Programmed cell death. Part B, The biology and therapeutic implications of cell death / edited by Roya Khosravi-Far ... [and others].

Format:

Book

Contributor:

Khosravi-Far, Roya.

Series:

Methods in enzymology 0076-6879 ; v. 446.

Methods in enzymology, 0076-6879 ; v. 446

Language:

English

Subjects (All):

Apoptosis.

Cancer cells--Variation.

Cancer cells.

Medical Subjects:

Apoptosis.

Physical Description:

xlviii, 450 pages, 6 unnumbered pages of plates : illustrations (some color) ; 24 cm.

Place of Publication:

San Diego, Calif : Academic Press/Elsevier, [2008]

Contents:

1 Analysis of Autophagic and Necrotic Cell Death in Dictyostelium / Corinne Giusti, Artemis Kosta, David Lam, Emillie Tresse, Marie-Francoise Luciani, Pierre Golstein 1

2 Growing Dictyostelium Cells in Monolayer Cultures 3

3 Inducing Autophagic Vacuolar Cell Death 4

4 Further Exploration of Autophagic Cell Death 6

5 Inducing Necrotic Cell Death 10

6 Further Exploration of Necrotic Cell Death 10

2 Methods and Protocols for Studying Cell Death in Drosophila / Donna Denton, Kathryn Mills, Sharad Kumar 17

2 Cell Death Analysis During Development 22

3 Biochemical Analysis 27

4 Genetic Analysis 31

3 In Vivo and In Vitro Methods for Studying Apoptotic Cell Engulfment in Drosophila / Elizabeth A. Silva, Jemima Burden, Nathalie C. Franc 39

2 Culture Conditions 41

3 Labeling of Apoptotic Cells 43

4 Visualizing the Macrophages 46

5 Quantification and Statistics 50

6 Engulfment in Cell Culture 52

4 A Mouse Mammary Epithelial Cell Model to Identify Molecular Mechanisms Regulating Breast Cancer Progression / Vassiliki Karantza-Wadsworth, Eileen White 61

2 A Novel Mouse Mammary Epithelial Cell Model 63

3 Protocols 65

5 Immortalized Mouse Epithelial Cell Models to Study the Role of Apoptosis in Cancer / Robin Mathew, Kurt Degenhardt, Liti Haramaty, Cristina M. Karp, Eileen White 77

2 Immortalization of Baby Mouse Kidney, Mammary, Prostate, and Ovarian Surface Epithelial Cells 81

3 Development of iBMK Cell Lines for Identifying the Role of Apoptosis in Cancer 94

4 Aposcreen: An iBMK Cell-Based Screen for the Identification of Apoptosis-Inducing Compounds as Potential Anti-Cancer Agents 95

5 Adaptation of the iBMK Cell Model System for the Evaluation of Tissue-Specific Tumor-Promoting Functions 97

6 Tumorigenicity Assay 100

7 Noninvasive In Vivo Monitoring of Tumor Growth by Expression of Fluorescence and Chemiluminescence Markers 102

8 Tumor-Derived Cell Lines (TDCLs) as a Model System for Epithelial Tumor-Promoting Functions 103

9 Concluding Remarks and Future Perspectives 104

6 DNA Damage Response and Apoptosis / Dragos Plesca, Suparna Mazumder, Alexandru Almasan 107

2 Methods 110

7 Phorbol Ester-Induced Apoptosis and Senescence in Cancer Cell Models / Liqing Xiao, M. Cecilia Caino, Vivian A. von Burstin, Jose L. Oliva, Marcelo G. Kazanietz 123

2 PKC[delta] as a Pro-Apoptotic Kinase 124

3 PKC[delta] as a Mediator of Apoptotic Responses in Prostate Cancer Cells: The Emergence of an Autocrine Paradigm 125

4 Roles of PKC[alpha] and PKC[epsilon] in LNCaP Cell Death and Survival 127

5 Regulation of Cell Cycle and Senescence by PKC 128

6 Determination of Phorbol Ester-Induced Apoptosis in Prostate Cancer Cells 129

7 Adenoviral Expression of PKC Isozymes 131

8 RNA Interference of PKC Isozymes 132

9 Collection and Storage of CM from LNCaP Cells 133

10 Determination of Senescence in Respose to Phorbol Esters 134

8 Manipulation of PKC Isozymes by RNA Interference and Inducible Expression of PKC Constructs / Alakananda Basu, Shalini D. Persaud, Usha Sivaprasad 141

2 Methods 143

9 Studying p53-Dependent Cell Death In Vitro and In Vivo / Kageaki Kuribayashi, Niklas Finnberg, Wafik S. El-Deiry 159

2 Studying p53-Dependent Cell Death In Vitro 160

3 Overexpression of p53 by Adenovirus (Ad) 160

4 Silencing the p53 Gene by Retroviral Vector 164

5 Studying the Apoptotic Response 165

6 Studying p53-Dependent Apoptosis In Vivo 165

10 NF-[kappa]B as a Determinant of Distinct Cell Death Pathways / Irene L. Ch'en, Stephen M. Hedrick, Alexander Hoffmann 175

