Membrane trafficking / edited by Ales Vancura.

Format:

Book

Contributor:

Vancura, Ales.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 457.

Methods in molecular biology ; 457

Language:

English

Subjects (All):

Biological transport.

Biological Transport--genetics.

Biological Transport--physiology.

Cell Membrane--genetics.

Cell Membrane--physiology.

Protein Transport--genetics.

Protein Transport--physiology.

Membrane Transport Proteins--analysis.

Cytological Techniques.

Medical Subjects:

Biological Transport--genetics.

Biological Transport--physiology.

Cell Membrane--genetics.

Cell Membrane--physiology.

Protein Transport--genetics.

Protein Transport--physiology.

Membrane Transport Proteins--analysis.

Cytological Techniques.

Genre:

Laboratory manuals.

Physical Description:

xv, 405 pages : illustrations ; 25 cm.

Place of Publication:

Totowa, N.J. : Humana ; London : Springer [distributor], [2008]

Summary:

As membrane trafficking research has expanded over the past thirty years, a remarkable convergence of information has been gained by using genetic approaches in yeast cells with biochemical approaches in mammalian cells. Membrane Trafficking reflects these advances by devoting one section of the book to yeast cells and the other to mammalian cells, with each section providing both classic and cutting-edge techniques to study macromolecular transport across the membranes. These protocols, contributed by top experts in the field, have been tested in laboratories all around world. As a volume of the highly successful Methods in Molecular Biology series, the chapters include step-by-step laboratory protocols, a list of necessary materials and reagents, and the greatly beneficial Notes section, which aids in troubleshooting and avoiding known pitfalls.

Comprehensive and state-of-the-art, Membrane Trafficking is the ideal reference for both senior researchers experienced in the field and graduate students studying membrane trafficking for the first time.

Contents:

Part I Yeast Cells

1 Freight Management in the Cell: Current Aspects of Intracellular Membrane Trafficking / Johannes M. Herrmann, Anne Spang 3

2 High-Throughput Protein Extraction and Immunoblotting Analysis in Saccharomyces cerevisiae / Todd C. Lorenz, Vikram C. Anand, Gregory S. Payne 13

3 Genome-Wide Analysis of Membrane Transport Using Yeast Knockout Arrays / Helen E. Burston, Michael Davey, Elizabeth Conibear 29

4 A Cell-Free System for Reconstitution of Transport Between Prevacuolar Compartments and Vacuoles in Saccharomyces cerevisiae / Thomas A. Vida 41

5 In vitro Analysis of the Mitochondrial Preprotein Import Machinery Using Recombinant Precursor Polypeptides / Dorothea Becker, Martin Krayl, Wolfgang Voos 59

6 In Vitro Import of Proteins Into Isolated Mitochondria / Karl Bihlmaier, Melanie Bien, Johannes M. Herrmann 85

7 Synthesis and Sorting of Mitochondrial Translation Products / Heike Bauerschmit, Soledad Funes, Johannes M. Herrmann 95

8 In Vivo Labeling and Analysis of Mitochondrial Translation Products in Budding and in Fission Yeasts / Karine Gouget, Fulvia Verde, Antoni Barrientos 113

9 Exploring Protein-Protein Interactions Involving Newly Synthesized Mitochondrial DNA-Encoded Proteins / Darryl Horn, Flavia Fontanesi, Antoni Barrientos 125

10 Purification of Yeast Membranes and Organelles by Sucrose Density Gradient Centrifugation / Jennifer Chang, Victoria Ruiz, Ales Vancura 141

11 Microscopic Analysis of Lipid Droplet Metabolism and Dynamics in Yeast / Heimo Wolinski, Sepp D. Kohlwein 151

12 Use of Bimolecular Fluorescence Complementation in Yeast Saccharomyces cerevisiae / Kari-Pekka Skarp, Xueqiang Zhao, Marion Weber, Jussi Jantti 165

Part II Mammalian Cells

13 Using Quantitative Fluorescence Microscopy to Probe Organelle Assembly and Membrane Trafficking / Brian Storrie, Tregei Starr, Kimberly Forsten-Williams 179

14 Measuring Secretory Membrane Traffic / Vytaute Starkuviene, Arne Seitz, Holger Erfle, Rainer Pepperkok 193

15 A Correlative Light and Electron Microscopy Method Based on Laser Micropatterning and Etching / Julien Colombelli, Carolina Tangemo, Uta Haselman, Claude Antony, Ernst H.K. Stelzer, Rainer Pepperkok, Emmanuel G. Reynaud 203

16 Approaches to Investigate the Role of Signaling in ER-to-Golgi Transport / Laura J. Sharpe, Ximing Du, Andrew J. Brown 215

17 Recruitment of Coat Proteins to Peptidoliposomes / Gregor Suri, Martin Spiess, Pascal Crottet 227

18 SNARE-Mediated Fusion of Liposomes / Jerome Vicogne, Jeffrey E. Pessin 241

19 Use of Polarized PC12 Cells to Monitor Protein Localization in the Early Biosynthetic Pathway / Ragna Sannerud, Michael Marie, Bodil Berger Hansen, Jaakko Saraste 253

20 Tracking the Transport of E-Cadherin to and From the Plasma Membrane / Matthew P. Wagoner, Kun Ling, Richard A. Anderson 267

21 Analysis of Nucleocytoplasmic Shuttling of NF[kappa]B Proteins in Human Leukocytes / Chandra C. Ghosh, Hai-Yen Vu, Tomas Mujo, Ivana Vancurova 279

22 Imaging pHluorin-Based Probes at Hippocampal Synapses / Stephen J. Royle, Bjorn Granseth, Benjamin Odermatt, Aude Derevier, Leon Lagnado 293

23 Analysis of Receptor Tyrosine Kinase Internalization Using Flow Cytometry / Ning Li, Kristen S. Hill, Lisa A. Elferink 305

24 Measurement of Receptor Endocytosis and Recycling / Jane M. Knisely, Jiyeon Lee, Guojun Bu 319

25 Styryl Dye-Based Synaptic Vesicle Recycling Assay in Cultured Cerebellar Granule Neurons / Victor Anggono, Michael A. Cousin, Phillip J. Robinson 333

26 Use of Quantitative Immunofluorescence Microscopy to Study Intracellular Trafficking: Studies of the GLUT4 Glucose Transporter / Vincent Blot, Timothy E. McGraw 347

27 Dissecting GLUT4 Traffic Components in L6 Myocytes by Fluorescence-Based, Single-Cell Assays / Costin N. Antonescu, Varinder K. Randhawa, Amira Klip 367

28 Functional Genetic Analysis of the Mammalian Mitochondrial DNA Encoded Peptides / Maria Pilar Bayona-Bafaluy, Nieves Movilla, Acisclo Perez-Martos, Patricio Fernandez-Silva, Jose Antonio Enriquez 379.

Notes:

Includes bibliographical references and index.

ISBN:

9781588299253

1588299252

OCLC:

182528165