DNA and RNA profiling in human blood : methods and protocols / edited by Peter Bugert.

Format:

Book

Contributor:

Bugert, Peter.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 496.

Methods in molecular biology, 1064-3745 ; 496

Language:

English

Subjects (All):

DNA--Analysis.

DNA.

RNA--Analysis.

RNA.

Blood--Analysis.

Blood.

DNA Fingerprinting--methods.

RNA--analysis.

Blood Chemical Analysis--methods.

Medical Subjects:

DNA Fingerprinting--methods.

RNA--analysis.

Blood Chemical Analysis--methods.

Physical Description:

xi, 341 pages, 1 unnumbered leaf of plates : illustrations (some color) ; 27 cm.

Place of Publication:

New York : Humana Press, [2009]

Summary:

Blood samples have consistently proven to be a key source of genetic material for a wide variety of diagnostic or research purposes. In DNA and RNA Profiling in Human Blood: Method and Protocols, leading international experts contribute both established and recently developed protocols for complex and high-throughput DNA and RNA profiling. Divided into two thorough sections, the volume concentrates on DNA profiling for blood cell antigens through methods on high-throughput multiplex approaches and SNP typing, along with RNA profiling in blood cells addressing certain blood cell types such as platelets, reticulocytes, and megakaryocytes. Written in the highly successful Methods in Molecular Biology series format, all of the chapters include brief introductions on the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as the Notes section which highlights tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, DNA and RNA Profiling in Human Blood: Methods and Protocols is an ideal guide to the molecular profiling approaches that have opened up this broad field of research and have shown great promise in the further identifying of disease markers in blood.

Contents:

Part I DNA Profiling for Blood Cell Antigens

1 PCR-ELISA for High-Throughput Blood Group Genotyping / Maryse St-Louis 3

2 Single Base Extension in Multiplex Blood Group Genotyping / Gregory A. Denomme 15

3 Real-Time PCR Assays for High-Throughput Blood Group Genotyping / Fernando Araujo 25

4 Real-Time PCR Assays for High-Throughput Human Platelet Antigen Typing / Simon E. McBride 39

5 Multiplex ABO Genotyping by Minisequencing / Gianmarco Ferri, Susi Pelotti 51

6 Multiplex Genotyping for Thrombophilia-Associated SNPs by Universal Bead Arrays / Susan Bortolin 59

7 Pyrosequencing of Toll-Like Receptor Polymorphisms of Functional Relevance / Parviz Ahmad-Nejad 73

8 Multiplex HLA-Typing by Pyrosequencing / Ying Lu, Julian Boehm, Lynn Nichol, Massimo Trucco, Steven Ringquist 89

9 High-Throughput Multiplex HLA-Typing by Ligase Detection Reaction (LDR) and Universal Array (UA) Approach / Clarissa Consolandi 115

10 Medium- to High-Throughput SNP Genotyping Using VeraCode Microbeads / Charles H. Lin, Joanne M. Yeakley, Timothy K. McDaniel, Richard Shen 129

11 The Use of Maternal Plasma for Prenatal RhD Blood Group Genotyping / Kirstin Finning, Pete Martin, Geoff Daniels 143

Part II RNA Profiling in Bloods Cells

12 Nanoliter High-Throughput PCR for DNA and RNA Profiling / Colin J. H. Brenan, Douglas Roberts, James Hurley 161

13 RNA Stabilization of Peripheral Blood and Profiling by Bead Chip Analysis / Svenja Debey-Pascher, Daniela Eggle, Joachim L. Schultze 175

14 RNA Profiling in Peripheral Blood Cells by Fluorescent Differential Display PCR / Martin Steinau, Mangalathu S. Rajeevan 211

15 cDNA Amplification by SMART-PCR and Suppression Subtractive Hybridization (SSH)-PCR / Andrew Hillmann, Eimear Dunne, Dermot Kenny 223

16 Transcript Profiling of Human Platelets Using Microarray and Serial Analysis of Gene Expression (SAGE) / Dmitri V. Gnatenko, John J. Dunn, John Schwedes, Wadie F. Bahou 245

17 Genome-Wide Platelet RNA Profiling in Clinical Samples / Angelika Schedel, Nina Rolf 273

18 PCR-Based Amplification of Platelet mRNA Sequences Obtained From Small-Scale Platelet Samples / Jutta M. Rox, Jens Muller, Bernd Potzsch 285

19 MicroRNA Profiling of Megakaryocytes / Ramiro Garzon 293

20 Serial Analysis of Gene Expression Adapted for Downsized Extracts (SAGE/SADE) Analysis in Reticulocytes / Beatrice Bonafoux, Therese Commes 299

21 Real-Time PCR Analysis for Blood Cell Lineage Specific Markers / Louise Edvardsson, Tor Olofsson 313

22 Monitoring the Immune Response Using Real-Time PCR / Patrick Stordeur 323.

Notes:

"Springer protocols"--Cover.

Includes bibliographical references and index.

ISBN:

9781934115930

1934115932

OCLC:

233934524