Principles of bioseparations engineering / Raja Ghosh.

Format:

Book

Author/Creator:

Ghosh, Raja.

Contributor:

Class of 1891 Department of Arts Fund.

Language:

English

Subjects (All):

Biomolecules--Separation.

Biomolecules.

Biochemical engineering.

Separation (Technology).

Physical Description:

xiv, 265 pages : illustrations ; 24 cm

Place of Publication:

Singapore ; Hackensack, N.J. : World Scientific, [2006]

Summary:

Bioseparations engineering deals with the scientific and engineering principles involved in large-scale separation and purification of biological products. It is a key component of most chemical engineering/biotechnology/bioprocess engineering programmes. This book discusses the underlying principles of bioseparations engineering written from the perspective of an undergraduate course. It covers membrane based bioseparations in much more detail than some of the other books on bioseparations engineering. Based largely on the lecture notes the author developed to teach the course, this book is especially suitable for use as an undergraduate level textbook, as most other textbooks are targeted at graduate students. Book jacket.

Contents:

1 Overview of bioseparations engineering 1

1.2 What is separated in bioseparation? 2

1.3 Economic importance of bioseparation 3

1.4 Nature of bioseparation 4

1.5 Basis of separation in bioseparation processes 5

1.6 Physical forms separated in bioseparation 6

1.7 Bioseparation techniques 8

1.8 The RIPP scheme 9

1.9 Example of bioseparation 10

1.10 Current trends in the bioseparation 10

2 Properties of biological material 13

2.2 Size 13

2.3 Molecular weight 17

2.4 Diffusivity 19

2.5 Sedimentation coefficient 20

2.6 Osmotic pressure 21

2.7 Electrostatic charge 22

2.8 Solubility 23

2.9 Partition coefficient 24

2.10 Light absorption 24

2.11 Fluorescence 25

3 Mass transfer 29

3.2 Molecular diffusion in liquid medium 30

3.3 Measurement of diffusivity 32

3.4 Estimation of diffusivity 33

3.5 Diffusion of solutes in dense solid 35

3.6 Diffusion of solutes in porous solid 35

3.7 Convective mass transfer 37

3.8 Experimental determination of mass transfer coefficient 40

3.9 Estimation of mass transfer coefficient 41

3.10 Inter-phase mass transfer 42

3.11 Unsteady state mass transfer 42

3.12 Equilibrium and rate processes 45

4 Cell disruption 49

4.2 Cells 49

4.3 Cell disruption using bead mill 53

4.4 Cell disruption using rotor-stator mill 56

4.5 Cell disruption using French press 56

4.6 Cell disruption using ultrasonic vibrations 58

4.7 Cell disruption using detergents 61

4.8 Cell disruption using enzymes 61

4.9 Cell disruption using organic solvents 62

4.10 Cell disruption by osmotic shock 62

5 Precipitation 65

5.2 Factors utilized for precipitation 66

5.3 Precipitation using organic solvents 69

5.4 Precipitation using anti-chaotropic salts 72

5.5 Mechanism of precipitate formation 74

6 Centrifugation 79

6.2 Laboratory centrifuge 80

6.3 Preparative centrifuge 83

6.4 Ultracentrifugation 87

7 Extraction 89

7.2 Solvent systems 89

7.3 Theory of extraction 90

7.4 Aqueous two-phase extraction 93

7.5 Batch extraction 95

7.6 Single-stage continuous extraction using immiscible solvents 95

7.7 Batch extraction using partially miscible solvents 102

7.8 Cross-current continuous extraction using immiscible solvents 103

7.9 Staged counter-current extraction 104

7.10 Differential extraction 109

7.11 Supercritical fluid extraction 113

8 Adsorption 121

8.2 Adsorbents 122

8.3 Separation mechanisms 123

8.4 Adsorption isotherms 132

8.5 Diffusional limitations in adsorption processes 135

8.6 Batch adsorption 136

8.7 Packed bed adsorption 140

8.8 Other types of adsorption devices 147

9 Chromatography 151

9.2 Chromatography system 152

9.3 Theory of chromatography 158

9.4 Shape and yield of a chromatographic peak 167

9.5 Binary chromatography 173

9.6 Hydrodynamic chromatography 176

10 Filtration 181

10.2 Theory of filtration 181

10.3 Filter medium 183

10.4 Driving force 183

10.5 Constant pressure cake filtration 184

10.6 Constant rate cake filtration 190

10.7 Improvement of filtration efficiency 191

10.8 Mode of operation 192

10.9 Filtration equipment 194

11 Membrane based bioseparation 199

11.2 Classification of membrane processes 205

11.3 Membrane equipment 206

11.4 Ultrafiltration 211

11.5 Microfiltration 232

11.6 Dialysis 235

11.7 Liquid membrane processes 240

11.8 Membrane chromatography 242

12 Miscellaneous bioseparation processes 251

12.2 Electrophoresis 251

12.3 Affinity ultrafiltration 255

12.4 Field-flow fractionation 255.

Notes:

Includes bibliographical references and index.

Local Notes:

Acquired for the Penn Libraries with assistance from the Class of 1891 Department of Arts Fund.

ISBN:

9812568921

9789812568922

OCLC:

74874023