaser and S.J. Demarest

In vitro antibody affinity maturation targeting germline hotspots / M. Ho and I. Pastan

Affinity maturation by phage display / H. Thie ... [et al.]

Production of chimeric heavy-chain antibodies / J. Zhang, R. Mackenzie and Y. Durocher

Mammalian cell display for antibody engineering / M. Ho and I. Pastan

Improving antibody binding affinity and specificity for therapeutic development / J. Bostrom ... [et al.]

Construction and production of an IgG-like tetravalent bispecific antibody for enhanced therapeutic efficacy / D. Lu and Z. Zhu

Deimmunization of monoclonal antibodies / T.D. Jones ... [et al.]

Anti-CD22 onconase: preparation and characterization / D.L. Newton, L.H. Stockwin and S.M. Rybak

Antibody-cytotoxic agent conjugates: preparation and characterization / R. Singh and H.K. Erickson

High-level production of a humanized immunoRNase fusion protein from stably transfected myeloma cells / J. Krauss ... [et al.]

Antibody fragment expression and purification / D. Dimitrova, V. Choudhry and C.C. Broder

Scaling-up and production of therapeutic antibodies for preclinical studies / Y. Feng and D.S. Dimitrov

Antibody production by the gram-positive bacterium Bacillus megaterium / E. Jordan ... [et al.]

Neutralization of HIV by antibodies / I. Prado, T.R. Fouts and A.S. Dimitrov

Dynamics of antibody domains studied by solution NMR / B.K. Vu ... [et al.]

In vivo method for establishing synergy between antibodies to epidermal growth factor receptor and vascular endothelial growth factor receptor-2 / J.R. Tonra ... [et al.]

Passive immunization with human neutralizing monoclonal antibodies against HIV-1 in macaque models: experimental approaches / R.M. Ruprecht

Organizing, exploring, and analyzing antibody sequence data: the case for relational-database managers / J. Owens.

Notes:

"Springer protocols"--Cover.

Includes bibliographical references and index.

ISBN:

9781934115923

1934115924

OCLC:

262720581