The nuclear receptor superfamily : methods and protocols / edited by Iain J. McEwan.

Format:

Book

Contributor:

McEwan, I. J.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 505.

Methods in molecular biology ; 505

Language:

English

Subjects (All):

Nuclear receptors (Biochemistry).

Receptors, Cytoplasmic and Nuclear--physiology.

Medical Subjects:

Receptors, Cytoplasmic and Nuclear--physiology.

Genre:

Laboratory manuals.

Physical Description:

x, 281 pages, 4 unnumbered pages of plates : illustrations (some color) ; 25 cm.

Place of Publication:

Totowa, N.J. : Humana ; London : Springer [distributor], [2009]

Summary:

Acting principally to control patterns of gene expression, nuclear receptors play vital roles during embryonic development and in the regulation of metabolic and reproductive functions in adult life, which proves this superfamily of ligand-activated transcription factors to be a crucial part of biological life. In The Nuclear Receptor Superfamily: Methods and Protocols, expert researchers describe a range of molecular, structural and cell biological techniques currently used to investigate the structure-function of nuclear receptors, together with experimental approaches that may lead to new therapeutic strategies for treating nuclear receptor-associated diseases. Written in the highly successful Methods in Molecular Biology series format, the chapters in this volume contain brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as notes from the experts to highlight tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and easy to use, The Nuclear Receptor Superfamily: Methods and Protocols provides beneficial and time-saving guidance for all those undertaking research in this evergrowing field of study.

Contents:

Nuclear receptors : one big family / Iain J. McEwan

Methods for measuring ligand dissociation and nuclear receptor turnover in whole cells / Elizabeth M. Wilson

Flow cytometry as a tool for measurement of steroid hormone receptor protein expression in leukocytes / Cherie L. Butts and Esther M. Sternberg

X-ray crystallography of agonist/antagonist-bound receptors / Ashley C.W. Pike

FRAP and FRET methods to study nuclear receptors in living cells / Martin E. van Royen ... [et al.]

Receptor-DNA interactions : EMSA and footprinting / Jason T. Read ... [et al.]

Chromatin immunoprecipitation (ChIP) methodology and readouts / Charles E. Massie and Ian G. Mills

Yeast-based reporter assays for the functional characterization of cochaperone interactions with steroid hormone receptors / Heather A. Balsiger and Marc B. Cox

High throughput analysis of nuclear receptor-cofactor interactions / Michael L. Goodson, Behnom Farboud, and Martin L. Privalsky

Binding affinity and kinetic analysis of nuclear receptor/co-regulator interactions using surface plasmon resonance / Derek N. Lavery

Using RNA interference to study protein function / Carol D. Curtis and Ann M. Nardulli

Using intrinsic fluorescence emission spectroscopy to study steroid receptor and coactivator protein conformation dynamics / Kate Watt and Iain J. McEwan

Development of phosphorylation site-specific antibodies to nuclear receptors / Inés Pineda Torra ... [et al.]

Tissue-selective knockouts of steroid receptors : a novel paradigm in the study of steroid action / Karel De Gendt and Guido Verhoeven

Methods for identifying and studying genetic alterations in hormone-dependent cancers / Outi R. Saramäki, Kati K. Waltering, and Tapio Visakorpi.

Notes:

"Springer protocols"--Cover.

Includes bibliographical references and index.

ISBN:

9781603275743

1603275746

9781603275750

1603275754

OCLC:

244314791