Cell fusion : overviews and methods / edited by Elizabeth H. Chen.

Format:

Book

Contributor:

Chen, Elizabeth H.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 475.

Methods in molecular biology ; 475

Language:

English

Subjects (All):

Cell hybridization.

Molecular biology.

Cell Fusion.

Cell Fusion--methods.

Medical Subjects:

Cell Fusion.

Cell Fusion--methods.

Physical Description:

xi, 421 pages, 4 unnumbered pages of plates : illustrations (some color) ; 25 cm.

Place of Publication:

Totowa, N.J. : Humana ; London : Springer [distributor], [2008]

Summary:

Exciting work in the past decade has revealed commonalities and differences among individual cell fusion events. In Cell Fusion: Overviews and Methods, a team of leading experts provide a collection of overviews that outline our current understanding of cell fusion and methods that present classic and state-of-the-art experimental approaches in a variety of systems. Divided into two convenient parts, the volume begins with nine overviews that describe different cell fusion events in models from yeast to mammals, and it continues with thirteen chapters illustrating commonly used methods to assay cell fusion in particular systems. As a part of the highly successful Methods in Molecular Biology series, these methods chapters compile step-by-step, readily reproducible protocols with lists of the necessary materials and reagents, along with tips on troubleshooting and avoiding known pitfalls. Cutting-edge and user-friendly, Cell Fusion: Overviews and Methods serves as a comprehensive resource for anyone, expert or novice, interested in the fascinating biological process of cell fusion.

Contents:

Part I Cell Fusion

1 Yeast Mating: A Model System for Studying Cell and Nuclear Fusion / Casey A. Ydenberg, Mark D. Rose 3

2 Cell Fusion in the Filamentous Fungus, Neurospora crassa / Andre Fleissner, Anna R. Simonin, N. Louise Glass 21

3 Gametic Cell Adhesion and Fusion in the Unicellular Alga Chlamydomonas / Nedra F. Wilson 39

4 Cell Fusion in Caenorhabditis elegans / Scott Alper, Benjamin Podbilewicz 53

5 Myoblast Fusion in Drosophila / Susan M. Abmayr, Shufei Zhuang, Erika R. Geisbrecht 75

6 Mammalian Fertilization Is Dependent on Multiple Membrane Fusion Events / Paul M. Wassarman, Eveline S. Litscher 99

7 Molecular Control of Mammalian Myoblast Fusion / Katie M. Jansen, Grace K. Pavlath 115

8 Placenta Trophoblast Fusion / Berthold Huppertz, Marcus Borges 135

9 Macrophage Fusion: Molecular Mechanisms / Agnes Vignery 149

Part II Cell Fusion Assays

10 Cell Fusion Assays for Yeast Mating Pairs / Eric Grote 165

11 Ultrastructural Analysis of Cell Fusion in Yeast / Alison E. Gammie 197

12 Isolation and In Vitro Binding of Mating Type Plus Fertilization Tubules From Chlamydomonas / Nedra F. Wilson 213

13 Optical Imaging of Cell Fusion and Fusion Proteins in Caenorhabditis elegans / Star Ems, William A. Mohler 223

14 Ultrastructural Imaging of Cell Fusion in Caenorhabditis elegans / Star Ems, William A. Mohler 245

15 Live Imaging of Drosophila Myoblast Fusion / Brian E. Richardson, Karen Beckett, Mary K. Baylies 263

16 Ultrastructural Analysis of Myoblast Fusion in Drosophila / Shiliang Zhang, Elizabeth H. Chen 275

17 A Genomic Approach to Myoblast Fusion in Drosophila / Beatriz Estrada, Alan M. Michelson 299

18 Mesenchymal Cell Fusion in the Sea Urchin Embryo / Paul G. Hodor, Charles A. Ettensohn 315

19 Sperm-Egg Fusion Assay in Mammals / Naokazu Inoue, Masaru Okabe 335

20 Quantitative Assays for Cell Fusion / Jessica H. Shinn-Thomas, Victoria L. Scranton, William A. Mohler 347

21 Fusion Assays and Models for the Trophoblast / Sascha Drewlo, Dora Baczyk, Caroline Dunk, John Kingdom 363

22 Methods to Fuse Macrophages In Vitro / Agnes Vignery 383.

Notes:

Includes bibliographical references and index.

ISBN:

9781588299116

1588299112

OCLC:

222160298