SARS and other coronaviruses : laboratory protocols / edited by Dave Cavanagh.

Format:

Book

Contributor:

Cavanagh, David, Ph. D.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 454.

Methods in molecular biology ; 454

Language:

English

Subjects (All):

Coronavirus infections--Laboratory manuals.

Coronavirus infections.

Coronavirus infections--Pathogenesis--Laboratory manuals.

Severe acute respiratory syndrome-related coronavirus--isolation & purification.

Coronavirus infections--Pathogenesis.

Severe acute respiratory syndrome-related coronavirus--pathogenicity.

Coronavirus--isolation & purification.

Coronavirus--pathogenicity.

Medical Subjects:

Severe acute respiratory syndrome-related coronavirus--isolation & purification.

Severe acute respiratory syndrome-related coronavirus--pathogenicity.

Coronavirus--isolation & purification.

Coronavirus--pathogenicity.

Genre:

Laboratory manuals.

Physical Description:

xv, 344 pages : illustrations ; 25 cm.

Place of Publication:

Totowa, N.J. : Humana ; London : Springer [distributor], [2008]

Summary:

In 2003, the word "coronavirus" spread across the globe, somewhat further than the virus that sparked the panic. In SARS-and Other Coronaviruses: Laboratory Protocols, expert researchers examine these devastating viruses through detailed laboratory protocols. Chapters deal with such subjects as detection and discovery of coronaviruses by nucleic acid and antibody-based approaches, virus isolation, propagation and titration, virus purification, structure analysis by electron cryomicroscopy, expression and crystallization of viral proteins, raising antibodies against viral proteins, manipulation of the coronavirus genome, and descriptions of how to investigate aspects of the cell surface for coronavirus receptors. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, SARS- and Other Coronaviruses: Laboratory Protocols serves as an ideal guide for all virologists and especially for those working with coronaviruses.

Contents:

Part I Detection and Discovery of Coronaviruses

1 A Pancoronavirus RT-PCR Assay for Detection of All Known Coronaviruses / Leen Vijgen, Elien Moes, Els Keyaerts, Sandra Li, Marc Van Ranst 3

2 Detection of Group 1 Coronaviruses in Bats Using Universal Coronavirus Reverse Transcription Polymerase Chain Reactions / Leo L. M. Poon, J. S. Malik Peiris 13

3 Detection and Sequence Characterization of the 3'-End of Coronavirus Genomes Harboring the Highly Conserved RNA Motif s2m / Christine Monceyron Jonassen 27

4 RT-PCR Detection of Avian Coronaviruses of Galliform Birds (Chicken, Turkey, Pheasant) and in a Parrot / Francesca Anne Culver, Paul Britton, Dave Cavanagh 35

5 Detection of Group 2a Coronaviruses with Emphasis on Bovine and Wild Ruminant Strains / Mustafa Hasoksuz, Anastasia Vlasova, Linda J. Saif 43

6 Detection of SARS Coronavirus in Humans and Animals by Conventional and Quantitative (Real Time) Reverse Transcription Polymerase Chain Reactions / J. S. Malik Peiris, Leo L. M. Poon 61

7 Detection of New Viruses by VIDISCA / Krzysztof Pyrc, Maarten F. Jebbink, Ben Berkhout, Lia van der Hoek 73

Part II Isolation, Growth, Titration, and Purification of Coronaviruses

8 Titration of Human Coronaviruses, HCoV-229E and HCoV-OC43, by an Indirect Immunoperoxidase Assay / Francine Lambert, Helene Jacomy, Gabriel Marceau, Pierre J. Talbot 93

9 The Preparation of Chicken Tracheal Organ Cultures for Virus Isolation, Propagation, and Titration / Brenda V. Jones, Ruth M. Hennion 103

10 Isolation and Propagation of Coronaviruses in Embryonated Eggs / James S. Guy 109

11 Large-Scale Preparation of UV-Inactivated SARS Coronavirus Virions for Vaccine Antigen / Yasuko Tsunetsugu-Yokota 119

Part III Structure of Coronaviruses Analyzed by Electron Microscopy

12 Purification and Electron Cryomicroscopy of Coronavirus Particles / Benjamin W. Neuman, Brian D. Adair, Mark Yeager, Michael J. Buchmeier 129

Part IV Expression of Coronavirus Proteins and Crystallization

13 Production of Coronavirus Nonstructural Proteins in Soluble Form for Crystallization / Yvonne Piotrowski, Rajesh Ponnusamy, Stephanie Glaser, Anniken Daabach, Ralf Moll, Rolf Hilgenfeld 139

Part V Raising Antibodies to Coronavirus Proteins

14 Generating Antibodies to the Gene 3 Proteins of Infectious Bronchitis Virus / Amanda R. Pendleton, Carolyn E. Machamer 163

15 Establishment and Characterization of Monoclonal Antibodies Against SARS Coronavirus / Kazuo Ohnishi 191

16 Production of Monospecific Rabbit Antisera Recognizing Nidovirus Proteins / Jessika C. Zevenhoven-Dobbe, Alfred L. M. Wassenaar, Yvonne van der Meer, Eric J. Snijder 205

Part VI Manipulating the Genomes of Coronaviruses

17 Manipulation of the Coronavirus Genome Using Targeted RNA Recombination with Interspecies Chimeric Coronaviruses / Cornelis A.M. de Haan, Bert Jan Haijema, Paul S. Masters, Peter J.M. Rottier 229

18 Generation of Recombinant Coronaviruses Using Vaccinia Virus as the Cloning Vector and Stable Cell Lines Containing Coronaviral Replicon RNAs / Klara Kristin Eriksson, Divine Makia, Volker Thiel 237

19 Transient Dominant Selection for the Modification and Generation of Recombinant Infectious Bronchitis Coronaviruses / Maria Armesto, Rosa Casais, Dave Cavanagh, Paul Britton 255

20 Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomes / Fernando Almazan, Carmen Galan, Luis Enjuanes 275

21 Systematic Assembly and Genetic Manipulation of the Mouse Hepatitis Virus A59 Genome / Eric F. Donaldson, Amy C. Sims, Ralph S. Baric 293

Part VII Identifying Coronavirus Receptors

22 Identification of Sugar Residues Involved in the Binding of TGEV to Porcine Brush Border Membranes / Christel Schwegmann-Wessels, Georg Herrler 319

23 Pseudotyped Vesicular Stomatitis Virus for Analysis of Virus Entry Mediated by SARS Coronavirus Spike Proteins / Shuetsu Fukushi, Rie Watanabe, Fumihiro Taguchi 331.

Notes:

Includes bibliographical references and index.

ISBN:

9781588298676

1588298671

OCLC:

192027781