2 Methods 178

3 DNA 180

4 Proteins 183

5 Metabolic Health 184

6 Senescence Detection 185

11 Purification and Bioassay of Hedgehog Ligands for the Study of Cell Death and Survival / Pilar Martinez-Chinchilla, Natalia A. Riobo 189

2 Hedgehog and the Regulation of Cell Death 193

3 Generation of Recombinant Hedgehog Ligands 195

12 Experimental Approaches to Investigate the Proteasomal Degradation Pathways Involved in Regulation of Apoptosis / Alan Tseng, Hiroyuki Inuzuka, Daming Gao, Amrik Singh, Wenyi Wei 205

2 General Instructions 207

3 Protocols 207

13 Ubiquitination Mediated by Inhibitor of Apoptosis Proteins / Sun-Mi Park, Shimin Hu, Tae H. Lee, Xiaolu Yang 225

2 In Vitro Ubiquitination Assay 226

3 In Vivo Ubiquitination Assay 232

14 Phosphatases and Regulation of Cell Death / Chi-Wu Chiang, Ling Yan, Elizabeth Yang 237

2 Identification of the Putative Phosphatase in Cells 240

3 In Vitro Phosphatase Assays 248

4 Interaction of the Phosphatase and Apoptotic Protein 249

5 Linking Phosphatase Activity to Cell Death Protein Function 253

15 Analysis of Neuronal Cell Death in Mammals / Marcello D'Amelio, Virve Cavallucci, Adamo Diamantini, Francesco Cecconi 259

2 Apoptosis Detection In Vitro 260

3 Assessment of Cell Death 262

4 Apoptosis Detection in Brain Development 269

5 Detection of Synaptic Apoptosis (Synaptosis) 272

16 Dissecting Apoptosis and Intrinsic Death Pathways in the Heart / Danielle Weidman, James Shaw, Joseph Bednarczyk, Kelly M. Regula, Natalia Yurkova, Tong Zhang, Floribeth Aguilar, Lorrie A. Kirshenbaum 277

2 Protocols 279

17 Quantification of Vascular Endothelial Cell Apoptosis In Vivo / Xuefeng Zhang, Sareh Parangi 287

2 Protocols 288

18 Recombinant TRAIL and TRAIL Receptor Analysis / Nicholas Harper, Marion MacFarlane 293

2 Generation of Recombinant TRAIL 295

3 Analysis of TRAIL Receptors 302

19 Analysis of Tnf-Related Apoptosis-Inducing Ligand In Vivo Through Bone Marrow Transduction and Transplantation / Keli Song, Raj Mariappan, Roya Khosravi-Far 315

2 Generation of Retroviral Infection Medium 317

3 Bone Marrow Cell Preparation 318

4 Retroviral Transduction of Bone Marrow Cells 319

5 Bone Marrow Transplantation 321

6 Assessment of Bone Marrow Transplantation 322

7 Pathologic Evaluation of TRAIL-Transduced Mice 323

8 Methods for Immunohistochemical Staining for TRAIL Expression 326

9 Applications that Use the Bone Marrow Transplantation Approach 328

20 Overcoming Resistance to TRAIL-Induced Apoptosis in Prostate Cancer by Regulation of c-FLIP / Xiaoping Zhang, Wenhua Li, Aria F. Olumi 333

2 Structure and Molecular Mechanisms of c-FLIP in Apoptosis 334

3 Expression of c-FLIP 335

4 Regulation of c-FLIP Expression 337

5 Targeting c-FLIP to Enhance Apoptosis in Cancer Cells 341

6 Methods and Materials 342

21 Caspase Assays: Identifying Caspase Activity and Substrates In Vitro and In Vivo / Cristina Pop, Guy S. Salvesen, Fiona L. Scott 351

2 Caspase Methods 353

22 Synthesis and Biophysical Characterization of Stabilized [alpha]-Helices of BCL-2 Domains / Gregory H. Bird, Federico Bernal, Kenneth Pitter, Loren D. Walensky 369

2 Synthesis of Non-Natural Amino Acids for Peptide Stapling 372

3 Design and Synthesis of Stabilized [alpha]-Helices of BCL-2 Domains (SAHBs) 373

4 Structural Assessment of SAHBs by Circular Dichroism (CD) 376

5 Protease Resistance Testing of SAHBs 378

6 Cell Permeability Screening of SAHBs 381

23 Dissection of the BCL-2 Family Signaling Network with Stabilized [alpha]-Helices of BCL-2 Domains / Kenneth Pitter, Federico Bernal, James LaBelle, Loren D. Walensky 387

2 BCL-2 Family Binding Measurements by Fluorescence Polarization Assay 388

3 In Vitro Release Assays as a Measure of Pro-Apoptotic Activity 392

4 Measurement of Cellular Apoptosis Induction 400

5 Identification of In Situ Mechanistic Targets of SAHBs 403

24 ERM-Mediated Genetic Screens in Mammalian Cells / Dan Liu, Zhou Songyang 409

2 Methods and Design 411

3 ERM-Mediated Genetic Screens and Re-PCA 415.

Notes:

Includes bibliographical references and index.

ISBN:

9780123744647

0123744644

OCLC:

244287019