Macromolecular drug delivery : methods and protocols / edited by Mattias Belting.

Format:

Book

Contributor:

Belting, Mattias.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 480.

Methods in molecular biology, 1064-3745 ; 480

Language:

English

Subjects (All):

Drug delivery systems.

Drug Delivery Systems.

Macromolecular Substances.

Medical Subjects:

Drug Delivery Systems.

Macromolecular Substances.

Genre:

Laboratory manuals.

Physical Description:

xiv, 202 pages : illustrations (some color) ; 25 cm.

Place of Publication:

New York, NY : Humana Press, [2009]

Summary:

Macromolecular drugs hold the promise of becoming new therapeutics for several major disorders, including cancer and cardiovascular disease. This incredible potential is explored in Macromolecular Drug Delivery, a volume which gives a wide-ranging overview of contemporary methods used in the field, and which addresses the limitations presented by a lack of safe and efficient drug delivery strategies. Chapters offer information on both in vitro and in vivo methods of macromolecular delivery, thus appealing to a broad scientific audience. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Macromolecular Drug Delivery offers a platform for interdisciplinary collaboration, which should provide opportunities for new discoveries at the interface between disciplines. Ultimately, this cooperation will lead to the use of macromolecules as novel diagnostic tools and, even more importantly, as a means to revolutionize the way we view and treat diseases.

Contents:

Developments in macromolecular drug delivery / Mattias Belting and Anders Wittrup

Synthetic vs. natural/biodegradable polymers for delivery of shRNA-based cancer therapies / John S. Vorhies and John J. Nemunaitis

siRNA and DNA transfer to cultured cells / Bagavathi Gopalakrishnan and Jon Wolff

Poly ([beta]-amino esters) : procedures for synthesis and gene delivery / Jordan J. Green ... [et al.]

Systemic delivery and pre-clinical evaluation of nanoparticles containing antisense oligonucleotides and siRNAs / Chuanbo Zhang ... [et al.]

Peptide-based delivery of steric-block PNA oligonucleotides / Saïd Abes ... [et al.]

Characterizing peptide-mediated DNA internalization in human cancer cells / Anders Wittrup and Mattias Belting

Selection and characterization of antibodies from phage display libraries against internalizing membrane antigens / Johan Fransson and Carl A.K. Borrebaeck

Artificial membrane models for the study of macromolecular delivery / Lena Mäler and Astrid Gräslund

Enhanced delivery of macromolecules into cells by electroendocytosis / Alexander Barbul ... [et al.]

In vitro systems for studying epithelial transport of macromolecules / Nicole Daum ... [et al.]

Preparation of macromolecule-containing dry powders for pulmonary delivery / Kelly S. Kraft and Marshall Grant

Macromolecular delivery across the blood-brain barrier / Kullervo Hynynen

Positron emission tomography (PET) and macromolecular delivery in vivo / Ludwig G. Strauss and Antonia Dimitrakopoulou-Strauss.

Notes:

"Springer protocols"--Cover.

Includes bibliographical references and index.

ISBN:

9781588299994

1588299996

OCLC:

227033891